%A Zhao Xiaolei, Li Sanhua, Liu Lingling, Liang Yongbo, Niu Yinyin, Zhai Jinyu, Xu Yuyin, Qiu Jinkui, Qi Hua %T Detection of ALK gene rearrangement in nonsmall cell lung cancer %0 Journal Article %D 2016 %J Journal of International Oncology %R 10.3760/cma.j.issn.1673-422X.2016.05.005 %P 340-342 %V 43 %N 5 %U {https://gjzlx.sdfmu.edu.cn/CN/abstract/article_9949.shtml} %8 2016-05-08 %X ObjectiveTo detect anaplastic lymphoma kinase (ALK) gene rearrangement in nonsmall cell lung cancer (NSCLC) by fluorescent in situ hybridization (FISH). MethodsAccording to ALK gene rearrangement manners, dualcolor (Red/Green) fluorescence probes were designed and labeled. Specificity and sensitivity were evaluated in peripheral blood metaphase of lymphocyte cells. Test performances were evaluated in NSCLC paraffin tissue samples by FISH and immunohistochemistry. ResultsBoth specificity and sensitivity reached 100% in EpsteinBarr virus (EBV) transformed human lymphocyte cells. The established FISH method detected one ALK gene rearrangement case among two NSCLC samples successfully. The results of FISH were consistent with the results of immunohistochemistry. ConclusionThe dualcolor probe designed in this study can be used for ALK gene rearrangement testing in NSCLC.