2. The First Affiliated Hospital of Shandong First Medical University, Jinan 250014,China"],"fundList_cn":["国家自然科学基金 (81471345)。"],"affList_cn":["1.巨野县人民医院,山东 巨野 274900;
2.betway必威登陆网址 第一附属医院,山东 济南 250014"],"article":{"keywordList_cn":["睡眠剥夺","学习记忆","Orexin-A"],"juan":"42","zhaiyao_cn":"目的 探讨大鼠睡眠剥夺后下丘脑Orexin-A的表达及其对空间学习记忆能力的影响。方法 健康成年雄性Wistar大鼠随机分为空白对照组(CC)、环境对照组(TC)、睡眠剥夺组(SD)。采用间歇性跑步机进行72 h全睡眠剥夺。免疫组化观察各组大鼠下丘脑Orexin-A表达,Morris水迷宫试验测试各组大鼠的空间学习记忆能力。结果 SD组大鼠下丘脑Orexin-A阳性细胞数较TC和CC组显著增加,差异有统计学意义(P<0.01),TC组与CC组相比差异无统计学意义(P＞0.05);与TC组相比,SD组逃避潜伏期明显延长,穿越平台象限的次数明显减少,差异有统计学意义(P＜0.01),TC与CC组比较差异无统计学意义(P＞0.05)。结论 睡眠剥夺可能通过诱导下丘脑Orexin-A的过度表达损害大鼠的空间学习记忆能力。","endNoteUrl_en":"http://xuebao.sdfmu.edu.cn/EN/article/getTxtFile.do?fileType=EndNote&id=33","reference":"[1] Su X, Wang C, Wang X,et al.Sweet dream liquid Chinese Medicine ameliorates learning and memory deficit in a rat model of paradoxical sleep deprivation through the ERK/CREB signaling pathway[J].J Med Food, 2016,19(5):472.
[2] Patrick Y, Lee A, Raha O, et al.Efects of sleep deprivation on cognitive and physical performance in university students[J]. Sleep Biol Rhythms, 2017,15(3):217.
[3] Saadati H, Esmaeili-Mahani S, Esmaeilpour K, et al. Exercise improves learning and memory impairments in sleep deprived female rats[J].Physiol Behav, 2015,138:285.
[4] Mieda M.The roles of orexins in sleep/wake regulation[J]. Neurosci Res, 2017,118:56.
[5] Alzoubi KH, Abdul-Razzak KK, Khabour OF, et al. Caffeine prevents cognitive impairment induced by chronic psychosocial stress and/or high fat-high carbohydrate diet[J]. Behav Brain Res, 2013,237:7.
[6] Riemann D, Spiegelhalder K, Feige B, et al.The hyperarousal model of insomnia: a review of the concept and its evidence[J]. Sleep Med Rev, 2010,14(1):19.
[7] Li G, Tang S, Chi H, et al.Orexin-A aggravates the impairment of hippocampal neurons caused by intermittent hypoxemia by the OXR-PLCβ1-ERK1/2 pathway[J].Neuroreport, 2017,28(6):331.
[8] Guzmán-Marín R, Suntsova N, Stewart DR, et al.Sleep deprivation reduces proliferation of cells in the dentate gyrus of the hippocampus in rats[J].J Physiol, 2003,549(Pt 2):563.
[9] Dubiela FP, Queiroz CM, Moreira KD, et al. AMPA receptors mediate passive avoidance deficits induced by sleep deprivation[J]. Behav Brain Res, 2013,257:189.
[10] Mhaidat NM, Alzoubi KH, Khabour OF, et al.Exploring the effect of vitamin C on sleep deprivation induced memory impairment[J].Brain Res Bull, 2015,113:41.
[11] De Lecea L, Kilduff TS, Peyron C, et al.The hypocretin: hypothalamus-specific peptides with neuroexcitatory activity[J]. Proc Natl Acad Sci USA, 1998,95(1):322.
[12] Postnova S, Voigt K, Braun HA.A mathematical model of homeostatic regulation of sleep-wake cycles by hypocretin/orexin[J]. J Biol Rhythms, 2009,24(6):523.
[13] Tsujino N, Sakurai T.Orexin/hypocretin: a neuropeptide at the interface of sleep, energy homeostasis, and reward system[J]. Pharmacol Rev, 2009,61(2):162.
[14] Yang L, Zou B, Xiong X,et al.Hypocretin/Orexin neurons contribute to hippocampus-dependent social memory and synaptic plasticity in mice[J]. J Neurosci, 2013,33(12):5275.
[15] Lu GL, Lee CH, Chiou LC.Orexin A induces bidirectional modulation of synaptic plasticity: inhibiting long-term potentiation and preventing depotentiation[J].Neuropharmacology,2016,107:168.
[16] Aou S, Li XL, Li AJ, et al. Orexin-A (hypocretin-1) impairs Morris water maze performance and CA1-Schaffer collateral long-term potentiation in rats[J].Neuroscience, 2003,119(4):1221.
[17] Beck B, Pourié G.Ghrelin, neuropeptide Y, and other feeding-regulatory peptides active in the hippocampus: role in learning and memory[J].Nutr Rev, 2013,71(8):541.
[18] Siegel JM, Boehmer LN. Narcolepsy and the hypocretin system-where motion meets emotion[J]. Nat Clin Pract Neurol, 2006,2(10):548.
[19] Lee MG, Hassani OK, Jones BE.Discharge of identified orexin/hypocretin neurons across the sleep-waking cycle[J].J Neurosci, 2005,25(28):6716.
[20] Pedrazzoli M, D'Almeida V, Martins PJ, et al. Increased hypocretin-1 levels in cerebrospinal fluid after REM sleep deprivation[J].Brain Res, 2004,995(1):1.
