Expression levels and clinical significance of serum miR-497 and miR-383 in patients with esophageal cancer

Abstract

Abstract:

ObjectiveTo observe the serum levels of miR-497 and miR-383 in patients with esophageal cancer， and to analyze the clinical significance for esophageal cancer.MethodsEsophageal cancer patients admitted to Union Wuhan Red Cross Hospital from July 2018 to February 2020 were collected as the esophageal cancer group （n=96）， which were divided into a recurrence group （n=29） and a non recurrence group （n=67） based on follow-up results. The control group included healthy individuals who underwent physical examinations at this hospital during the corresponding period （n=83）， and the benign lesion group included patients with benign esophageal lesions who underwent treatment at same hospital during the corresponding period （n=78）. Real time fluorogenic quantitative PCR was applied to detect serum levels of miR-497 and miR-383 in each group； Pearson method was applied to analyze the correlation between serum levels of miR-497 and miR-383 in esophageal cancer patients； Relationship between serum levels of miR-497， miR-383 and clinical pathological characteristics in esophageal cancer patients was analyzed； Receiver operator characteristic （ROC） curve was plotted to analyze the efficacy of serum miR-497， miR-383， and their combination in predicting the prognosis of esophageal cancer patients.ResultsThe serum miR-497 levels in the control group， benign lesion group and the esophageal cancer group were 1.01±0.18， 0.86±0.15， 0.77±0.14， respectively， and the serum miR-383 levels were 1.02±0.21， 0.95±0.15， 0.84±0.15， respectively， with statistically significant differences （F=52.59，P<0.001；F=25.12，P<0.001）； There were statistically significant differences in serum miR-497 and miR-383 levels between any two of the three groups （allP<0.05）. Pearson correlation analysis reveled that serum miR-497 level was positively correlated with miR-383 level in esophageal cancer patients （r=0.46，P<0.001）. There were statistically significant differences in serum miR-497 and miR-383 levels among esophageal cancer patients with different tumor diameters （t=6.58，P<0.001；t=5.06，P<0.001）， lymph node metastases （t=5.55，P<0.001；t=4.63，P<0.001） and TNM stages （t=5.00，P<0.001；t=2.75，P<0.001）. The serum miR-497 （0.83±0.15vs.0.62±0.11，t=6.78，P<0.001） and miR-383 （0.91±0.16vs.0.67±0.13，t=7.12，P<0.001） levels in the non recurrence group were both higher than those in the recurrence group. ROC curve showed that the critical value of serum miR-497 for predicting the prognostic situation of esophageal cancer was 0.72， with a sensitivity of 68.97%， a specificity of 68.66%， and an area under the curve （AUC） of 0.756； The critical value of serum miR-383 was 0.84， with a sensitivity of 82.76%， a specificity of 67.16%， and an AUC of 0.827； The sensitivity of the combination of the two was 79.31%， the specificity was 85.07%， and the AUC was 0.899； The combination of the two had a better prediction effect than that of serum miR-497 （Z=3.31，P=0.001） and miR-383 （Z=2.51，P=0.012） alone.ConclusionThe serum levels of miR-497 and miR-383 in patients with esophageal cancer are lower than those in healthy individuals and patients with benign lesions， which are related to tumor diameter， lymph node metastasis， TNM stage， and prognosis. The combined detection of the two has certain predictive value for the prognosis of esophageal cancer.

Key words:Esophageal neoplasms,Prognosis,miR-497,miR-383

Wan Fang, Yang Gang, Li Rui, Wan Qijing. Expression levels and clinical significance of serum miR-497 and miR-383 in patients with esophageal cancer[J]. Journal of International Oncology, 2024, 51(4): 204-209.

Figures/Tables7

基因	正向引物	反向引物
miR-497	5’-CATACCTGACTGTGTTGCTAAA-3’	5’-CTTTTGAGTGTTGGTGATTTGG-3’
miR-383	5’-TCAGAAGGTGATTGTGGC-3’	5’-GAACATGTCTGCGTATCTC-3’
U6	5’-CTCGCTTCGGCAGCACA-3’	5’-AACGCTTCACGAATTTGCGT-3’

一般资料	对照组（n=83）	良性病变组（n=78）	食管癌组（n=96）	χ²/t值	P值
性别
男	49（59.04）	46（58.97）	58（60.42）	0.05	0.975
女	34（40.96）	32（41.03）	38（39.58）
年龄（岁）	59.36±6.42	58.39±6.71	59.62±6.37	0.83	0.438
体质量指数（kg/m²）	22.37±3.13	23.05±3.99	22.96±3.52	0.90	0.409
收缩压（mmHg）	123.51±14.22	122.63±13.62	123.28±13.86	0.09	0.917
舒张压（mmHg）	76.85±8.72	77.61±9.16	76.19±8.31	0.57	0.565

组别	miR-497	miR-383
对照组（n=83）	1.01±0.18	1.02±0.21
良性病变组（n=78）	0.86±0.15^a	0.95±0.15^a
食管癌组（n=96）	0.77±0.14^ab	0.84±0.15^ab
F值	52.59	25.12
P值	<0.001	<0.001

临床病理特征	例数	miR-497	t值	P值	miR-383	t值	P值
肿瘤直径（cm）
≥4	51	0.68±0.11	6.58	<0.001	0.76±0.14	5.06	<0.001
<4	45	0.87±0.17	6.58	<0.001	0.92±0.17	5.06	<0.001
病理类型
腺癌	34	0.74±0.13	1.37	0.173	0.81±0.14	1.22	0.224
鳞状细胞癌	62	0.78±0.14	1.37	0.173	0.85±0.16	1.22	0.224
组织分化程度
低分化	56	0.77±0.15	0.35	0.728	0.85±0.15	0.97	0.336
中高分化	40	0.76±0.12	0.35	0.728	0.82±0.15	0.97	0.336
淋巴结转移
有	23	0.63±0.12	5.55	<0.001	0.71±0.13	4.63	<0.001
无	73	0.81±0.14	5.55	<0.001	0.88±0.16	4.63	<0.001
TNM分期
Ⅰ~Ⅱ期	59	0.83±0.16	5.00	<0.001	0.87±0.17	2.75	<0.001
Ⅲ~Ⅳ期	37	0.68±0.11	5.00	<0.001	0.78±0.13	2.75	<0.001

组别	miR-497	miR-383
复发组（n=29）	0.62±0.11	0.67±0.13
未复发组（n=67）	0.83±0.15	0.91±0.16
t值	6.78	7.12
P值	<0.001	<0.001

变量	Youden指数	截断值	敏感性（%）	特异性（%）	AUC	95%CI	P值
miR-497	0.376	0.72	68.97	68.66	0.756	0.657~0.838	<0.001
miR-383	0.499	0.84	82.76	67.16	0.827	0.736~0.896	<0.001
二者联合	0.644	-	79.31	85.07	0.899	0.821~0.951	<0.001

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