TRPV4-P38α通路在背根神经节持续受压致神经病理性疼痛中的作用

doi:10.3969/j.issn.1004-7115.2021.04.004

betway必威登陆网址（betway.com ）学报 ›› 2021, Vol. 42 ›› Issue (4): 254-257.DOI: 10.3969/j.issn.1004-7115.2021.04.004

TRPV4-P38α通路在背根神经节持续受压致神经病理性疼痛中的作用

张晓¹, 闫平平¹, 张丽娟², 阴涛¹, 高强¹, 郑遵成¹

1.泰安市中心医院康复科,山东泰安 271000;
2.betway必威登陆网址第二附属医院呼吸与危重症医学科,山东泰安 271000

收稿日期:2020-12-10 出版日期:2021-04-25 发布日期:2021-09-01
通讯作者: 郑遵成(1965—),男,山东汶上人,主任医师,硕士,主要从事脊髓损伤及神经修复机制研究。E-mail:zhengzc1965@126.com。
作者简介:张晓(1989—),男,山东泰安人,住院医师,硕士,主要从事神经病理性疼痛的分子机制研究。E-mail:1045103293@qq.com。
基金资助:
山东省医药卫生科技发展计划(2017WS429)。

Role of TRPV4-P38α pathway in dorsal root ganglion induced neuropathic pain

ZHANG Xiao¹, YAN Ping-ping¹, ZHANG Li-juan², YIN Tao¹, GAO Qiang¹, ZHENG Zun-cheng¹

1. Dept. of Rehabilitation, Taian City Center Hospital,Taian 271000,China;
2. Dept. of aspiration and Critical Care Medicine, Second Affiliated Hospital of Shandong First Medical University,Taian 271000,China

Received:2020-12-10 Online:2021-04-25 Published:2021-09-01

摘要/Abstract

摘要： 目的探讨瞬时感受器电位离子通道蛋白4(transient receptor potential vanilloid 4,TRPV4)和P38α在大鼠背根神经节持续受压(chronic compression of dorsal root ganglion,CCD)致神经病理性疼痛中的作用。方法采用健康成年雄性Wistar大鼠,随机分为空白组、CCD组及CCD+钌红组。空白组及CCD手术组于术前1天、术后1天、7天和21天测量机械刺激痛阈(paw withdrawal mechaical threshold,PWMT),CCD+钌红组于术后第7天鞘注钌红前后测量PWMT;采用RT-PCR技术检测各组大鼠术侧背根神经节(dorsal root ganglion,DRG)内TRPV4和P38α基因表达变化。结果与空白组相比,CCD组大鼠术侧PWMT明显下降(P＜0.001),CCD7 d组下降最明显(P＜0.001);同侧DRG内TRPV4和P38α基因表达均明显升高(P＜0.001),且CCD7 d组升高最明显(P＜0.001);鞘注钌红2 h后,CCD组大鼠术侧PWMT明显升高(P＜0.001),同侧DRG内P38α基因表达明显降低(P＜0.001)。结论大鼠CCD术后术侧DRG内TRPV4和P38α基因表达变化与PWMT变化相一致,TRPV4-P38α信号通路可能参与CCD所致神经病理性疼痛的病理过程。

关键词: 背根神经节, 瞬时感受器电位离子通道4, P38α神经病理性疼痛

Abstract: Objective: To investigate the role of transient receptor potential vanilloid 4 (TRPV4) and P38α in neuropathic pain induced by chronic compression of dorsal root ganglion (CCD) in rats.Methods: Healthy adult male Wistar rats were randomly divided into blank group, CCD group and CCD+ruthenium red group. Paw withdrawal mechaical threshold (PWMT) was measured 1 day before surgery, 1 day, 7 days, and 21 days after surgery in the blank group and CCD surgery group. The CCD+ruthenium red group were injected with ruthenium red on the 7th day after surgery, and the PWMT was measured before and after that; RT-PCR was used to detect the expressions of TRPV4 and P38α genes in the dorsal root ganglion (DRG) in each group of the rats.Results: Compared with the blank group, the PWMT of the CCD group decreased significantly on the operation side (P<0.001), and the CCD 7-day group had the most significant decrease (P<0.001). The expressions of TRPV4 and P38α genes in the ipsilateral DRG were significantly increased (P<0.001), and the CCD 7-day group had the most significant increase (P<0.001). After 2 hours of ruthenium red injection, the PWMT of the CCD group significantly increased (P<0.001), while the P38α gene expression in the ipsilateral DRG was significantly reduced (P<0.001).Conclusion: The expressions of TRPV4 and P38α genes in the DRG of the operative rats after CCD are consistent with the changes of PWMT. The TRPV4-P38α signal pathway may be involved in the pathological process of neuropathic pain caused by CCD.

Key words: dorsal root ganglia, transient receptor potential ion channel 4, P38α neuropathic pain

中图分类号:

R741.02

张晓, 闫平平, 张丽娟, 阴涛, 高强, 郑遵成. TRPV4-P38α通路在背根神经节持续受压致神经病理性疼痛中的作用[J]. betway必威登陆网址（betway.com ）学报, 2021, 42(4): 254-257.

ZHANG Xiao, YAN Ping-ping, ZHANG Li-juan, YIN Tao, GAO Qiang, ZHENG Zun-cheng. Role of TRPV4-P38α pathway in dorsal root ganglion induced neuropathic pain[J]. Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences, 2021, 42(4): 254-257.

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TRPV4-P38α通路在背根神经节持续受压致神经病理性疼痛中的作用

Role of TRPV4-P38α pathway in dorsal root ganglion induced neuropathic pain

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