关键词:癌,非小细胞肺,奥曲肽,药物协同作用,Isobologram

Abstract:Objective To investigate whether octreotide, as somatostatin analogue, can enhance the sensitivity of the human lung adenocarcinoma cell line A549 to chemotherapeutic drugs. Methods Different concentration of octretide, cisplatin and 5-Fluorouracil (5-Fu) was respectively acted on the lung adenocarcinoma cell line A549. The absorbance value was tested by colorimetry through MTT method to evaluate the effect of octreotide, cisplatin, 5-Fu or the three drugs combined respectively after 48 hours. Each drug concentration had six holes and it repeated three times. The effects of combination therapy was analysed with isobologram. Results It was proved that octreotide could inhibit the proliferation of A549 cells in a dose-dependent manner at the concentration range of 1.3 mg/L～166.7 mg/L. The inhibition rate was dose-dependent which was higher when octreotide combined with cisplatin and 5-Fu than it alone. It has statistically significant difference (P<0.05).The effect plots of IC₅₀were located in the synergy areas of isobologram. Conclusion It can be concluded that octreotide could inhibit the proliferation of A549 cells in vitro. This inhibition enhances when octreotide is combined with cisplatin and 5-Fu. Octreotide can enhance the susceptibility of A549 cells to cisplatin and 5-Fu.

Key words:Carcinoma, non-small cell lung,Octreotide,Drug synergy,Isobologram