%A LI Yan-Mei, SONG Guan-Hua, WEN Pei-E, REN Xia, BI Ke-Hong, JIANG Guo-Sheng %T Study on molecular regulation mechanism of VEGF in HL-60 cells after induced differentiation %0 Journal Article %D 2012 %J Journal of International Oncology %R %P 873-877 %V 39 %N 11 %U {https://gjzlx.sdfmu.edu.cn/CN/abstract/article_8936.shtml} %8 2012-11-08 %X Objective To study the molecular regulation mechanism of VEGF in the model of ATRA induced differentiation in HL-60 cells, and to provide new targets for leukemia anti-angiogenic therapy. Methods The morphology was observed by Wright-Gimesa staining; HL-60 cells differentiation was detected by NBT reduction experiment. VEGF,STAT3,c-myc mRNAs were measured by reverse transcription-PCR; VEGF,STAT3 and c-myc proteins were determined by Western blot. Results The proliferation of HL-60 cells was inhibited obviously by ATRA(1 μmol/L) with the induction of differentiation, NBT positive rate was 82.59% ( t=﹣24.157， P <0.01); VEGF mRNA( t=7.339， P＜0.05), STAT3 mRNA ( t=3.667， P＜0.05) and c-myc mRNA（ t=6.858， P＜0.05） were all down-regulated. VEGF protein（ t=3.386， P＜0.05）, STAT3 protein（ t=4.074， P＜0.05）and c-myc protein （ t=3.333， P＜0.05）were all down-regulated. Conclusion VEGF expression level is reduced with the procession of differentiation of HL-60 cells, which may be largely correlated with the down regulation of STAT3 and c-myc.