Sox2在人前列腺癌中的表达及其临床意义

国际肿瘤学杂志››2014,Vol. 41››Issue (11): 875-878.doi:10.3760/cma.j.issn.1673-422X.2014.11.023

Sox2在人前列腺癌中的表达及其临床意义

河北省唐山市人民医院普外科（张振宇、张宇峰）；河北省唐山市第二医院重症医学科(张宏伟)

出版日期:2014-12-03发布日期:2015-01-20
通讯作者:张宏伟，Email：zhanghongweiicu@sina.com

Expression and clinical significance of transcription factor Sox2 in human prostate cancer

Department of General Surgery, Tangshan People′s Hospital, Tangshan 063000, China

Online:2014-12-03Published:2015-01-20
Contact:Zhang Hongwei, Email: zhanghongweiicu@sina.com

摘要/Abstract

摘要：目的检测Sox2在人前列腺癌和癌旁组织中的表达情况，并分析其与前列腺癌病理分级和临床分期的关系。方法分别采用免疫组织化学、Western杂交及反转录聚合酶链反应（RTPCR）检测86例前列腺癌组织标本和40例癌旁组织标本中Sox2的表达情况。分析其与前列腺癌病理分级和临床分期之间的相关性。结果免疫组织化学检测结果显示前列腺癌组织中Sox2表达显著高于癌旁组织，阳性表达率分别为79.1%和7.5%，差异有统计学意义(χ2=53.98，P<0.05)；在前列腺癌组织中Sox2的表达与Gleason评分之间相关性具有统计学意义(χ2=19.49，P<0.05)；在淋巴结转移组和非淋巴结转移组Sox2的阳性表达率分别为92.3%和73.3%，差异有统计学意义(χ2=3.95，P<0.05)；但在不同临床分期的前列腺癌组织中Sox2的表达差异无统计学意义（χ2=0.16，P>0.05）。Western杂交及RTPCR检测结果显示前列腺癌组织中Sox2表达显著高于癌旁组织，在淋巴结转移组和非淋巴结转移组之间Sox2表达差异具有统计学意义(t=-2.93，P<0.05； t=3.29，P<0.05)。结论Sox2的表达差异与前列腺癌的Gleason评分和淋巴结转移密切相关,而与临床分期无关，Sox2可能在前列腺癌的发生、分化和转移过程中起重要作用。

关键词:前列腺肿瘤；基因表达； Sox2

Abstract:ObjectiveTo study the expression of Sox2 in human prostate cancer and pericarcinomatous tissue, and the relation between its expression and the pathological type and clinical stage of prostate cancer. MethodsImmunohistochemisty, Western blot and reverse transcriptionpolymerase chain reaction (RTPCR) were used to detect the expression of Sox2 in 86 cases of prostate cancer tissues and 40 cases of pericarcinomatous tissues. The relation between Sox2 expression and the pathological type and clinical stage of prostate cancer was analyzed. ResultsThe expression of Sox2 in prostate cancer tissue (71.9%) was significantly higher than that in pericarcinomatous tissue (7.5%), and the difference was statistically significant (χ2=53.98, P<0.05). Sox2 expression in prostate cancer tissues was significantly related to Gleason score (χ2=19.49, P<0.05). The positive rate of Sox2 in lymphatic metastasis group and negative lymphatic metastasis group was 92.3％ and 73.3％ respectively, and the difference was statistically significant (χ2=3.95, P<0.05)．While there was no significant difference among clinical stages (χ2=0.16, P>0.05). Moreover, Western blot and RTPCR revealed that the expression of Sox2 in prostate cancer tissue was significantly higher than that in pericarcinomatous tissue (t=-2.93, P<0.05; t=3.29, P<0.05). ConclusionThe high expression of Sox2 is closely related to the Gleason score and lymphatic metastasis of prostate cancer, but not to the clinical stage. The overexpression of Sox2 may play an important role in formation, differentiation and metastasis of prostate cancer.

Key words:Prostatic neoplasms,Gene expression,Sox2

张振宇, 张宇锋, 张宏伟. Sox2在人前列腺癌中的表达及其临床意义[J]. 国际肿瘤学杂志, 2014, 41(11): 875-878.

ZHANG Zhen-Yu, ZHANG Yu-Feng, ZHANG Hong-Wei. Expression and clinical significance of transcription factor Sox2 in human prostate cancer[J]. Journal of International Oncology, 2014, 41(11): 875-878.

参考文献

［1］ Ferlay J, Shin HR, Bray F, et al. Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008［J］. Int J Cancer, 2010, 127(12):28932917. ［2］叶定伟, 李长岭. 前列腺癌发病趋势的回顾和展望［J］. 中国癌症杂志, 2007, 17(3):177180. ［3］ Ferrari S, Harley VR, Pontiggia A, et al. SRY, like HMG1, recognizes sharp angles in DNA［J］. EMBO J, 1992, 11(12):44974450. ［4］ Weiss MA. Floppy SOX: mutual induced fit in hmg (highmobility group) boxDNA recognition［J］. Mol Endocrinol, 2001, 15(3): 353362. ［5］ Lin F, Lin P, Zhao D, et al. Sox2 targets cyclinE, p27 and survivin to regulate androgenindependent human prostate cancer cell proliferation and apoptosis［J］. Cell Prolif, 2012, 45(3):207216. ［6］ Jia X, Li X, Xu Y, et al. SOX2 promotes tumorigenesis and increases the antiapoptotic property of human prostate cancer cell［J］. J Mol Cell Biol, 2011, 3(4):230238. ［7］晏佳, 王亚旭. 肿瘤干细胞研究进展［J］. 国际肿瘤学杂志, 2010, 37(11):815817. ［8］ Wicha MS, Liu S, Dontu G. Cancer stem cells: an old ideaa paradigm shift［J］. Cancer Res, 2006, 66(4):18831890. ［9］ Li YQ. Master stem cell transcription factors and signaling regulation［J］. Cell Reprogram, 2010, 12(1):313. ［10］ Adachi K, Suemori H, Yasuda SY, et al. Role of SOX2 in maintaining pluripotency of human embryonic stem cells［J］. Genes Cells, 2010, 15(5):455470. ［11］ Tian T, Zhang Y, Wang S, et al. Sox2 enhances the tumorigenicity and chemoresistance of cancer stemlike cells derived from gastric cancer［J］. J Biomed Res, 2012, 26(5):336345. ［12］ Brcic L, Sherer CK, Shuai Y, et al. Morphologic and clinicopathologic features of lung squamous cell carcinomas expressing Sox2［J］. Am J Clin Pathol, 2012, 138(5):712718. ［13］ Lengerke C, Fehm T, KuilhR, et al. Expression of the embryonic stemcellmarker SOX2 in earlystage breast carcinoma［J］. BMC Cancer, 2011, 11:42. ［14］ HerrerosVillanueva M, Zhang JS, Koenig A, et al. SOX2 promotes dedifferentiation and imparts stem celllike features to pancreatic cancer cells［J］. Oncogenesis, 2013, 2:e61. ［15］ Han X, Fang X, Lou X, et al. Silencing SOX2 induced mesenchymalepithelial transition and its expression predicts liver and lymph node metastasis of CRC patients［J］. PLoS One, 2012,7(8):e41335. ［16］ Bader P, Burkard FC, Markwalder R, et al. Disease progression andsurvival of patients with positive lymph nodes after radical prostatectomy．Is there a chance for cure?［J］. J Urol, 2003, 169(3):849854.