[21] Longordo F, Kopp C, Luthi A.Consequences of sleep deprivation on neurotransmitter receptor expression and function[J].Eur J Neurosci, 2009,29(9):1810.
[22] Akbari E, Naghdi N, Motamedi F. Functional inactivation of orexin 1 receptors in CA1 region impairs acquisition, consolidation and retrieval in Morris water maze task[J].Behav Brain Res, 2006,173(1):47.
[23] Akbari E, Motamedi F, Naghdi N,et al.The effect of antagonization of orexin 1 receptors in CA1 and dentate gyrusregions on memory processing in passive avoidance task[J].Behav Brain Res, 2008,187(1):172.
[24] Raoof R, Esmaeili-Mahani S, Abbasnejad M, et al. Changes in hippocampal orexin 1 receptor expression involved in tooth pain-induced learning and memory impairment in rats[J]. Neuropeptides, 2015,50:9.
[25] Roecker AJ, Cox CD, Coleman PJ, et al.Orexin receptor antagonists: New therapeutic agents for the treatment of insomnia[J].J Med Chem, 2016,59(2):504.
[26] Uslaner JM, Tye SJ, Eddins DM.Orexin receptor antagonists differ from standard sleep drugs by promoting sleep at doses that do not disrupt cognition[J].Sci Transl Med, 2013,5(179):179ra44.","bibtexUrl_cn":"//www.pitakata.com/xuebao/CN/article/getTxtFile.do?fileType=BibTeX&id=33","abstractUrl_en":"http://xuebao.sdfmu.edu.cn/EN/10.3969/j.issn.2097-0005.2021.05.010","qi":"5","id":33,"nian":2021,"bianHao":"1630374531246-2044718514","zuoZheEn_L":"Ni Liyan, Tang Jiyou","juanUrl_en":"http://xuebao.sdfmu.edu.cn/EN/Y2021","clcIndexList_en":[{"code":"R741.02","text":""}],"shouCiFaBuRiQi":"2021-08-31","qiShiYe":"380","received":"2021-01-25","qiUrl_cn":"http://xuebao.sdfmu.edu.cn/CN/Y2021/V42/I5","lanMu_cn":"基础研究","pdfSize":"6148","zuoZhe_CN":"倪丽艳1, 唐吉友2","risUrl_cn":"//www.pitakata.com/xuebao/CN/article/getTxtFile.do?fileType=Ris&id=33","title_cn":"睡眠剥夺后大鼠下丘脑Orexin-A神经元的表达及其对学习记忆的影响","doi":"10.3969/j.issn.2097-0005.2021.05.010","jieShuYe":"384","keywordList_en":["sleep deprivation","learning and memory","Orexin-A"],"endNoteUrl_cn":"//www.pitakata.com/xuebao/CN/article/getTxtFile.do?fileType=EndNote&id=33","zhaiyao_en":"Objective: To investigate the expression of Orexin-A neurons in the hypothalamus of rats after sleep deprivation and its effect on learning and memory.Methods: Healthy adult male Wistar rats were randomly divided into cage control (CC), treadmill control (TC), sleep deprivation (SD) group. 72h total sleep deprivation was performed by intermittent treadmill. The expression of Orexin-A was observed by immunohistochemistry, and the spatial learning and memory ability was tested by Morris water maze test. Results: Compared with TC, the number of Orexin-A positive cells in hypothalamus of SD rats was significantly increased (P<0.01). There was no significant difference between TC and CC group (P>0.05). Compared with TC group, the escape latency and the times of crossing platform quadrant in SD group were significantly longer (P<0.01), and there was no significant difference between TC group and CC group (P>0.05). Conclusion: Sleep deprivation may impair spatial learning and memory in rats by inducing overexpression of Orexin-A in hypothalamus.","bibtexUrl_en":"http://xuebao.sdfmu.edu.cn/EN/article/getTxtFile.do?fileType=BibTeX&id=33","abstractUrl_cn":"http://xuebao.sdfmu.edu.cn/CN/10.3969/j.issn.2097-0005.2021.05.010","zuoZheCn_L":"倪丽艳, 唐吉友","juanUrl_cn":"http://xuebao.sdfmu.edu.cn/CN/Y2021","lanMu_en":"Basic Researches","clcIndexList_cn":[{"code":"R741.02","text":""}],"qiUrl_en":"//www.pitakata.com/xuebao/EN/Y2021/V42/I5","zuoZhe_EN":"Ni Liyan1, Tang Jiyou2","risUrl_en":"http://xuebao.sdfmu.edu.cn/EN/article/getTxtFile.do?fileType=Ris&id=33","title_en":"The expression of Orexin-A neurons in the hypothalamus of rats after sleep deprivation and its effect on learning and memory","hasPdf":"true"},"authorNotes_cn":["倪丽艳,硕士研究生,主治医师,研究方向:神经病学,E-mail:niliyan122@163.com。"],"authorList_en":[{"deceased":false,"name_cn":"倪丽艳","name_en":"Ni Liyan"},{"deceased":false,"name_cn":" 唐吉友","name_en":" Tang Jiyou"}]}">

