%A Feng Shu, Wang Junfu, Chen Xuemei, Luan Junwen, Su Qinghong, Luan Meng, Xu Xiaoqun %T Effects of interleukin-17 on human laryngeal carcinoma Hep-2 cells %0 Journal Article %D 2017 %J Journal of International Oncology %R 10.3760/cma.j.issn.1673422X.2017.04.001 %P 241-245 %V 44 %N 4 %U {https://gjzlx.sdfmu.edu.cn/CN/abstract/article_10189.shtml} %8 2017-04-08 %X Objective To investigate the effects of interleukin-17 (IL-17) on the cell proliferation, apoptosis and migration of human laryngeal carcinoma Hep-2 cells. Methods IL-17 was transiently transfected into Hep-2 cells, and at the same time empty vector group (pEGFP-N1) and normal control group were set up. The efficiency of transfection was evaluated by fluorescence microscope, and the mRNA and protein expressions of IL-17 were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. The proliferation of cells was detected by methyl thiazolyl tetrazolium (MTT) method, and the apoptosis was detected by flow cytometry. The migration ability was detected by woundhealing assay and Transwell assay. Results Hep-2 cells transfected with empty vector pEGFP-N1 and IL-17 showed green fluorescence under the fluorescence microscope. Hep-2 cells expressed IL-17 at both mRNA and protein levels after transfection with IL-17. Compared with the normal control group, the proliferation of IL-17 transfected Hep-2 cells was significantly inhibited after 48 h transfection (0.34±0.03 vs. 0.46±0.04, P=0.006). The apoptotic rate of IL-17 transfected cells was higher than that of normal control group (26.80%±0.80% vs. 2.90%±0.31%, P=0.000). According to the woundhealing assay, compared with the normal control group, the scratch width of IL-17 transfected cells was significantly greater (1.59±0.01 vs. 1.36±0.01, P=0.000). Transwell migration experiment showed that the migration of IL-17 transfected cells was significantly lower than that of the normal control group (26.33±2.08 vs. 49.33±1.53, P=0.000). Conclusion IL-17 can inhibit the proliferation of human laryngeal carcinoma Hep-2 cells, reduce their migration ability and enhance their apoptosis ability. Therefore, IL-17 may inhibit the occurrence and development of laryngeal carcinoma through a variety of mechanisms.