Diagnostic value of detection of serum G-17，sB7-H3，and DKK1 for early gastric cancer

Abstract

Abstract:

ObjectiveTo explore the diagnostic value of detection of serum gastrin-17 (G-17),soluble B7-H3 (sB7-H3),and Dickkopf-1 (DKK1) in early gastric cancer (EGC).MethodsA total of 95 patients with EGC admitted to the Hospital of 81st Group Army of the Chinese People's Liberation Army from April 2021 to April 2023 were selected as the EGC group,95 gastritis patients as the gastritis group,and 95 healthy individuals as the control group. The serum levels of G-17,sB7-H3,and DKK1 were compared among the three groups of subjects,and serum G-17,sB7-H3 and DKK1 levels of patients with different pathological characteristics in EGC group were also compared. Receiver operator characteristic (ROC) curve was applied to analyze the diagnostic value of serum G-17,sB7-H3,and DKK1 levels for EGC. Multivariate logistic regression was applied to analyze the risk factors of EGC.ResultsThe proportions ofHelicobacter pyloriinfection in the control group,gastritis group,and EGC group were 20.00% (19/95),35.79% (34/95),and 61.05% (58/95),respectively,with a statistically significant difference (χ²=34.26,P<0.001). The serum G-17 levels were (6.98±0.55),(8.39±0.95),and (9.46±1.38) pmol/L,respectively,with a statistically significant difference (F=141.82,P<0.001). The levels of sB7-H3 were (18.86±1.64),(20.34±2.16),(22.44±2.62) ng/ml,respectively,with a statistically significant difference (F=64.86,P<0.001). The levels of DKK1 were (12.87±1.96),(14.75±2.09),(6.93±2.24) ng/ml,respectively,with a statistically significant difference (F=88.95,P<0.001). Pairwise comparison showed that the proportion ofHelicobacter pyloriinfection in the control group,gastritis group and EGC group increased in turn,and the levels of serum G-17,sB7-H3 and DKK1 increased in turn (allP<0.05). There were no significant difference in age and gender between EGC patients with different serum G-17,sB7-H3 and DKK1 levels (allP>0.05),and there were statistically significant differences in tumor diameter (χ²=5.74,P=0.017;χ²=4.98,P=0.026;χ²=5.74,P=0.017),tumor invasion (χ²=10.94,P=0.001;χ²=16.16,P<0.001;χ²=21.16,P<0.001),lymph node metastasis (χ²=6.75,P=0.009;χ²=10.68,P=0.001;χ²=4.74,P=0.029) and differentiation type (χ²=7.00,P=0.008;χ²=4.26,P=0.039;χ²=27.18,P<0.001). ROC curve analysis showed that the area under the curve (AUC) of G-17,sB7-H3 and DKK1 in the diagnosis of EGC was 0.825,0.763 and 0.785,respectively. The AUC of the combined detection of the three indicators was 0.933,which was better than that of the three separate tests (Z=3.22,P=0.001;Z=4.94,P<0.001;Z=4.53,P<0.001). Multivariate logistic regression analysis showed that the levels of G-17(OR=3.82,95%CI: 1.73-8.44,P<0.001), sB7-H3(OR=3.96, 95%CI:1.75-8.96,P<0.001), DKK1(OR=3.85, 95%CI:1.77-8.41,P<0.001) were the independent risk factors of EGC.ConclusionSerum G-17,sB7-H3,and DKK1 have certain auxiliary diagnostic value of three indexes for EGC,and the combined detection value is higher.

Key words:Stomach neoplasms,Gastrins,Soluble B7-H3,Dickkopf-1,Diagnosis

Liu Wenhui, Yin Ping, Qi Jie. Diagnostic value of detection of serum G-17，sB7-H3，and DKK1 for early gastric cancer[J]. Journal of International Oncology, 2024, 51(8): 498-503.

