睡眠剥夺后大鼠下丘脑Orexin-A神经元的表达及其对学习记忆的影响

doi:10.3969/j.issn.2097-0005.2021.05.010

betway必威登陆网址（betway.com ）学报 ›› 2021, Vol. 42 ›› Issue (5): 380-384.DOI: 10.3969/j.issn.2097-0005.2021.05.010

睡眠剥夺后大鼠下丘脑Orexin-A神经元的表达及其对学习记忆的影响

倪丽艳¹, 唐吉友²

1.巨野县人民医院,山东巨野 274900;
2.betway必威登陆网址第一附属医院,山东济南 250014

收稿日期:2021-01-25 出版日期:2021-05-25 发布日期:2021-08-31
作者简介:倪丽艳,硕士研究生,主治医师,研究方向:神经病学,E-mail:niliyan122@163.com。
基金资助:
国家自然科学基金 (81471345)。

The expression of Orexin-A neurons in the hypothalamus of rats after sleep deprivation and its effect on learning and memory

Ni Liyan¹, Tang Jiyou²

1. Juye County People's Hospital,Juye 274900,China;
2. The First Affiliated Hospital of Shandong First Medical University, Jinan 250014,China

Received:2021-01-25 Online:2021-05-25 Published:2021-08-31

摘要/Abstract

摘要： 目的探讨大鼠睡眠剥夺后下丘脑Orexin-A的表达及其对空间学习记忆能力的影响。方法健康成年雄性Wistar大鼠随机分为空白对照组(CC)、环境对照组(TC)、睡眠剥夺组(SD)。采用间歇性跑步机进行72 h全睡眠剥夺。免疫组化观察各组大鼠下丘脑Orexin-A表达,Morris水迷宫试验测试各组大鼠的空间学习记忆能力。结果 SD组大鼠下丘脑Orexin-A阳性细胞数较TC和CC组显著增加,差异有统计学意义(P<0.01),TC组与CC组相比差异无统计学意义(P＞0.05);与TC组相比,SD组逃避潜伏期明显延长,穿越平台象限的次数明显减少,差异有统计学意义(P＜0.01),TC与CC组比较差异无统计学意义(P＞0.05)。结论睡眠剥夺可能通过诱导下丘脑Orexin-A的过度表达损害大鼠的空间学习记忆能力。

关键词: 睡眠剥夺, 学习记忆, Orexin-A

Abstract: Objective: To investigate the expression of Orexin-A neurons in the hypothalamus of rats after sleep deprivation and its effect on learning and memory.Methods: Healthy adult male Wistar rats were randomly divided into cage control (CC), treadmill control (TC), sleep deprivation (SD) group. 72h total sleep deprivation was performed by intermittent treadmill. The expression of Orexin-A was observed by immunohistochemistry, and the spatial learning and memory ability was tested by Morris water maze test. Results: Compared with TC, the number of Orexin-A positive cells in hypothalamus of SD rats was significantly increased (P<0.01). There was no significant difference between TC and CC group (P>0.05). Compared with TC group, the escape latency and the times of crossing platform quadrant in SD group were significantly longer (P<0.01), and there was no significant difference between TC group and CC group (P>0.05). Conclusion: Sleep deprivation may impair spatial learning and memory in rats by inducing overexpression of Orexin-A in hypothalamus.

Key words: sleep deprivation, learning and memory, Orexin-A

中图分类号:

R741.02

倪丽艳, 唐吉友. 睡眠剥夺后大鼠下丘脑Orexin-A神经元的表达及其对学习记忆的影响[J]. betway必威登陆网址（betway.com ）学报, 2021, 42(5): 380-384.

Ni Liyan, Tang Jiyou. The expression of Orexin-A neurons in the hypothalamus of rats after sleep deprivation and its effect on learning and memory[J]. Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences, 2021, 42(5): 380-384.

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睡眠剥夺后大鼠下丘脑Orexin-A神经元的表达及其对学习记忆的影响

The expression of Orexin-A neurons in the hypothalamus of rats after sleep deprivation and its effect on learning and memory

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