目的 建立同时分离测定萹蓄药材中绿原酸、槲皮苷、杨梅苷、金丝桃苷、木犀草苷、槲皮素、山奈素、咖啡酸等8种有效成分含量的高效液相色谱方法。方法 采用Ultimate XB-C
18色谱柱(4.6 mm× 250 mm,5 μm),以乙腈-0.5%磷酸水为流动相进行梯度洗脱;柱温45 ℃;流速 1.0 mL/min;检测波长360 nm。再对河北产三批次萹蓄药材进行成分分析。结果 8种成分在1~40 μg/mL浓度范围内与峰面积均呈良好的线性关系,重复性、精密度和稳定性实验都符合要求,加样回收率为97.62%~111.16%。三批次萹蓄药材成分含量及比重有差异。结论 HPLC法简便、准确、快速、重现性好,可用于萹蓄药材中这8种成分含量的同时测定。
Abstract
Objective: To develop an HPLC method for simultaneous determination of chlorogenic acid, caffeic acid, myricitrin, hyperoside, galuteolin, quercitrin, quercetin and galangal in
polygonum aviculareL.. Methods: The analysis was performed on an Ultimate XB-C
18column (4.6 mm×250 mm, 5 μm) with gradient elution. The mobile phases were 0.5% aqueousphosphoric acid and acetonitrile. The detection wave length was 360 nm. The column temperature was set at 45 ℃. And component content of
polygonum aviculareL. in 3 batches from Hebei province was analysed. Results: The eight marker components were separated well with good linearity, precision, stability and repeatability. The recovery rate was 97.62%-111.16%.
polygonum aviculareL. in 3 batches from Hebei province had different component contents and proportions. Conclusion: The described method is sensitive, convenient and accurate and is suitable for the simultaneous determination of chlorogenic acid, caffeic acid, myricitrin, hyperoside, galuteolin, quercitrin, quercetin and galangal in
polygonum aviculareL.
关键词
HPLC/萹蓄/成分含量分析
Key words
HPLC/polygonum aviculareL./component content analysis
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