N⁶-methyladenine (m⁶A) is the most abundant internal RNA modification found in a variety of eukaryotic messenger RNAs and long non-coding RNAs, which is dynamically regulated by m⁶A methyltransferases and demethylases, and mainly works after recognized by m⁶A binding proteins. m⁶A is involved in almost every step of the RNA life cycle, including RNA transcription, splicing, localization, nuclear transport, translation, and degradation. Disorder of m⁶A modification can lead to RNA dysfunction and abnormal gene expression. m⁶A modification regulators express abnormally in various cancers, and play an important role in cancer formation, proliferation, differentiation, invasion and metastasis.