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研究GATA5对肾癌HEK-293细胞系增殖调控及对细胞重编程因子EpCAM表达的影响。
构建pTT5-GATA5表达载体并进行基因序列测序验证；四甲基偶氮唑盐（MTT）法检测细胞增殖率；细胞划痕实验检测细胞划痕修复情况；实时定量反转录-聚合酶链反应（RT-PCR）检测基因mRNA表达；蛋白免疫印迹（Western Blot）检测蛋白表达。
GATA5抑制HEK-293细胞增殖；GATA5抑制HEK-293细胞划痕修复；GATA5抑制EpCAM的mRNA和蛋白表达。
GATA5可以抑制肾癌细胞HEK-293细胞的增殖率并抑制EpCAM表达。
| 1 | Liu B，Liu NN，Liu WH，et al. Inhibition of lectin-like oxidized low-density lipoprotein receptor-1 reduces cardiac fibroblast proliferation by suppressing GATA Binding Protein 4［J］. Biochemical and Biophysical Research Communications，2016：S0006291X16308038. |
| 2 | Guo J，Wang ZY，Wu JC， et al. Endothelial SIRT6 Is Vital to Prevent Hypertension and Associated Cardiorenal Injury Through Targeting Nkx3.2-GATA5 Signaling.［J］ .Circ Res， 2019，124： 1448. |
| 3 | Shu J，Zhang K，Zhang M，et al. GATA family members as inducers for cellular reprogramming to pluripotency［J］. Cell Research，2015，25（2）：169. |
| 4 | Song ZP，Chen L，Pang SC， et al. Molecular genetic study on GATA5 gene promoter in acute myocardial infarction.［J］ .PLoS One，2021，16： e0248203. |
| 5 | Akiyama Y，Watkins N，Suzuki H，et al. GATA-4 and GATA-5 transcription factor genes and potential downstream antitumor target genes are epigenetically silenced in colorectal and gastric cancer［J］. Molecular & Cellular Biology，2003，23（23）：8429. |
| 6 | Xia L，Gong Y，Zhang A，et al. Loss of GATA5 expression due to gene promoter methylation induces growth and colony formation of hepatocellular carcinoma cells［J］. Oncology Letters， 2015. |
| 7 | Liu X，Feng Q，Zhang YR，et al. Absence of EpCAM in cervical cancer cells is involved in sluginduced epithelial-mesenchymal transition.［J］ .Cancer Cell Int，2021，21：163. |
| 8 | Song MH，Kim YR，Bae JH，et al. A cancer/testis antigen，NY-SAR-35，induces EpCAM， CD44，and CD133，and activates ERK in HEK293 cells［J］. Biochemical & Biophysical Research Communications，2017，484（2）：298. |
| 9 | Gao Y， Fan WH，Song ZB，et al. Comparison of circulating tumor cell （CTC） detection rates with epithelial cell adhesion molecule （EpCAM） and cell surface vimentin （CSV） antibodies in different solid tumors： a retrospective study［J］ .PeerJ，2021，9： e10777. |
| 10 | Canis M， Lechner A， Mack B， et al. CD133 induces tumour-initiating properties in HEK293 cells［J］. Tumour Biology the Journal of the International Society for Oncodevelopmental Biology & Medicine，2013，34（1）：437. |
| 11 | Shi M，Xie DC，Gaod Y， et al.Targeting miRNAs for pancreatic cancer therapy.［J］ .Curr Pharm Des，2014，20：5279. |
| 12 | Hellebrekers D M E I， Lentjes M H F M，Van d B S M，et al. GATA4 and GATA5 are Potential Tumor Suppressors and Biomarkers in Colorectal Cancer［J］. Clinical Cancer Research，2009， 15（12）：3990. |
| 13 | Amanda O，Aloysius W，Luke E，et al. A Guide to Transient Expression of Membrane Proteins in HEK-293 Cells for Functional Characterization：［J］. Frontiers in Physiology，2016，7：300. |
| 14 | Hu Y，Wu Q，Gao JL，et al. A meta-analysis and The Cancer Genome Atlas data of prostate cancer risk and prognosis using epithelial cell adhesion molecule （EpCAM） expression.［J］ .BMC Urol，2019，19： 67. |
| 15 | Nubel T，Preobraschenski J，Tuncay H，et al. Claudin-7 Regulates EpCAM-Mediated Functions in Tumor Progression［J］. Molecular Cancer Research，2009，7（3）：285. |
| 16 | Lindemans CA，Calafiore M，Mertelsmann AM，et al. Interleukin-22 promotes intestinal-stem-cell-mediated epithelial regeneration.［J］. Nature，2015，528（7583）：560. |
