家族聚集性与散发性LAA脑梗死患者CYP2C19及GPⅡb/Ⅲa基因表达差异研究

doi:10.3969/j.issn.2097-0005.2021.05.011

betway必威登陆网址（betway.com ）学报 ›› 2021, Vol. 42 ›› Issue (5): 385-389.DOI: 10.3969/j.issn.2097-0005.2021.05.011

家族聚集性与散发性LAA脑梗死患者CYP2C19及GPⅡb/Ⅲa基因表达差异研究

郝瑞¹, 许双双¹, 高敏², 王燕华², 吕玉芹², 王娜², 张敬军²

1.山东省泰山医院,山东泰安 271000;
2.betway必威登陆网址第二附属医院,山东泰安 271000

收稿日期:2020-12-19 出版日期:2021-05-25 发布日期:2021-08-31
通讯作者: 张敬军,主任医师,二级教授,博士,主要从事癫痫及脑血管疾病诊疗及研究工作,E-mail:jjzhang63@126.com。
作者简介:郝瑞,硕士,主要从事老年医学相关疾病研究工作。
基金资助:
山东省高等学校科技计划(J15LL07);betway必威登陆网址学术提升计划(2019QL013);泰安市科技局计划(2017NS0248)。

Study on the difference in expression of CYP2C19 and GPⅡb/Ⅲa between patients with familial LAA atherosclerotic cerebral infarction and those with sporadic LAA cerebral infarction

Hao Rui¹, Xu Shuangshuang¹, Gao Min², Wang Yanhua², Lv Yuqin², Wang Na², Zhang Jingjun²

1. Taishan Hospital of Shandong Province, Taian 271000, China;
2. The Second Affiliated Hospital of Shandong First Medical University, Taian 271000, China

Received:2020-12-19 Online:2021-05-25 Published:2021-08-31

摘要/Abstract

摘要： 目的探讨家族聚集性大动脉粥样硬化性(large-artery atherosclerosis, LAA) 脑梗死患者与散发性LAA脑梗死患者ADP、CYP2C19、P2Y12受体及GPⅡb/Ⅲa等表达差异。方法收集家族聚集性LAA脑梗死和散发性LAA脑梗死患者各25例分别为家族组及散发组,选择25例健康人群作为对照组。分别检测ADP蛋白表达量、CYP2C19基因、P2Y12基因G52T位点及GPⅡb/Ⅲa蛋白表达量。结果家族组与对照组比较ADP表达量差异具有统计学意义(P＜0.05),家族组与散发组及散发组与对照组比较ADP表达量差异无统计学意义(P＞0.05);家族组与对照组比较CYP2C19基因表达明显下调,差异具有统计学意义(P＜0.05);散发组与对照组比较CYP2C19基因表达差异无统计学意义(P＞0.05);P2Y12 基因的G52T位点在家族组、散发组和对照组之间差异有统计学意义 (P＜0.05);家族组与对照组比较GPⅡb/Ⅲa表达量明显上调,差异有统计学意义,家族组与散发组及散发组与对照组比较GPⅡb/Ⅲa表达量差异无统计学意义(P＞0.05);家族组及散发组治疗3个月后mRS评分和神经功能缺损恢复情况比较差异均有统计学意义(P＜0.05)。结论家族组患者与散发组患者ADP蛋白表达、CYP2C19基因、P2Y12基因G52T位点及GPⅡb/Ⅲa蛋白表达等存在明显差异。

关键词: 脑血管病, 大动脉粥样硬化性脑梗死, 基因, CYP2C19

Abstract: Objective: To investigate the differences in expression of ADP, CYP2C19, P2Y12 receptors and GPⅡb/Ⅲa between patients with familial atherosclerotic (large-artery atherosclerosis, LAA) cerebral infarction and those with sporadic LAA cerebral infarction. Methods: Twenty-five patients with familial aggregated LAA cerebral infarction and sporadic LAA cerebral infarction were collected as family group and sporadic group respectively .Twenty-five healthy people were selected as control group. ADP protein expression, CYP2C19 gene, P2Y12 gene G52T locus and GPⅡb/Ⅲa protein expression were detected respectively. Results: There was significant difference in ADP expression between the family group and the control group; There was no significant difference between the family group and the sporadic group and between the sporadic group and the control group; Compared with the control group, the expression of CYP2C19 genes in the family group was significantly down-regulated, and the difference was statistically significant; Compared with the control group, the expression of CYP2C19 genes in the sporadic group had no significant difference in gene expression; The G52T loci of P2Y12 gene were significantly different between family group, sporadic group and control group. The expression of GPⅡb/Ⅲa in family group and control group was significantly up-regulated, and the difference was statistically significant. The difference in the expression of GPⅡb/Ⅲa. between family group and sporadic group and between sporadic group and control group was not significant; After 3 months of treatment, mRS scores of the family group and the sporadic group were compared, and the difference between the two groups was statistically significant. Conclusion: There are significant differences in ADP protein expression, CYP2C19 gene, P2Y12 gene G52T locus and GPⅡb/Ⅲa protein expression between family group and sporadic group.

Key words: cerebrovascular diseases, large-artery atherosclerosis, cerebral infarction, gene;CYP2C19

中图分类号:

R739.41

郝瑞, 许双双, 高敏, 王燕华, 吕玉芹, 王娜, 张敬军. 家族聚集性与散发性LAA脑梗死患者CYP2C19及GPⅡb/Ⅲa基因表达差异研究[J]. betway必威登陆网址（betway.com ）学报, 2021, 42(5): 385-389.

Hao Rui, Xu Shuangshuang, Gao Min, Wang Yanhua, Lv Yuqin, Wang Na, Zhang Jingjun. Study on the difference in expression of CYP2C19 and GPⅡb/Ⅲa between patients with familial LAA atherosclerotic cerebral infarction and those with sporadic LAA cerebral infarction[J]. Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences, 2021, 42(5): 385-389.

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家族聚集性与散发性LAA脑梗死患者CYP2C19及GPⅡb/Ⅲa基因表达差异研究

Study on the difference in expression of CYP2C19 and GPⅡb/Ⅲa between patients with familial LAA atherosclerotic cerebral infarction and those with sporadic LAA cerebral infarction

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