Effects of decorin on proliferation, migration and invasion of bladder cancer cells

Abstract

Abstract:

ObjectiveTo investigate the effects of decorin (DCN) on the proliferation, migration and invasion of bladder cancer cells.MethodsBladder cancer T24 cell line was used as the research object. MTT assay was used to detect the inhibitory effect of DCN at different concentrations (0, 5, 10, 20, 30, 40, 50 mg/L) on T24 cell proliferation at 24, 48, 72 and 96 h. The effects of DCN on T24 cell cycle and apoptosis were analyzed by flow cytometry. MTT assay, Transwell migration and invasion experiments were used to detect the effects of DCN on the adhesion, migration and invasion ability of T24 cells. The effects of DCN on TGF-β1 and P21 protein expression were detected by ELISA and Western blotting.ResultsT24 cells were treated with 0, 5, 10, 20, 30, 40 and 50 mg/L DCN at 24, 48, 72 and 96 h, and there were statistically significant diffe-rences in cell proliferation activity (F=168.64,P<0.001;F=165.81,P<0.001;F=291.02,P<0.001;F=148.93,P<0.001). T24 cells were treated with 0, 5, 10, 20, 30, 40 and 50 mg/L DCN for 72 h, and the cell proliferation activities were (60.71±3.03)%, (40.82±2.09)%, (37.24±1.63)%, (25.65±2.55)%, (23.00±2.67)%, (10.78±1.17)%, (11.04±0.96)%, respectively, and there was a statistically significant difference. At the concentration of 40 mg/L, the proliferation activity reached the lowest level, and the inhibitory effect on cell proliferation was the strongest. At concentrations of 40 and 50 mg/L, the cells in G₁phase reached the peak value, while the cells in S phase reached the lowest value, and the cells in G₂phase remained unchanged throughout the treatment process. T24 cells were treated with 0, 5, 10, 20, 30, 40 and 50 mg/L DCN for 72 h, and the apoptosis rates of cells were (12.18±1.17)%, (21.24±1.05)%, (19.80±1.20)%, (26.52±1.40)%, (30.86±1.40)%, (52.99±1.22)%, (43.04±2.16)%, respectively, and there was a statistically significant difference (F=178.54,P<0.001). The differences between 5, 10, 20, 30, 40, 50 mg/L DCN and 0 mg/L DCN were all statistically significant (allP<0.001). When T24 cells were treated with 0, 40 mg/L DCN for 72 h, the cell adhesion rates were (37.14±1.35)% and (59.86±1.95)%, the numbers of migrated cells were 53.86±3.18 and 12.86±1.35, and there were statistically significant differences (t=25.25,P<0.001;t=31.36,P<0.001). When DCN was applied to T24 cells for 48 h, the numbers of invasion at 0, 40 mg/L were 235.14±3.44 and 160.86±3.13, and there was a statistically significant difference (t=2.27,P<0.001). When T24 cells were treated with 0, 5, 10, 20, 30, 40 and 50 mg/L DCN for 72 h, the relative expression levels of TGF-β1 were 85.67±3.35, 45.51±1.19, 49.93±4.15, 47.64±3.53, 46.05±3.18, 25.54±2.25, 33.44±4.05, and there was a statistically significant difference (F=324.58,P<0.001). Compared with 0 mg/L DCN, 5, 10, 20, 30, 40 and 50 mg/L DCN could significantly inhibited the expression of TGF-β1 (allP<0.001). Compared with 0 mg/L DCN, P21 protein was upregulated 72 h after treatment with 40 mg/L DCN.ConclusionDCN can inhibit proliferation and induce apoptosis of T24 cellsin vitro, and has the effect of anti-metastasis of T24 cells.

Key words:Urinary bladder neoplasms,Cell movement,Neoplasm invasiveness,Decorin

Wang Ziyi, Chen Hongjie, Yang Ninggang, Zhang Jun, Zhang Xiangjun, Yu Xinning, Ma Zhongyi, Dai Enlai. Effects of decorin on proliferation, migration and invasion of bladder cancer cells[J]. Journal of International Oncology, 2021, 48(6): 335-340.

Figures/Tables7

DCN浓度(mg/L)	24 h	48 h	72 h	96 h
0	59.70±2.10	60.47±1.81	60.71±3.03^a	59.47±1.81
5	39.00±1.43	52.73±2.27	40.82±2.09^a	52.20±2.20
10	36.09±1.93	44.99±3.00	37.24±1.63^a	45.49±2.38
20	28.03±1.91	39.83±2.11	25.65±2.55^a	40.71±2.92
30	24.18±1.99	31.41±2.46	23.00±2.67^a	32.81±2.24
40	22.35±1.59	26.28±1.84	10.78±1.17	27.49±1.28
50	22.19±0.93	26.46±2.31	11.04±0.96	27.01±1.84
F值	168.64	165.81	291.02	148.93
P值	<0.001	<0.001	<0.001	<0.001

DCN浓度(mg/L)	细胞周期停滞比率	细胞凋亡率
0	38.86±0.63^ab	60.87±0.67^ab	1.76±0.59	12.18±1.17^ab
5	37.12±0.75^ab	60.83±0.84^ab	2.06±1.59	21.24±1.05^abc
10	36.61±0.40^ab	61.73±2.09^ab	1.67±1.69	19.80±1.20^abc
20	36.23±1.39^ab	60.34±1.39^ab	2.95±0.69	26.52±1.40^abc
30	38.73±2.10^ab	59.13±1.43^ab	2.15±0.67	30.86±1.40^abc
40	69.79±0.62	26.74±0.67	3.48±1.29	52.99±1.22^b
50	71.05±1.18	27.19±1.67	1.77±0.14	43.04±2.16
F值	240.17	238.66	1.66	178.54
P值	<0.001	<0.001	0.530	<0.001

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