| 17 | Guo M，Akiyama Y，House MG，et al. Hypermethylation of the GATA Genes in Lung Cancer［J］. Clinical Cancer Research An Official Journal of the American Association for Cancer Research，2004，10（23）：7917. |
| 18 | Haworth K E，Kotecha S，Mohun T J，et al. GATA4 and GATA5 are essential for heart and liver development in Xenopus embryos［J］. Bmc Developmental Biology，2008，8（1）：1. |
| 19 | Hellebrekers D M，Lentjes M H，Sm V D B，et al. GATA4 and GATA5 are potential tumor suppressors and biomarkers in colorectal cancer［J］. Clinical Cancer Research，2009，15（12）：3990. |
| 20 | Peters I，Gebauer K，Dubrowinskaja N，et al. GATA5 CpG island hypermethylation is an independent predictor for poor clinical outcome in renal cell carcinoma［J］. Oncology Reports， 2014，31（4）：1523. |
| 21 | Kuan II，Lee CC，Chen CH，et al. The extracellular domain of epithelial cell adhesion molecule （EpCAM） enhances multipotency of mesenchymal stem cells through EGFR-LIN28-LET7 signaling.［J］ .J. Biol. Chem.，2019，294： 7769. |
), 石嵩1, 陈文1, 关建民2()","risUrl_cn":"//www.pitakata.com/xuebao/CN/article/getTxtFile.do?fileType=Ris&id=147","title_cn":"GATA5抑制EpCAM 表达对肾癌HEK-293细胞系的影响","doi":"10.3969/j.issn.2097-0005.2021.08.004","jieShuYe":"583","keywordList_en":["GATA5","EpCAM","HEK-293 cells","renal cancer"],"endNoteUrl_cn":"//www.pitakata.com/xuebao/CN/article/getTxtFile.do?fileType=EndNote&id=147","zhaiyao_en":"To study the effect of GATA5 on the proliferation regulation of renal cell carcinoma HEK-293 cell line and the expression of cell reprogramming factor EpCAM.
Construction of pTT5-GATA5 expression vector and sequence verification of gene sequence； MTT method to detect cell proliferation rate； cell scratch test to detect cell scratch repair； RT-PCR to detect gene mRNA expression； western blot detect protein expression.
GATA5 inhibits HEK-293 cell proliferation； GATA5 inhibits HEK-293 cell scratch repair； GATA5 inhibits EpCAM mRNA and protein expression.
GATA5 can inhibit the proliferation rate of renal cancer cell HEK-293 cells and inhibit the expression of EpCAM.
), Song Shi1, Wen Chen1, Jianmin Guan2()","risUrl_en":"http://xuebao.sdfmu.edu.cn/EN/article/getTxtFile.do?fileType=Ris&id=147","title_en":"Effect of GATA5 inhibiting EpCAM expression on renal carcinoma HEK-293 cells","hasPdf":"true"},"authorNotes_cn":["刘诚，副教授，硕士，主要从事临床医学肿瘤预防与机制研究，E-mail：94749302@qq.com。","关建民，硕士研究生导师，副教授，主要从事肿瘤疾病的基础和临床研究，E-mail：guanjm@126.com。
Effect of GATA5 inhibiting EpCAM expression on renal carcinoma HEK-293 cells
Cheng Liu, Song Shi, Wen Chen, Jianmin Guan
Journal of ShanDong First Medical University&ShanDong Academy of Medical Sciences››2021, Vol. 42››Issue (8): 579-583.
PDF(3148 KB)
PDF(3148 KB)
Effect of GATA5 inhibiting EpCAM expression on renal carcinoma HEK-293 cells
To study the effect of GATA5 on the proliferation regulation of renal cell carcinoma HEK-293 cell line and the expression of cell reprogramming factor EpCAM.
Construction of pTT5-GATA5 expression vector and sequence verification of gene sequence； MTT method to detect cell proliferation rate； cell scratch test to detect cell scratch repair； RT-PCR to detect gene mRNA expression； western blot detect protein expression.
GATA5 inhibits HEK-293 cell proliferation； GATA5 inhibits HEK-293 cell scratch repair； GATA5 inhibits EpCAM mRNA and protein expression.
