Study on the mechanism of human large cell lung cancer NCIH661 cells apoptosis induced by crotoxin

Abstract

Abstract:ObjectiveTo observe the apoptosis of human large cell lung cancer NCIH661 cells induced by crotoxin, and to explore its mechanism. MethodsThe growth suppression of crotoxin on the NCIH661 cells was detected by CCK8 colorimetry, and the formation of NCIH661 cells was observed by the plat colony experiment. This experiment included 4 groups: negative control group, crotoxin group (60 μg/ml crotoxin acted for 24 h), crotoxin +SB203580 group (pretreated cells using 5 μmol/L SB203580 for 1 h, then 60 μg/ml crotoxin acted for 24 h), SB203580 group (pretreated cells using 5 μmol/L SB203580 for 1 h, then cultivated cells using complete culture solution). They were detected that the cell cycle and apoptosis rate of NCIH661 cells treated with crotoxin by the flow cytometry. Additionally, they were tested that the change of the cell cycle and apoptosis rate after the NCIH661 cells were treated with crotoxin and the activity of p38MAPK was inhibited by SB203580. ResultsWhen the concentration of crotoxin was greater than or equal to 30 μg/ml, the inhibitory effect of crotoxin on the activity of NCIH661 cells and colony formation, and inhibition rate rose with increasing function of time and drug concentration. Flow cytometry showed that the apoptosis rate of crotoxin group and crotoxin+SB203580 group were (16.70±1.38)% and (2.15±0.54)%, compared to the control group (1.47±0.29)%, and the former difference was statistically significant and the latter was not statistically significant(t=-18.763, P=0.000; t=-1.935, P=0.125). The G1 period cells of crotoxin group and crotoxin+SB203580 group were (57.25±1.09)% and (48.04±1.03)%, compared to the control group (47.46±0.69)%, and the former difference was statistically significant and the latter was not statistically significant(t=-13.124, P=0.000; t=-0.809, P=0.464). ConclusionCrotoxin can promote the apoptosis of human large cell lung cancer NCIH661 cells, and this effect may be related to the excitation of p38MAPK signal pathway.

Key words:Neurotoxins,Carcinoma, nonsmallcell lung,Apoptosis,p38MAPK

Li Rui, He Jingkang, Shen Jian, Shi Zhen, Li Wei, Yu Xiaojun. Study on the mechanism of human large cell lung cancer NCIH661 cells apoptosis induced by crotoxin[J]. Journal of International Oncology, 2015, 42(7): 481-484.

References

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