Abstract:Objective To investigate the serum levels and clinical significances of microRNA-335 (miR-335) and microRNA-155 (miR-155) in patients with primary gallbladder cancer (PCG). Methods A total of 96 PCG patients (PCG group) and 50 healthy controls (control group) admitted to the Second People′s Hospital of Hainan Province from January 2016 to October 2018 were selected. Real-time quantitative PCR (RT-PCR) was used to detect the serum levels of miR-335 and miR-155 in each group. The relationships between miR335 and miR-155 levels and clinical pathological characteristics of PCG patients were analyzed. The diagnostic value of miR-335 and miR-155 in PCG was analyzed by ROC curve. Results The serum level of miR-335 in PCG group was significantly lower than that in the control group (1.50±0.42 vs. 3.65±1.18, t=10.319, P＜0.001). The serum level of miR-155 in PCG group was significantly higher than that in the control group (3.18±0.61 vs. 0.74±0.12, t=13.627, P＜0.001). The serum levels of miR-335 and miR-155 in PCG patients were correlated with TNM stage (t=4.863, P=0.024; t=5.117, P=0.008) and lymph node metastasis (t=5.725, P＜0.001; t=6.802, P＜0.001). ROC curve analysis showed that the critical values of serum miR-335 and miR-155 for diagnosing PCG were 1.18 and 2.35, respectively. The area under the curve of the two combined diagnosis of PCG (0.920, 95%CI: 0.863-0.977) was the largest, with sensitivity and specificity of 93.8% and 85.7%. Conclusion The low serum level of miR335 and high level of miR155 are associated with the higher TNM stage and lymph node metastasis of PCG, and the combined detection of the two is helpful to improve the diagnostic rate of PCG.

Key words:Gallbladder neoplasms,MicroRNAs