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探索双花保健口服液的最佳制备工艺并对双花保健口服液对应激性肝损伤的保护作用进行药效学评价。
得到金银花、菊花乙醇提取物，设计正交试验，探索最佳的双花保健口服液制备条件，并通过《中国药典》检测口服液是否符合质量标准。进一步利用拘束负荷的方法建立应激性肝损伤小鼠模型，依次对小鼠血清中谷丙转氨酶（ALT）、小鼠肝组织超氧化物歧化酶（SOD）的活力以及丙二醛（MDA）的含量进行测定，观察双花口服液对小鼠肝脏应激性肝损伤的影响，以评价双花保健口服液对应激性肝损伤的保护作用。
正交试验得出双花口服液最佳的制备工艺为：8%双花浸膏混合物（金银花∶菊花=1∶1），4%蔗糖，0.10%柠檬酸。该工艺制得的口服液符合药典所规定的质量标准。小鼠拘束负荷应激实验结果表明，双花保健口服液对应激性肝损伤有一定的保护作用，能降低ALT水平，升高SOD活力，降低MDA的含量。
正交试验优化出的双花保健口服液的最佳处方条件符合《中国药典》的质量标准，双花保健口服液对小鼠的应激性肝损伤有一定的保护作用。
| 1 | 国家药典委员会.中华人民共和国药典：一部［S］.2005版.北京：中国医药科技出版社， 2005：152. |
| 2 | 高玉敏.浅析金银花的药理作用与临床应用［J］.中医中药，2018，16（35）：164. |
| 3 | 吴娇，王聪，于海川.金银花中的化学成分及其药理作用研究进展［J］.中国实验方剂学杂志， 2019，25（4）：225. |
| 4 | 王东升.金银花提取物对肝损伤小鼠的保护作用研究［J］.医药导报， 2011， 30（8）：1010. |
| 5 | 田磊，蒋宝平，何苗，等.金银花-山银花水提液不同配比体外抗氧化作用研究［J］.实用药物与临床， 2014， 17（10）：1290. |
| 6 | 王存琴，汪荣斌，张艳华.菊花的化学成分及药理活性［J］.长春中医药大学学报， 2014，30（1）：28. |
| 7 | 刘磊磊，肖卓炳. 野菊花的化学成分研究［J］.中草药，2018，49（22）：5254. |
| 8 | 杜闻伟，高善荣，罗崇念，等.菊花提取物对大鼠肝脏脂质过氧化作用的影响［J］. 现代中药研究与实践，2009，23（3）：40. |
| 9 | Kurihara H，Koda H，Asami S， et al. Contribution of the antioxidative property of a staxanthin to its protective effect on the promotion of cancer metastasis in mice treated with restraint stress［J］. Life Sci，2002，70（21）： 2509. |
| 10 | Ｒauen U，Ｒeuters I，Fuchs A， et al. Oxygen-free radical-mediated injury to cultured rat hepatocytes during cold incubation inpreservation solutions［J］.Hepatology，1997，26（2）： 351. |
| 11 | 何蓉蓉，姚新生，栗原博. 拘束应激动物模型的研究现状与应用［J］.中国实验方剂学杂志，2008，14（6）：80. |
| 12 | 朱焕容，黄晓丹，罗燕玉，等.菊花、金银花浸膏的提取工艺研究［J］. 中国医药导报， 2012，9（36）：132. |
| 13 | 王敏，王大平，王文芳，等.保利甘胶囊对拘束负荷引起小鼠肝损伤的保护作用［J］. 中药新药与临床药理，2009，20（5）：412． |
| 14 | 王敏，何蓉蓉，李怡芳，等.五指毛桃水提物对拘束应激性肝损伤的保护作用［J］.中国医院药学杂志，2015，35（6）：522. |
| 15 | 汪艳丽，余燕影，田洁，等.水飞蓟宾乳糖修饰对急性酒精性肝损伤保护作用的影响［J］.时珍国医国药， 2013， 24（10）：2360. |
| 16 | 刘长，华春秀，归改霞，等. 和厚朴酚对小鼠四氯化碳肝损伤的保护作用［J］.郑州大学学报（医学版）， 2013，48（4）： 470. |
| 17 | Adetoro KO，Bolanle JD，Abdullahi SB， et al． In vivo antioxidant effect of aqueous root bark，stem bark and leaves extracts of Vitex doniana in CCl4 induced liver damage rats ［J］.Asian Pac J Trop Biomed，2013， 3（5）： 395. |
| 18 | 周军，张福华. 桑叶黄酮对四氯化碳诱导的小鼠急性肝损伤保护作用［J］.中国实验方剂学杂志. 2013，19（15）： 269. |
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Important active components were extracted from ethanol extract of honeysuckle and chrysanthemum， the best conditions for the preparation of oral liquid oral liquidand suitable accessories were explored by designing the orthogonal experiment， and whether the oral liquid met the quality standards was detected .The effect of honeysuckle and chrysanthemum double flower oral liquid on liver stress induced liver injury in mice was further explored to evaluate the protective effect of the double flower health oral liquid on the induced liver injury. Establishment of stress liver injury mouse model by restraint load method， the activities of ALT in serum and the content of SOD and MDA in liver were determined to evaluate the hepatoprotective effects.
Orthogonal test showed that the best preparation technology of the double flower oral liquid was： 8% the mixture of flower extract （honeysuckle∶chrysanthemum = 1∶1）， 4% sucrose and 0.10% citric acid. The oral liquid prepared by this process accords with the quality standard stipulated in the Pharmacopoeia. The experimental results showed that the double flower health oral liquid had certain protective effects on induced liver injury， reduced the level of ALT， increased the level of SOD and reduced the content of MDA.