The expression of Orexin-A neurons in the hypothalamus of rats after sleep deprivation and its effect on learning and memory

Ni Liyan, Tang Jiyou

Journal of ShanDong First Medical University&ShanDong Academy of Medical Sciences››2021, Vol. 42››Issue (5): 380-384.

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Journal of ShanDong First Medical University&ShanDong Academy of Medical Sciences ›› 2021, Vol. 42 ›› Issue (5) : 380-384. DOI: 10.3969/j.issn.2097-0005.2021.05.010
Basic Researches

The expression of Orexin-A neurons in the hypothalamus of rats after sleep deprivation and its effect on learning and memory

  • Ni Liyan1, Tang Jiyou2
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Abstract

Objective: To investigate the expression of Orexin-A neurons in the hypothalamus of rats after sleep deprivation and its effect on learning and memory.Methods: Healthy adult male Wistar rats were randomly divided into cage control (CC), treadmill control (TC), sleep deprivation (SD) group. 72h total sleep deprivation was performed by intermittent treadmill. The expression of Orexin-A was observed by immunohistochemistry, and the spatial learning and memory ability was tested by Morris water maze test. Results: Compared with TC, the number of Orexin-A positive cells in hypothalamus of SD rats was significantly increased ( P<0.01). There was no significant difference between TC and CC group ( P>0.05). Compared with TC group, the escape latency and the times of crossing platform quadrant in SD group were significantly longer ( P<0.01), and there was no significant difference between TC group and CC group ( P>0.05). Conclusion: Sleep deprivation may impair spatial learning and memory in rats by inducing overexpression of Orexin-A in hypothalamus.

Key words

sleep deprivation/learning and memory/Orexin-A

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Ni Liyan, Tang Jiyou.The expression of Orexin-A neurons in the hypothalamus of rats after sleep deprivation and its effect on learning and memory[J]. Journal of ShanDong First Medical University&ShanDong Academy of Medical Sciences. 2021, 42(5): 380-384 https://doi.org/10.3969/j.issn.2097-0005.2021.05.010

