Abstract:Effect of disodium cantharidinate on in vitro lymphocyte immune response in lung cancer patientsXing Zhenyu*, Zhang Huidi, Gao Lei, Chang Wenlong, Tian Shaohui, Zhang Hui. *Corresponding author: 【Abstract】ObjectiveTo investigate the effect of disodium cantharidinateon in vitro lymphocyte immune response in lung cancer patients. MethodsTwenty nonsmall cell lung cancer patients diagnosed by pathological diagnosis were selected. The effects of different concentrations of disodium cantharidinate and vitamin B6 on lymphocyte proliferation and CD4+CD25+ T cells, CD25+FOXP3+ T cells, CD4+ and CD8+ T cells were detected by methyl thiazolyl tetrazolium assay and flow cytometry. ResultsWhen the concentrations of disodium cantharidinate and vitamin B6 was less than 10 μg/ml, with the growth of concentration, it stimulated the proliferation of peripheral blood lymphocytes. When the concentration was more than 10 μg/ml, with the growth of concentration, the absorbance value decreased, and the stimulating effect weakened. When the concentration of disodium cantharidinate and vitamin B6 injection was less than 10 μg/ml, with the growth of concentration, CD4+/CD8+ ratio increased significantly, and CD4+ CD25+/CD4+ and CD25+ FOXP3+ T cells were significantly lower, which showed statistically significant differences （t=2.171, P=0.032; t=2.103, P=0.041; t=3.662, P=0.002; t=3.201, P=0.003; t=3.233, P=0.003). But when the concentration was more than 10 μg/ml, The differences of CD4+/CD8+ ratio, CD4+CD25+/CD4+ and CD25+FOXP3+ T cells in 10, 15, 20 μg/ml groups were not statistically significant, which showed that disodium cantharidinate and vitamin B6 injection had a positive effect on enhancing the immune function of lymphocytes in lung cancer in a concentration dependent manner, but high concentration was unhelpful. ConclusionDisodium cantharidinate and vitamin B6 injection can promote lung cancer in vitro lymphocyte proliferation as well as increase its immunity in a certain range.

Key words:Lung neoplasms,Cell culture techniques,Autoimmunity,Disodium cantharidinate