乙酰水杨酸联合双氯芬酸治疗骨关节炎的机制

doi:10.3969/j.issn.2097-0005.2021.06.002

betway必威登陆网址（betway.com ）学报 ›› 2021, Vol. 42 ›› Issue (6): 406-410.DOI: 10.3969/j.issn.2097-0005.2021.06.002

乙酰水杨酸联合双氯芬酸治疗骨关节炎的机制

侯圣贵, 刘建强

济南市第四人民医院,山东济南 250033

收稿日期:2020-12-13 出版日期:2021-06-25 发布日期:2021-08-25
通讯作者: 刘建强(1975—),男,副主任医师,博士,研究方向:关节外科,E-mail:jqliu0110@163.com。
作者简介:候圣贵(1966—),副主任药师,本科,主要从事临床药学工作。E-mail:hsgjinan@163.com。
基金资助:
济南市卫生计生委科技立项课题(201601036)。

Mechanism of acetylsalicylic acid combined with diclofenac in the treatment of osteoarthritis

Hou Shenggui, Liu Jianqiang

The Fourth People's Hospital of Jinan City,Jinan 250033,China

Received:2020-12-13 Online:2021-06-25 Published:2021-08-25

摘要/Abstract

摘要： 目的探讨兔骨关节炎模型应用双氯芬酸联合不同浓度乙酰水杨酸对关节软骨病变的治疗机制。方法乙酰水杨酸分为三个浓度组(5 mg/kg、10 mg/kg、20 mg/kg)联合双氯芬酸,在兔的骨关节炎模型中验证治疗作用。对各组兔骨关节炎模型的关节病理进行评分,对比各组骨关节炎兔模型的病变关节液中NO、IL-1β含量,分别检测MMP-3,MMP-13蛋白水平和TIMP-1的mRNA水平在各组之间的差异表达。结果三个浓度组的联合用药均对兔骨关节炎有很好的治疗作用:随着药物浓度的增加,评分分数降低;NO、IL-1β的含量降低;MMP-3和MMP-13的蛋白表达水平减少;TIMP-1的mRNA表达水平上调。结论成功建立兔骨关节炎模型,验证了双氯芬酸联合不同浓度的乙酰水杨酸能够治疗骨关节炎。本研究证实联合药物通过抑制NO、IL-1β、MMP-3、MMP-13的表达,上调TIMP-1,治疗骨关节炎。

关键词: 乙酰水杨酸联合双氯芬酸, 骨关节炎, MMPs, TIMP

Abstract: Objective: To study the mechanism of combination diclofenac with different concentrations of acetylsalicylic acid in rabbit osteoarthritis models. Methods: Rabbit osteoarthritis models were treated with diclofenac acetyl in accordance with different concentrations of alicylic acid. We measured the scores in articular cartilage.PCR and WB were performed to detect the expressions of MMP-3 and TIMP-1.ELASA was used to compare the NO and IL-1β in synovial fluid. Results: Different concentrations of the drug had good therapeutic effect on osteoarthritis of rabbit knee,reduced the scores and the contents of NO and IL-1β,WB method showed the expressions of MMP-3 and MMP-13 protein were down regulated,PCR found upregulation mRNA expression of TIMP-1. Conclusion: Combination of diclofenac with different concentrations of acetylsalicylic acid can relieve the pathological changes in our rabbit osteoarthritis models.The effective therapeutic regimen for osteoarthritis can be verified.The therapeutic goal for osteoarthritis models is achieved by the inhibition of NO, IL-1,MMP-3 and MMP-13 expression, accompanied by the uprugulation of TIMP-1 expression.

Key words: acetylsalicylic acid combined with diclofenac, osteoarthritis, MMPs, TIMP

中图分类号:

R684

侯圣贵, 刘建强. 乙酰水杨酸联合双氯芬酸治疗骨关节炎的机制[J]. betway必威登陆网址（betway.com ）学报, 2021, 42(6): 406-410.

Hou Shenggui, Liu Jianqiang. Mechanism of acetylsalicylic acid combined with diclofenac in the treatment of osteoarthritis[J]. Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences, 2021, 42(6): 406-410.

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乙酰水杨酸联合双氯芬酸治疗骨关节炎的机制

Mechanism of acetylsalicylic acid combined with diclofenac in the treatment of osteoarthritis

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