Abstract:Objective To screen phage clones which can specific binding to MDA-MB-231 breast cancer cells from phage random 12 peptide library by phage display technology. Methods Agminated positive monoclonal phage was selected from phage random 12 peptide library, using normal mammary gland cells as subtract screening cells and MDA-MB-231 breast cancer cells as target cells. The specificity and affinity of the positive clones were identified by enzyme linked immunosorbent assay (ELISA) and DAB dyeing. Results Eleven clones were chosen after 3 rounds of screening. ELISA showed the affinity to breast cancer cells of NO.8 phage (named LK-8) was 6.5 times higher than that in control group, immunohistochemistry also showed NO.8 phage had a high specificity bonding to breast cancer cells. Conclusion A specific phage (LK-8) bonding to MDA-MB-231 breast cancer cells is screened successfully using phage display technology, and it lays the foundation for compounding specific polypeptide to diagnose and treat breast cancer.

Key words:Breast neoplasms,Enzyme linked immunosorbent assay,Biological markers,Phage display