GATA5 can inhibit the proliferation rate of renal cancer cell HEK-293 cells and inhibit the expression of EpCAM.
| 1 | Liu B，Liu NN，Liu WH，et al. Inhibition of lectin-like oxidized low-density lipoprotein receptor-1 reduces cardiac fibroblast proliferation by suppressing GATA Binding Protein 4［J］. Biochemical and Biophysical Research Communications，2016：S0006291X16308038. |
| 2 | Guo J，Wang ZY，Wu JC， et al. Endothelial SIRT6 Is Vital to Prevent Hypertension and Associated Cardiorenal Injury Through Targeting Nkx3.2-GATA5 Signaling.［J］ .Circ Res， 2019，124： 1448. |
| 3 | Shu J，Zhang K，Zhang M，et al. GATA family members as inducers for cellular reprogramming to pluripotency［J］. Cell Research，2015，25（2）：169. |
| 4 | Song ZP，Chen L，Pang SC， et al. Molecular genetic study on GATA5 gene promoter in acute myocardial infarction.［J］ .PLoS One，2021，16： e0248203. |
| 5 | Akiyama Y，Watkins N，Suzuki H，et al. GATA-4 and GATA-5 transcription factor genes and potential downstream antitumor target genes are epigenetically silenced in colorectal and gastric cancer［J］. Molecular & Cellular Biology，2003，23（23）：8429. |
| 6 | Xia L，Gong Y，Zhang A，et al. Loss of GATA5 expression due to gene promoter methylation induces growth and colony formation of hepatocellular carcinoma cells［J］. Oncology Letters， 2015. |
| 7 | Liu X，Feng Q，Zhang YR，et al. Absence of EpCAM in cervical cancer cells is involved in sluginduced epithelial-mesenchymal transition.［J］ .Cancer Cell Int，2021，21：163. |
| 8 | Song MH，Kim YR，Bae JH，et al. A cancer/testis antigen，NY-SAR-35，induces EpCAM， CD44，and CD133，and activates ERK in HEK293 cells［J］. Biochemical & Biophysical Research Communications，2017，484（2）：298. |
| 9 | Gao Y， Fan WH，Song ZB，et al. Comparison of circulating tumor cell （CTC） detection rates with epithelial cell adhesion molecule （EpCAM） and cell surface vimentin （CSV） antibodies in different solid tumors： a retrospective study［J］ .PeerJ，2021，9： e10777. |
| 10 | Canis M， Lechner A， Mack B， et al. CD133 induces tumour-initiating properties in HEK293 cells［J］. Tumour Biology the Journal of the International Society for Oncodevelopmental Biology & Medicine，2013，34（1）：437. |
| 11 | Shi M，Xie DC，Gaod Y， et al.Targeting miRNAs for pancreatic cancer therapy.［J］ .Curr Pharm Des，2014，20：5279. |
| 12 | Hellebrekers D M E I， Lentjes M H F M，Van d B S M，et al. GATA4 and GATA5 are Potential Tumor Suppressors and Biomarkers in Colorectal Cancer［J］. Clinical Cancer Research，2009， 15（12）：3990. |
| 13 | Amanda O，Aloysius W，Luke E，et al. A Guide to Transient Expression of Membrane Proteins in HEK-293 Cells for Functional Characterization：［J］. Frontiers in Physiology，2016，7：300. |
| 14 | Hu Y，Wu Q，Gao JL，et al. A meta-analysis and The Cancer Genome Atlas data of prostate cancer risk and prognosis using epithelial cell adhesion molecule （EpCAM） expression.［J］ .BMC Urol，2019，19： 67. |
| 15 | Nubel T，Preobraschenski J，Tuncay H，et al. Claudin-7 Regulates EpCAM-Mediated Functions in Tumor Progression［J］. Molecular Cancer Research，2009，7（3）：285. |
| 16 | Lindemans CA，Calafiore M，Mertelsmann AM，et al. Interleukin-22 promotes intestinal-stem-cell-mediated epithelial regeneration.［J］. Nature，2015，528（7583）：560. |
| 17 | Guo M，Akiyama Y，House MG，et al. Hypermethylation of the GATA Genes in Lung Cancer［J］. Clinical Cancer Research An Official Journal of the American Association for Cancer Research，2004，10（23）：7917. |
| 18 | Haworth K E，Kotecha S，Mohun T J，et al. GATA4 and GATA5 are essential for heart and liver development in Xenopus embryos［J］. Bmc Developmental Biology，2008，8（1）：1. |
| 19 | Hellebrekers D M，Lentjes M H，Sm V D B，et al. GATA4 and GATA5 are potential tumor suppressors and biomarkers in colorectal cancer［J］. Clinical Cancer Research，2009，15（12）：3990. |
| 20 | Peters I，Gebauer K，Dubrowinskaja N，et al. GATA5 CpG island hypermethylation is an independent predictor for poor clinical outcome in renal cell carcinoma［J］. Oncology Reports， 2014，31（4）：1523. |
| 21 | Kuan II，Lee CC，Chen CH，et al. The extracellular domain of epithelial cell adhesion molecule （EpCAM） enhances multipotency of mesenchymal stem cells through EGFR-LIN28-LET7 signaling.［J］ .J. Biol. Chem.，2019，294： 7769. |
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