Through orthogonal test， the best prescription condition of the double flower health oral liquid is optimized， which accords with the quality standard of Chinese Pharmacopoeia. At the same time， the double flower health oral liquid can protect and improve stress liver injury in mice.
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Preparation of double flower health oral liquid and its protective effect on liver injury induced by stress
Qinxue Jing, Tianwei Wang, Jingyu Wang, Ya′nan Chen, Li Zhang, Xiaoyan Xu, Shaobon Pan
Journal of ShanDong First Medical University&ShanDong Academy of Medical Sciences››2021, Vol. 42››Issue (7): 501-503.
PDF(437 KB)
PDF(437 KB)
Preparation of double flower health oral liquid and its protective effect on liver injury induced by stress
To explore the best preparation process of the double flower health oral liquid and to evaluate the protective effect of the double flower health oral liquid on stress-induced liver injury.
Important active components were extracted from ethanol extract of honeysuckle and chrysanthemum， the best conditions for the preparation of oral liquid oral liquidand suitable accessories were explored by designing the orthogonal experiment， and whether the oral liquid met the quality standards was detected .The effect of honeysuckle and chrysanthemum double flower oral liquid on liver stress induced liver injury in mice was further explored to evaluate the protective effect of the double flower health oral liquid on the induced liver injury. Establishment of stress liver injury mouse model by restraint load method， the activities of ALT in serum and the content of SOD and MDA in liver were determined to evaluate the hepatoprotective effects.
Orthogonal test showed that the best preparation technology of the double flower oral liquid was： 8% the mixture of flower extract （honeysuckle∶chrysanthemum = 1∶1）， 4% sucrose and 0.10% citric acid. The oral liquid prepared by this process accords with the quality standard stipulated in the Pharmacopoeia. The experimental results showed that the double flower health oral liquid had certain protective effects on induced liver injury， reduced the level of ALT， increased the level of SOD and reduced the content of MDA.
Through orthogonal test， the best prescription condition of the double flower health oral liquid is optimized， which accords with the quality standard of Chinese Pharmacopoeia. At the same time， the double flower health oral liquid can protect and improve stress liver injury in mice.
honeysuckle/chrysanthemum/ethanol extract/stress liver injury
| 1 | 国家药典委员会.中华人民共和国药典：一部［S］.2005版.北京：中国医药科技出版社， 2005：152. |
| 2 | 高玉敏.浅析金银花的药理作用与临床应用［J］.中医中药，2018，16（35）：164. |
| 3 | 吴娇，王聪，于海川.金银花中的化学成分及其药理作用研究进展［J］.中国实验方剂学杂志， 2019，25（4）：225. |
| 4 | 王东升.金银花提取物对肝损伤小鼠的保护作用研究［J］.医药导报， 2011， 30（8）：1010. |
| 5 | 田磊，蒋宝平，何苗，等.金银花-山银花水提液不同配比体外抗氧化作用研究［J］.实用药物与临床， 2014， 17（10）：1290. |
| 6 | 王存琴，汪荣斌，张艳华.菊花的化学成分及药理活性［J］.长春中医药大学学报， 2014，30（1）：28. |
| 7 | 刘磊磊，肖卓炳. 野菊花的化学成分研究［J］.中草药，2018，49（22）：5254. |
| 8 | 杜闻伟，高善荣，罗崇念，等.菊花提取物对大鼠肝脏脂质过氧化作用的影响［J］. 现代中药研究与实践，2009，23（3）：40. |
| 9 | Kurihara H，Koda H，Asami S， et al. Contribution of the antioxidative property of a staxanthin to its protective effect on the promotion of cancer metastasis in mice treated with restraint stress［J］. Life Sci，2002，70（21）： 2509. |
| 10 | Ｒauen U，Ｒeuters I，Fuchs A， et al. Oxygen-free radical-mediated injury to cultured rat hepatocytes during cold incubation inpreservation solutions［J］.Hepatology，1997，26（2）： 351. |
| 11 | 何蓉蓉，姚新生，栗原博. 拘束应激动物模型的研究现状与应用［J］.中国实验方剂学杂志，2008，14（6）：80. |
| 12 | 朱焕容，黄晓丹，罗燕玉，等.菊花、金银花浸膏的提取工艺研究［J］. 中国医药导报， 2012，9（36）：132. |
| 13 | 王敏，王大平，王文芳，等.保利甘胶囊对拘束负荷引起小鼠肝损伤的保护作用［J］. 中药新药与临床药理，2009，20（5）：412． |
| 14 | 王敏，何蓉蓉，李怡芳，等.五指毛桃水提物对拘束应激性肝损伤的保护作用［J］.中国医院药学杂志，2015，35（6）：522. |
| 15 | 汪艳丽，余燕影，田洁，等.水飞蓟宾乳糖修饰对急性酒精性肝损伤保护作用的影响［J］.时珍国医国药， 2013， 24（10）：2360. |
| 16 | 刘长，华春秀，归改霞，等. 和厚朴酚对小鼠四氯化碳肝损伤的保护作用［J］.郑州大学学报（医学版）， 2013，48（4）： 470. |
| 17 | Adetoro KO，Bolanle JD，Abdullahi SB， et al． In vivo antioxidant effect of aqueous root bark，stem bark and leaves extracts of Vitex doniana in CCl4 induced liver damage rats ［J］.Asian Pac J Trop Biomed，2013， 3（5）： 395. |
| 18 | 周军，张福华. 桑叶黄酮对四氯化碳诱导的小鼠急性肝损伤保护作用［J］.中国实验方剂学杂志. 2013，19（15）： 269. |
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