Expression and significance of TTF1 and CgA in small cell lung carcinoma

Abstract

Abstract:ObjectiveTo investigate the expression and clinical significance of thyroid transcription factor1 (TTF1) and chromogranin A (CgA) in small cell lung cancer (SCLC). MethodsThe expressions of TTF1 and CgA protein in 68 cases of SCLC tissues and 20 cases of normal lung tissues were examined by immunohistochemistry method, and their correlations with clinical features of SCLC were analyzed. ResultsThe positive rates of TTF1 and CgA protein in SCLC were 88.2% (60/68) and 70.6% (48/68), respectively, and they were higher than those in normal lung tissue ［10.0%(2/20) and 5.0%(1/20); χ2=45.442, P=0.000; χ2=26.941, P=0.000］. The expression of TTF1 protein was not related to the patients′ age, sex and tumor size, while closely related to smoking index (χ2=4.131, P=0.042), lymph node metastasis (χ2=5.488, P=0.019) and clinical stage (χ2=6.011, P=0.014). The expression of CgA protein was not related to the patients′ age, sex, tumor size and smoking index, while closely related to lymph node metastasis (χ2=9.895, P=0.002) and clinical stage (χ2=4.145, P=0.042). ConclusionTTF1 and CgA protein are highly expressed in SCLC, especially in the patients with lymph node metastasis and extensive disease.

Key words:Carcinoma, small cell,Chromogranin A,Immunohistochemistry,Thyroid transcription factor-1

Hu Siqin, Zhang Minghui, Shi Qiong, Wang Yan. Expression and significance of TTF1 and CgA in small cell lung carcinoma[J]. Journal of International Oncology, 2015, 42(7): 501-503.

References

［1］ Siegel R, Ma J, Zou Z, et al. Cancer Statistics, 2014［J］. CA Cancer J Clin, 2014, 64(1): 9-29. ［2］ Lally BE, Urbanic JJ, Blackstock AW, et al. Small cell lung cancer: have we made any progress over the last 25 years？［J］. Oncologist, 2007, 12(9): 1096-1104. ［3］ Bobos M, Hytiroglou P, Kostopoulos I, et al. Immunohistochemical distinction between merkel cell carcinoma and small cell carcinoma of the lung［J］. Am J Dermatopathol, 2006, 28(2): 99-104. ［4］ Kargi A, Gurel D, Tuna B. The diagnostic value of TTF1, CK 5/6, and p63 immunostaining in classification of lung carcinomas［J］. Appl Immunohistochem Mol Morphol, 2007, 15(4): 415-420. ［5］ Drivsholm L, Paloheimo LI, Osterlind K. Chromogranin A, a significant prognostic factor in small cell lung cancer［J］. Br J Cancer, 1999, 81(4): 667-671. ［6］ Molina R, Alvarez E, AnielQuiroga A, et al. Evaluation of chromogranin A determined by three different procedures in patients with benign diseases, neuroendocrine tumors and other malignancies［J］. Tumor Biol, 2011, 32(1): 13-22. ［7］ Petrovi′c M, Bukumiri′c Z, Zdravkovi′c V, et al. The prognostic significance of the circulating neuroendocrine markers chromogranin A, progastrinreleasing peptide, and neuronspecific enolase in patients with smallcell lung cancer［J］. Med Oncol, 2014, 31(2): 823. ［8］周东梅, 孙宇, 李向红. 微管相关蛋白2在240例活检肺小细胞癌中的诊断价值［J］. 中华病理学杂志, 2013, 42(5): 321-324. ［9］ Sayeg Y, Sayeg M, Baum RP, et al. Pulmonary neuroendocrine neoplasms［J］. Pneumologie, 2014, 68(7): 456-477. ［10］ Hamanaka W, Motoi N, Ishikawa S, et al. A subset of small cell lung cancer with low neuroendocrine expression and good prognosis: a comparison study of surgical and inoperable cases with biopsy［J］. Hum Pathol, 2014, 45(5): 1045-1056. ［11］ DettmerM, KimTE, JungCK, et al. Thyroid transcription factor1 expression in colorectal adenocarcinomas［J］. Pathol Res Pract, 2011, 207(11): 686-690. ［12］ Robens J, Goldstein L, Gown AM, et al. Thyroid transcription factor1 expression in breast carcinomas［J］. Am J Surg Pathol, 2010, 34(12): 1881-1885. ［13］林冬梅, 邹霜梅, 吕宁, 等. 甲状腺转录因子1在肺癌中的表达及应用［J］.中华肿瘤杂志, 2004, 26(10): 615-617. ［14］ Hookim K, Kavuri S, Lauer SR, et al. Napsin A expression in small cell carcinoma of the lung: a cytologic study with review of differentials［J］. JASC, 2014, 3(1): 90-95. ［15］ Altaner S, Yoruk Y, Tokatli F, et al. The correlation between TTF1 immunoreactivity and the occurrence of lymph node metastases in patients with lung cancer［J］. Tumori, 2006, 92(4): 323-326. ［16］ Anagnostou VK, Syrigos KN, Bepler G, et al. Thyroid transcription factor 1 is an independent prognostic factor for patients with stage Ⅰ lung adenocarcinoma［J］. J Clin Oncol, 2009, 27(2): 271-278.

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