Mechanism of acetylsalicylic acid combined with diclofenac in the treatment of osteoarthritis

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Journal of ShanDong First Medical University&ShanDong Academy of Medical Sciences ›› 2021, Vol. 42 ›› Issue (6) : 406-410. DOI: 10.3969/j.issn.2097-0005.2021.06.002

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Hou Shenggui, Liu Jianqiang

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Received	Published
2020-12-13	2021-06-25
Issue Date
2021-08-25

Abstract

Objective: To study the mechanism of combination diclofenac with different concentrations of acetylsalicylic acid in rabbit osteoarthritis models. Methods: Rabbit osteoarthritis models were treated with diclofenac acetyl in accordance with different concentrations of alicylic acid. We measured the scores in articular cartilage.PCR and WB were performed to detect the expressions of MMP-3 and TIMP-1.ELASA was used to compare the NO and IL-1β in synovial fluid. Results: Different concentrations of the drug had good therapeutic effect on osteoarthritis of rabbit knee,reduced the scores and the contents of NO and IL-1β,WB method showed the expressions of MMP-3 and MMP-13 protein were down regulated,PCR found upregulation mRNA expression of TIMP-1. Conclusion: Combination of diclofenac with different concentrations of acetylsalicylic acid can relieve the pathological changes in our rabbit osteoarthritis models.The effective therapeutic regimen for osteoarthritis can be verified.The therapeutic goal for osteoarthritis models is achieved by the inhibition of NO, IL-1,MMP-3 and MMP-13 expression, accompanied by the uprugulation of TIMP-1 expression.

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acetylsalicylic acid combined with diclofenac/osteoarthritis/MMPs/TIMP

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Hou Shenggui, Liu Jianqiang.Mechanism of acetylsalicylic acid combined with diclofenac in the treatment of osteoarthritis[J]. Journal of ShanDong First Medical University&ShanDong Academy of Medical Sciences. 2021, 42(6): 406-410 https://doi.org/10.3969/j.issn.2097-0005.2021.06.002

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