Abstract: Objective: To further develop and utilize Salvia miltiorrhiza resources and optimize the technological conditions for cellulase production by solid-state fermentation. Methods: Using endophytic fungus LZY9 of Salvia miltiorrhiza as strain, on the basis of single factor experiment, response surface methodology was used to optimize the three factors of water content, inoculation amount and culture days of cellulase produced by solid fermentation of endophytic fungus LZY9, and the cellulase activity was determined by CMC sacylation method. Results: The optimum fermentation conditions of endophytic fungus LZY9 were as follows: water content was 5.0%, inoculum quantity was 60%, culture days was 5 days. Under the optimized conditions, the enzyme activity was 13.417 IU/g. The analysis of variance showed that there was no misfit factor in this experiment, and the three factors had obvious influence on the experimental results. Conclusion: This method is a new method for comprehensive utilization of stem and leaf wastes of Salvia miltiorrhiza on the ground.

Key words: endophytic fungi, Salvia miltiorrhiza, solid-state fermentation, cellulase

摘要： 目的 进一步开发利用丹参资源,优化固态发酵产纤维素酶的工艺条件。方法 以丹参内生真菌LZY9为菌种,在单因素试验的基础上,利用响应面法对内生真菌LZY9固体发酵产纤维素酶的含水量、接菌量和培养天数3个因素进行优化,通过CMC糖化法测定纤维素酶活力。结果 内生真菌LZY9的最佳发酵工艺条件为:含水量为5.0%,接种量为60%,培养天数为5天,在此优化条件下,酶活力为13.417 IU/g。方差分析表明本试验没有失拟因素,3种因素对试验结果影响明显。结论 本方法为丹参地上茎叶废弃物的综合利用提供了新的方法。

关键词: 内生真菌, 丹参地上茎叶, 固态发酵, 纤维素酶