Abstract:ObjectiveTo explore the relationship between the rs18004197 polymorphism in the 3′untranslated region of adenomatous polyposis coli (APC) gene and colorectal cancer susceptibility. MethodsFirstly, we collected the peripheral venous blood of 573 colorectal cancer cases and 588 controls, and then extracted DNA from blood samples, genotyped rs1804197 polymorphism using realtime PCR and assessed its association with the susceptibility of colorectal cancer. ResultsThere were 387 CC (67.5%), 153 AC (26.7%) and 33 AA (5.8%) genotypes in the colorectal cancer cases. In the control group, there were 427 CC (72.6%), 144 AC (24.5%) and 17 AA (2.9%) genotypes. The AA genotype odds ratio (OR=2.14, 95%CI: 1.173.91, P=0.011) and the A allele frequency (P=0.011) were significant difference in case and control groups. Further subgroup analysis showed that the differences of the frequency distribution in the male (P=0.048) and nondrinking (P=0.020) groups were statistically significant. In the male group, the risk of colorectal cancer was increased by 0.41 (OR=1.41, 95%CI: 1.011.98) for individuals bearing the A allele. In the nondrinking group, the risk of colorectal cancer was increased by 0.22 (OR=1.22, 95%CI: 0.911.64) for individuals bearing the A allele, but the result was not statistically significant. ConclusionThe rs18004197 polymorphism in the 3′untranslated region of APC gene is related to the susceptibility of colorectal cancer. The AA genotype may increase the susceptibility of colorectal cancer.

Key words:Colorectal neoplasms,Genes, APC,Polymorphism, single nucleotide