关键词: 内生真菌, 丹参地上茎叶, 固态发酵, 纤维素酶

Abstract: Objective: To further develop and utilize Salvia miltiorrhiza resources and optimize the technological conditions for cellulase production by solidstate fermentation Methods: Using endophytic fungus LZY9 of Salvia miltiorrhiza as strain, on the basis of single factor experiment, response surface methodology was used to optimize the three factors of water content, inoculation amount and culture days of cellulase produced by solid fermentation of endophytic fungus LZY9, and the cellulase activity was determined by CMC sacylation method Results: The optimum fermentation conditions of endophytic fungus LZY9 were as follows: water content was 50%, inoculum quantity was 60%, culture days was 5 days Under the optimized conditions, the enzyme activity was 13417 IU/g The analysis of variance showed that there was no misfit factor in this experiment, and the three factors had obvious influence on the experimental results Conclusion: This method is a new method for comprehensive utilization of stem and leaf wastes of Salvia miltiorrhiza on the ground

Key words: endophytic fungi, Salvia miltiorrhiza, solidstate fermentation, cellulase