Figures/Tables4

指标	对照组（n=95）	胃炎组（n=95）	EGC组（n=95）	F/χ²值	P值
年龄（岁）	56.44±9.28	56.53±9.65	56.88±9.37	0.06	0.944
性别
男	32（33.68）	31（32.63）	30（31.58）	0.10	0.953
女	63（66.32）	64（67.37）	65（68.42）	0.10	0.953
吸烟史
有	38（40.00）	35（36.84）	36（37.89）	0.21	0.901
无	57（60.00）	60（63.16）	59（62.11）	0.21	0.901
饮酒史
有	46（48.42）	42（44.21）	45（47.37）	0.37	0.833
无	49（51.58）	53（55.79）	50（52.63）	0.37	0.833
幽门螺杆菌感染
有	19（20.00）	34（35.79）^a	58（61.05）^ab	34.26	<0.001
无	76（80.00）	61（64.21）^a	37（38.95）^ab	34.26	<0.001
G-17（pmol/L）	6.98±0.55	8.39±0.95^a	9.46±1.38^ab	141.82	<0.001
sB7-H3（ng/ml）	18.86±1.64	20.34±2.16^a	22.44±2.62^ab	64.86	<0.001
DKK1（ng/ml）	12.87±1.96	14.75±2.09^a	16.93±2.24^ab	88.95	<0.001

临床病理特征	例数	G-17				sB7-H3				DKK1
临床病理特征	例数	高水平组（n=48）	低水平组（n=47）	χ²值	P值	高水平组（n=47）	低水平组（n=48）	χ²值	P值	高水平组（n=48）	低水平组（n=47）	χ²值	P值
年龄（岁）
≤60	44	23（52.27）	21（47.73）	0.10	0.752	20（45.45）	24（54.55）	0.53	0.467	26（59.09）	18（40.91）	2.41	0.121
>60	51	25（49.02）	26（50.98）	0.10	0.752	27（52.94）	24（47.06）	0.53	0.467	22（43.14）	29（56.86）	2.41	0.121
性别
男	30	18（60.00）	12（40.00）	1.57	0.210	19（63.33）	11（36.67）	3.37	0.066	16（53.33）	14（46.67）	0.14	0.710
女	65	30（46.15）	35（53.85）	1.57	0.210	28（43.08）	37（56.92）	3.37	0.066	32（49.23）	33（50.77）	0.14	0.710
肿瘤最大径（cm）
≥5	58	35（60.34）	23（39.66）	5.74	0.017	34（58.62）	24（41.38）	4.98	0.026	35（60.34）	23（39.66）	5.74	0.017
<5	37	13（35.14）	24（64.86）	5.74	0.017	13（35.14）	24（64.86）	4.98	0.026	13（35.14）	24（64.86）	5.74	0.017
肿瘤浸润
有	62	39（62.90）	23（37.10）	10.94	0.001	40（64.52）	22（35.48）	16.16	<0.001	42（67.74）	20（32.26）	21.16	<0.001
无	33	9（27.27）	24（72.73）	10.94	0.001	7（21.21）	26（78.79）	16.16	<0.001	6（18.18）	27（81.82）	21.16	<0.001
淋巴结转移
有	57	35（61.40）	22（38.60）	6.75	0.009	36（63.16）	21（36.84）	10.68	0.001	34（59.65）	23（40.35）	4.74	0.029
无	38	13（34.21）	25（65.79）	6.75	0.009	11（28.95）	27（71.05）	10.68	0.001	14（36.84）	24（63.16）	4.74	0.029
分化类型
低/中分化	61	37（60.66）	24（39.34）	7.00	0.008	35（57.38）	26（42.62）	4.26	0.039	43（70.49）	18（29.51）	27.18	<0.001
高分化	34	11（32.35）	23（67.65）	7.00	0.008	12（35.29）	22（64.71）	4.26	0.039	5（14.71）	29（85.29）	27.18	<0.001

变量	β值	SE值	Waldχ²值	OR值	95%CI	P值
幽门螺杆菌感染	0.89	0.47	3.62	2.43	0.97~6.05	0.057
G-17	1.33	0.41	10.93	3.82	1.73~8.44	<0.001
sB7-H3	1.38	0.42	10.87	3.96	1.75~8.96	<0.001
DKK1	1.35	0.40	11.49	3.85	1.77~8.41	<0.001

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