EGFR/TGF-α/P-AKT/PTEN联合检测在非小细胞肺癌中的临床意义及相关性

doi:10.3969/j.issn.1004-7115.2021.03.006

betway必威登陆网址（betway.com ）学报 ›› 2021, Vol. 42 ›› Issue (3): 185-189.DOI: 10.3969/j.issn.1004-7115.2021.03.006

EGFR/TGF-α/P-AKT/PTEN联合检测在非小细胞肺癌中的临床意义及相关性

袁莉, 李素霞, 王卫华

菏泽医学专科学校药学与检验系,山东菏泽 274000

收稿日期:2020-11-09 出版日期:2021-03-25 发布日期:2021-09-01
作者简介:袁莉,女,硕士,副教授,主要从事肿瘤靶向治疗的研究工作,E-mail:2169825325@qq.com。

Clinical significance of the combined EGFR/TGF-α/P-AKT/PTEN detection to non-small cell lung cancer and their correlations

YUAN Li, LI Su-xia, WANG Wei-hua

Dept. of Pharmacy and Laboratory Medicine, Heze Medical College, Heze 274000, China

Received:2020-11-09 Online:2021-03-25 Published:2021-09-01

摘要/Abstract

摘要： 目的探讨非小细胞肺癌(non-small cell lung carcinoma, NSCLC)中EGFR、TGF-α和P-AKT蛋白与PTEN基因的表达及其与临床病理特征的关系。方法 EGFR、TGF-α和P-AKT蛋白采用免疫组化法、PTEN基因采用原位杂交技术分别检测在NSCLC和正常肺组织中的表达,同时分析各因子间的相关性及其与临床病理特征的关系。结果在NSCLC和正常肺组织中,EGFR的阳性率分别为68.5%(50/73)、30.0%(3/10),P-AKT的阳性率分别为76.7%(56/73)、30.0%(3/10),TGF-α的阳性率分别为72.6%(53/73)、20.0%(2/10),PTEN的阳性率分别为30.1%(22/73)、80.0%(8/10),差异有统计学意义(P＜0.05);EGFR、TGF-α和P-AKT蛋白与PTEN基因的表达分别与淋巴结转移、远处转移和分化程度有关,差异有统计学意义(P＜0.05);EGFR、TGF-α和P-AKT相互之间呈正相关,三者与PTEN呈负相关,差异有统计学意义(P＜0.05)。结论 EGFR、TGF-α和P-AKT的高表达以及PTEN基因缺失在NSCLC的生长增殖和侵袭转移中发挥着重要作用,可以作为NSCLC的检测和预后指标以及靶向治疗药物的研究靶点。

关键词: EGFR, TGF-α, P-AKT, PTEN, 非小细胞肺癌

Abstract: Objective: To probe into the expression of EGFR, TGF-α, P-AKT proteins and PTEN gene in non-small cell lung carcinoma (NSCLC) and their correlations with clinico-pathological characteristics. Methods: Immunohistochemical methods were adopted to detect the expression of EGFR, TGF-α and P-AKT proteins in NSCLC and in situ hybrid technology was used to detect the expression of PTEN gene in NSCLC and normal lung tissues. Analysis was made of correlations between various factors and their relations with clinico-pathological characteristics. Results: In NSCLC and normal lung tissues, the positive rates of EGFR were 68.5%(50/73)and 30.0%(3/10)respectively; the positive rates of P-AKT were 76.7%(56/73)and 30.0%(3/10); the positive rates of TGF-α were 72.6%(53/73)and 20.0%(2/10); the positive rates of PTEN were 30.1%(22/73)and 80.0%(8/10). There was statistically significant difference (P＜0.05). The expressions of EGFR, TGF-α, P-AKT proteins and PTEN gene were respectively related to lymphatic metastasis, distant metastasis and differentiation degree. There was statistically significant difference (P＜0.05). EGFR, TGF-α and P-AKT were positively correlated; however, they were negatively correlated with PTEN gene. The difference was statistically significant(P＜0.05). Conclusion: The overexpression of EGFR, TGF-α and P-AKT and the PTEN gene deletion play a significant role in the growth, proliferation and metastasis of NSCLC. The research findings can serve as a research orientation for NSCLC detection and prognostic indictor as well as for targeted therapeutic drugs.

Key words: EGFR, TGF-α, P-AKT, PTEN, non-small cell lung carcinoma

中图分类号:

R734.2

袁莉, 李素霞, 王卫华. EGFR/TGF-α/P-AKT/PTEN联合检测在非小细胞肺癌中的临床意义及相关性[J]. betway必威登陆网址（betway.com ）学报, 2021, 42(3): 185-189.

YUAN Li, LI Su-xia, WANG Wei-hua. Clinical significance of the combined EGFR/TGF-α/P-AKT/PTEN detection to non-small cell lung cancer and their correlations[J]. Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences, 2021, 42(3): 185-189.

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EGFR/TGF-α/P-AKT/PTEN联合检测在非小细胞肺癌中的临床意义及相关性

Clinical significance of the combined EGFR/TGF-α/P-AKT/PTEN detection to non-small cell lung cancer and their correlations

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