Expressions of HSP90α and HSP90β in colorectal cancer tissues and their clinical significances

Abstract

Abstract:

ObjectiveTo study the expressions of heat shock protein （HSP）90α and HSP90β in colorectal cancer and paracancer tissues, and to investigate the relationships between HSP90α, HSP90β and clinicopathological features of colorectal cancer patients, and to analyze their correlation.MethodsThe tumor tissues and paracancer tissues of 117 patients with colorectal cancer were selected from the Department of Gastrointestinal Surgery, Third Affiliated Hospital of Shandong First Medical University from January 2016 to December 2020. The expression levels of HSP90α and HSP90β were detected by immunohistochemistry, and the relationships between the two proteins and clinicopathological features and the correlation of their expressions were analyzed.ResultsThe positive expression rates of HSP90α in colorectal cancer tissues and paracancer tissues were 74.4% （87/117） and 12.0% （14/117）, and there was a statistically significant difference （χ²=92.83,P<0.001）. The positive expression rate of HSP90β in colorectal cancer tissues and paracancer tissues was 61.5% （72/117） and 10.3% （12/117）, and there was a statistically significant difference （χ²=66.86,P<0.001）. The expression of HSP90α was correlated with tumor location （χ²=8.67,P=0.003）, vascular invasion （χ²=8.68,P=0.003）, lymph node metastasis （χ²=8.52,P=0.004）, T stage （χ²=21.07,P<0.001）, N stage （χ²=11.94,P=0.003）, M stage （χ²=5.37,P=0.020）, pathological stage （χ²=25.64,P<0.001）. The expression of HSP90β was correlated with lymph node metastasis （χ²=4.03,P=0.045）, T stage （χ²=11.09,P=0.007）, N stage （χ²=6.56,P=0.038）, M stage （χ²=12.43,P<0.001）, pathological stage （χ²=17.34,P=0.001）. There was a positive correlation between the expressions of the two proteins in colorectal cancer tissues （r=0.42,P<0.001）.ConclusionThe expressions of HSP90α and HSP90β in colorectal cancer tissues are significantly higher than those in paracancer tissues, and they are related to lymph node metastasis and pathological stage. There is a positive correlation between the two proteins, which may be involved in the occurrence and development of colorectal cancer and are expected to become new tumor markers.

Key words:Colorectal neoplasms,HSP90α,HSP90β

Chen Cunbao, Lu Shoutang, Wang Ruogu, Yang Jianshu, Li Jianqi, Zhen Yanan, Xu Zhongfa. Expressions of HSP90α and HSP90β in colorectal cancer tissues and their clinical significances[J]. Journal of International Oncology, 2022, 49(5): 282-284.

Figures/Tables3

临床病理特征	例数	HSP90α 阳性	χ²值	P值	HSP90β 阳性	χ²值	P值
性别
男	66	49（74.2）	<0.01	0.974	40（60.6）	0.05	0.814
女	51	38（74.5）	<0.01	0.974	32（62.7）	0.05	0.814
年龄（岁）
<65	72	53(73.6）	0.06	0.815	43（59.7）	0.26	0.610
≥65	45	34（75.6）	0.06	0.815	29（64.4）	0.26	0.610
吸烟饮酒史
有	52	41（78.8）	0.99	0.320	34（65.4）	0.58	0.444
无	65	46（70.8）	0.99	0.320	38（58.5）	0.58	0.444
肿瘤位置
结肠	46	41（89.1）	8.67	0.003	31（67.4）	1.09	0.295
直肠	71	46（64.8）	8.67	0.003	41（57.7）	1.09	0.295
血管侵犯
有	32	30（93.8）	8.68	0.003	25（78.1）	2.43	0.119
无	85	57（67.1）	8.68	0.003	47（55.3）	2.43	0.119
神经侵犯
有	13	12（92.3）	2.47	0.116	9（69.2）	0.36	0.545
无	104	75（72.1）	2.47	0.116	63（60.6）	0.36	0.545
淋巴结转移
有	50	44（88.0）	8.52	0.004	36（72.0）	4.03	0.045
无	67	43（64.2）	8.52	0.004	36（53.7）	4.03	0.045
分化程度
高分化	0	0（0）	3.23	0.072	0（0）	1.03	0.369
中分化	105	75（71.4）			63（60.0）
低分化	12	12（100）			9（75.0）
T分期
T₁	4	1（25.0）	21.07	<0.001	1（25.0）	11.09	0.007
T₂	12	3（25.0）			4（33.3）
T₃	16	12（75.0）			7（43.8）
T₄	85	71（83.5）			60（70.6）
N分期
N₀	67	42（62.7）	11.94	0.003	35（52.2）	6.56	0.038
N₁	23	21（91.3）			15（65.2）
N₂	27	24（88.9）			22（81.5）
M分期
M₀	100	70（70.0）	5.37	0.020	55（55.0）	12.43	<0.001
M₁	17	17（100）	5.37	0.020	17（100）	12.43	<0.001
病理分期
Ⅰ	15	4（26.7）	25.64	<0.001	5（33.3）	17.34	0.001
Ⅱ	47	33（70.2）			25（53.2）
Ⅲ	38	33（86.8）			25（65.8）
Ⅳ	17	17（100）			17（100）

HSP90α	HSP90β	合计	r值	P值
阴性	23	7	30	0.42	<0.001
阳性	22	65	87
合计	45	72	117

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