References

[1] Su X, Wang C, Wang X,et al.Sweet dream liquid Chinese Medicine ameliorates learning and memory deficit in a rat model of paradoxical sleep deprivation through the ERK/CREB signaling pathway[J].J Med Food, 2016,19(5):472.
[2] Patrick Y, Lee A, Raha O, et al.Efects of sleep deprivation on cognitive and physical performance in university students[J]. Sleep Biol Rhythms, 2017,15(3):217.
[3] Saadati H, Esmaeili-Mahani S, Esmaeilpour K, et al. Exercise improves learning and memory impairments in sleep deprived female rats[J].Physiol Behav, 2015,138:285.
[4] Mieda M.The roles of orexins in sleep/wake regulation[J]. Neurosci Res, 2017,118:56.
[5] Alzoubi KH, Abdul-Razzak KK, Khabour OF, et al. Caffeine prevents cognitive impairment induced by chronic psychosocial stress and/or high fat-high carbohydrate diet[J]. Behav Brain Res, 2013,237:7.
[6] Riemann D, Spiegelhalder K, Feige B, et al.The hyperarousal model of insomnia: a review of the concept and its evidence[J]. Sleep Med Rev, 2010,14(1):19.
[7] Li G, Tang S, Chi H, et al.Orexin-A aggravates the impairment of hippocampal neurons caused by intermittent hypoxemia by the OXR-PLCβ1-ERK1/2 pathway[J].Neuroreport, 2017,28(6):331.
[8] Guzmán-Marín R, Suntsova N, Stewart DR, et al.Sleep deprivation reduces proliferation of cells in the dentate gyrus of the hippocampus in rats[J].J Physiol, 2003,549(Pt 2):563.
[9] Dubiela FP, Queiroz CM, Moreira KD, et al. AMPA receptors mediate passive avoidance deficits induced by sleep deprivation[J]. Behav Brain Res, 2013,257:189.
[10] Mhaidat NM, Alzoubi KH, Khabour OF, et al.Exploring the effect of vitamin C on sleep deprivation induced memory impairment[J].Brain Res Bull, 2015,113:41.
[11] De Lecea L, Kilduff TS, Peyron C, et al.The hypocretin: hypothalamus-specific peptides with neuroexcitatory activity[J]. Proc Natl Acad Sci USA, 1998,95(1):322.
[12] Postnova S, Voigt K, Braun HA.A mathematical model of homeostatic regulation of sleep-wake cycles by hypocretin/orexin[J]. J Biol Rhythms, 2009,24(6):523.
[13] Tsujino N, Sakurai T.Orexin/hypocretin: a neuropeptide at the interface of sleep, energy homeostasis, and reward system[J]. Pharmacol Rev, 2009,61(2):162.
[14] Yang L, Zou B, Xiong X,et al.Hypocretin/Orexin neurons contribute to hippocampus-dependent social memory and synaptic plasticity in mice[J]. J Neurosci, 2013,33(12):5275.
[15] Lu GL, Lee CH, Chiou LC.Orexin A induces bidirectional modulation of synaptic plasticity: inhibiting long-term potentiation and preventing depotentiation[J].Neuropharmacology,2016,107:168.
[16] Aou S, Li XL, Li AJ, et al. Orexin-A (hypocretin-1) impairs Morris water maze performance and CA1-Schaffer collateral long-term potentiation in rats[J].Neuroscience, 2003,119(4):1221.
[17] Beck B, Pourié G.Ghrelin, neuropeptide Y, and other feeding-regulatory peptides active in the hippocampus: role in learning and memory[J].Nutr Rev, 2013,71(8):541.
[18] Siegel JM, Boehmer LN. Narcolepsy and the hypocretin system-where motion meets emotion[J]. Nat Clin Pract Neurol, 2006,2(10):548.
[19] Lee MG, Hassani OK, Jones BE.Discharge of identified orexin/hypocretin neurons across the sleep-waking cycle[J].J Neurosci, 2005,25(28):6716.
[20] Pedrazzoli M, D'Almeida V, Martins PJ, et al. Increased hypocretin-1 levels in cerebrospinal fluid after REM sleep deprivation[J].Brain Res, 2004,995(1):1.
[21] Longordo F, Kopp C, Luthi A.Consequences of sleep deprivation on neurotransmitter receptor expression and function[J].Eur J Neurosci, 2009,29(9):1810.
[22] Akbari E, Naghdi N, Motamedi F. Functional inactivation of orexin 1 receptors in CA1 region impairs acquisition, consolidation and retrieval in Morris water maze task[J].Behav Brain Res, 2006,173(1):47.
[23] Akbari E, Motamedi F, Naghdi N,et al.The effect of antagonization of orexin 1 receptors in CA1 and dentate gyrusregions on memory processing in passive avoidance task[J].Behav Brain Res, 2008,187(1):172.
[24] Raoof R, Esmaeili-Mahani S, Abbasnejad M, et al. Changes in hippocampal orexin 1 receptor expression involved in tooth pain-induced learning and memory impairment in rats[J]. Neuropeptides, 2015,50:9.
[25] Roecker AJ, Cox CD, Coleman PJ, et al.Orexin receptor antagonists: New therapeutic agents for the treatment of insomnia[J].J Med Chem, 2016,59(2):504.
[26] Uslaner JM, Tye SJ, Eddins DM.Orexin receptor antagonists differ from standard sleep drugs by promoting sleep at doses that do not disrupt cognition[J].Sci Transl Med, 2013,5(179):179ra44.
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