Abstract:Objective To investigate the significance of metastasis associated lung adenocarcinoma transcript 1 (MALAT1),cyclooxygenase-2 (COX-2),beta catenin (β-catenin)、matrix metalloproteinase (MMP)-3 and MMP-9 in the occurrence and development of colorectal carcinoma. Methods Real-time PCR was used to detect MALAT1,COX-2,β-catenin,MMP-3 and MMP-9 mRNA expression in samples from 30 fresh colorectal carcinomas and 30 corresponding adjacent tissues. And the correlation analysis of the gender and age of patients, CEA, immune cellular factors (CD4 and CD8), clinical stages, and the degree of differentiation was undertaken. Results The expression levels of MALAT1,COX-2,β-catenin and MMP-9 were significantly different between colorectal carcinoma tissues and adjacent colorectal tissues (P<0.05). MMP-3 showed no significant difference (P>0.05). MALAT1,COX-2,β-catenin and MMP-9 expression levels showed an average 2.22fold, 1.86fold, 2.16fold, 0.58fold (P<0.01) increase in colorectal carcinoma tissues when compared with adjacent colorectal tissues respectively. There were negative correlation between MALAT1 and β-catenin (colorectal carcinoma tissues vs adjacent colorectal tissues) (r=-0.346, P=0.030). While there were positive correlation between MMP-9 and β-catenin (colorectal carcinoma tissues vs adjacent colorectal tissues) (r=0.312, P=0.047). There were significant difference between male patients and female patients in terms of COX-2 and MMP-9 (colorectal carcinoma tissues vs adjacent colorectal tissues) (P= 0.047; P=0.018). There were significant difference between patients with tumor marker CEA increase and patients without CEA increase in terms of COX-2 (colorectal carcinoma tissues vs adjacent colorectal tissues) (P=0.021). Conclusion MALAT1,COX-2,MMP-9 and β-catenin have significance in the occurrence and development of colorectal carcinoma, while MMP-3 has no significant reference value. The negative correlation between MALAT1 and β-catenin and the positive correlation between MMP-9 and β-catenin might show some interaction relationship in the development of colorectal carcinoma. The expression differences of COX-2 and MMP-9 (colorectal carcinoma tissues vs adjacent colorectal tissues) in male and female patients suggest that above two genes may affect the occurrence ratio of colorectal carcinoma. Detecting of COX-2 maybe helpful to the tumor marker CEA during the diagnosis of colorectal carcinoma.

Key words:Colorectal neoplasms,Cyclooxygenase 2,beta Catenin,Matrix metalloproteinase 3,Matrix metalloproteinas 9,Metastasis associated lung adenocarcinoma transcript 1