自拟补气活血方治疗气虚血瘀模型大鼠的血液学评价

doi:10.3969/j.issn.2097-0005.2021.06.003

betway必威登陆网址（betway.com ）学报 ›› 2021, Vol. 42 ›› Issue (6): 411-414.DOI: 10.3969/j.issn.2097-0005.2021.06.003

自拟补气活血方治疗气虚血瘀模型大鼠的血液学评价

许家伟

辽宁中医药大学,辽宁沈阳 110847

收稿日期:2020-12-16 出版日期:2021-06-25 发布日期:2021-08-25
作者简介:许家伟(1995—),男,河南郑州人,辽宁中医药大学硕士在读。E-mail:zhh45690084@163.com。

Hematological evaluation of self-designed prescription for invigorating Qi and activating blood for treating Qi deficiency and blood stasis model rats

Xu Jiawei

Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China

Received:2020-12-16 Online:2021-06-25 Published:2021-08-25

摘要/Abstract

摘要： 目的观察不同剂量补气活血方影响下气虚血瘀模型大鼠的体重及血液学变化情况。方法选择SPF级SD大鼠180只,随机分组,每组30只,采用饥饿、劳累、寒冷的复合刺激方法复制气虚血瘀模型,其中,空白组不造模,正常进食进水,阳性组灌胃补阳还五汤(1.45 g/ml),补气活血方组:以10 ml/kg的剂量灌胃补气活血方,高、中、低三个组的药液浓度分别为1.25 g/ml、0.63 g/ml及0.31 g/ml。分别在造模20天、40天、60天后对模型大鼠进行半自动血液流变仪测血液流变学指标;全自动血凝仪测凝血四项;ELISA法测定血浆中PIVKA-Ⅱ、γ-谷氨酸羧化酶、F1+2片段含量。结果高中低剂量的补气活血方在60天显著缩短APTT和PT(P<0.05),延长FIB(P<0.05);显著上调高中低切变率下大鼠全血粘度(P<0.05);血浆中PIVKA-Ⅱ含量显著下降(P<0.05),谷氨酸羧化酶和F1+2片段含量显著上升(P<0.05)。结论补气活血方具有改善大鼠血液黏度及凝血功能的作用,改善肝脏功能,对低凝状态下气虚血瘀症具有很好的疗效。

关键词: 气虚血瘀, 补气活血方, 组织血流量, 血液流变学

Abstract: Objective: To observe the changes of body weight and hematology in the model rats with Qi deficiency and blood stasis under the influence of different doses of Buqi Huoxue Prescription. Methods: 180 SPF SD rats were selected and randomly divided into groups with 30 rats in each group.The model of Qi deficiency and blood stasis was duplicated by the combined stimulation method of hunger, fatigue and cold. The rats in the blank group were fed with normal water, while the rats in the positive group were given Buyang Huanwu Decoction (1.45g/ml), Buqi Huoxue Decoction group: rats were given Buqi Huoxue Decoction by gavage at the dose of 10ml/kg. The concentration of Buqi Huoxue Decoction in high, medium and low groups were 1.25g/ml, 0.63g/ml and 0.31g/ml respectively.The hemorheology indexes of the model rats were measured by semi-automatic hemorheology after 20 days, 40 days and 60 days of modeling.Four items of coagulation were measured by automatic blood coagulation analyzer.ELISA method was used in the determination of plasma PIVKA Ⅱ, gamma - glutamate carboxylase and F1 + 2 fragment content. Results: The APTT and PT (P＜0.05) were significantly shortened by high, medium and low doses of fuqi and Huoxue Decoction at 60 days.extended FIB(P＜0.05); The whole blood viscosity of rats with high, medium and low shear rate was significantly increased (P＜0.05;PIVKA - Ⅱ content decreased significantly in plasma (P＜0.05), the contents of glutamate carboxylase and F1+2 fragment increased significantly (P＜0.05). Conclusion: The prescription for invigorating Qi and activating blood circulation can improve the blood viscosity and coagulation function of rats and improve liver function, which has a good effect on hypocoagulation and blood stasis.

Key words: Qi deficiency blood stasis, prescription for invigorating Qi and activating blood circulation, tissue blood flow, hemorheology

中图分类号:

R289

许家伟. 自拟补气活血方治疗气虚血瘀模型大鼠的血液学评价[J]. betway必威登陆网址（betway.com ）学报, 2021, 42(6): 411-414.

Xu Jiawei. Hematological evaluation of self-designed prescription for invigorating Qi and activating blood for treating Qi deficiency and blood stasis model rats[J]. Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences, 2021, 42(6): 411-414.

参考文献

[1] 陈雪娇,张哲,王建华,等.基于临床流行病学调查心脑合病气虚血瘀证的发病原因[J].中华中医药学刊,2013,31(10):2120-2123.
[2] 单峰,黄璐琦,郭娟,等.药食同源的历史和发展概况[J].生命科学,2015,27(08):1061-1069.
[3] 李伟霞,唐于平,郭建明,等.比较评价当归川芎配伍对急性血瘀大鼠血液流变学及凝血功能的影响[J].中国中西医结合杂志,2012,32(06):806-811.
[4] 刘凯,王本军,马恒,等.补中益气汤对胃癌术后气虚血瘀证胃肠功能恢复和营养状况的影响[J].中国实验方剂学杂志,2015,21(24):152-156.
[5] 彭康,孙忠.补阳还五汤对中风后遗症“气虚血瘀”大鼠脑组织三磷酸腺苷及二磷酸腺苷和单胺类神经递质的影响[J].中国临床康复,2004(10):1908-1911.
[6] 张颖智, 路娟, 宋婷, 等.加生化胶囊对气虚血瘀模型大鼠血液流变学和细胞内皮因子的影响[J].中成药,2013,35(7):1356-1359.
[7] 庞树玲, 高金亮.中年大鼠气虚血瘀证模拟及其机制探讨[J].天津中医学院学报,1997,16(3):28-31.
[8] 胡耀东. 瑞舒伐他汀对心肌梗死大鼠心室重塑和心功能的影响及其机制[D].中南大学,2010.
[9] 娄金丽,张允岭,郑宏,等.气虚血瘀证动物模型研究的思路与方法[J].北京中医药大学学报,2006(02):87-90.
[10] 王键, 赵辉, 李净,等.多因素复合制作气虚血瘀证脑缺血动物模型的实验研究[J].中国实验动物学报,2001,(42):160-220.
[11] 陈可冀.关于传统血瘀证的现代分析[J].中国中西医结合杂志,2000,20(7):487.
[12] 李雅君,程嘉艺.纳豆+甘麦大枣加味汤对气虚血瘀证大鼠血液流变学和凝血功能的影响[J].长春中医药大学学报,2018,34(01):13-16.
[13] 冠心病合理用药指南[J].中国医学前沿杂志(电子版),2016,8(06):19-108.
[14] 万鹏. 即时凝血功能监测在弥散性血管内凝血患者中的应用评价[D].南方医科大学,2014.
[15] 杨学颖,赵培佐,陈杰鹏,等.维生素K₂对心血管疾病作用的研究进展[J].中国微生态学杂志,2019,31(09):1110-1116.
[16] 李卓江,EberhardF.Mammen.凝血酶原片段F_(1+2)的检测及其临床意义[J].贵州医药,1997(05):257-259+320.

自拟补气活血方治疗气虚血瘀模型大鼠的血液学评价

Hematological evaluation of self-designed prescription for invigorating Qi and activating blood for treating Qi deficiency and blood stasis model rats

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