%A Sima Jin, Zhang Bao, Wang Baojun, Yu Yuanzi, Ai Qing, Zhang Xu %T Numb inhibits proliferation and migration of human bladder cancer cell line BIU87 by negative modulating CXCR4 %0 Journal Article %D 2015 %J Journal of International Oncology %R 10.3760/cma.j.issn.1673-422X.2015.07.004 %P 492-495 %V 42 %N 7 %U {https://gjzlx.sdfmu.edu.cn/CN/abstract/article_9701.shtml} %8 2015-07-08 %X ObjectiveTo study the effect of Numb gene on CXCR4 expression and proliferation and migration of bladder cancer. MethodsHuman bladder cancer cells were divided into 3 groups: experimental group (transfected with NumbORF plasmid), negativecontrol group (transfected with blank vector), and blankcontrol group (no DNA transfected in). The expressions of Numb and CXCR4 were detected by realtime PCR and Western blotting respectively. Cell proliferation and migration were measured by MTS and Transwell assay respectively. ResultsNumb expressions in experimental group, negativecontrol group and blankcontrol group were 31.044±3.350, 4.401±0.567 and 4.287±0.341 respectively, with a statistical significance (F=183.418, P=0.000). CXCR4 levels in experimental group, negativecontrol group and blankcontrol group were 0.344±0.167, 0.996±0.148 and 1.010±0.106 respectively, with a statistical significance (F=21.355, P=0.002). In MTS assay, the absorb values in experimental group, negativecontrol group and blankcontrol group were 0.615±0.057, 0.987±0.063 and 0.957±0.066, with a statistical difference (F=33.210, P=0.001). In Transwell assay, the numbers of migratory cells in experimental group, negativecontrol group and blankcontrol group were 164.667±19.858, 670.133±38.760 and 667.533±27.610, with a statistical significance (F=286.788, P=0.000). ConclusionOverexpression of Numb can suppress the ability of proliferation and migration of the BIU87 cells through downregulation of CXCR4 expression in human bladder cancer.