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Clinical effects of minimally invasive thoracoscopic surgery and traditional surgery on primary mediastinal tumors Bi Siyun Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (6): 448-450.   DOI: 10.3969/j.issn.2097-0005.2021.06.014 Abstract （185）      PDF （2254KB）（65）       Knowledge map    Save Objective: To investigate the clinical effect of traditional surgery and minimally invasive surgery via thoracoscopy (VATS) for primary mediastinal tumor. Methods: A total of 94 patients with primary mediastinal tumor admitted to Juye People's Hospital from February 2018 to June 2019 were selected as the study objects. They were randomly divided into two groups according to the digital randomization method, among which the control group was given traditional surgical treatment, while the observation group was treated with minimally invasive surgery via thoracoscopy to compare the therapeutic effects of the two groups. Results: The duration of hospitalization, drainage, analgesic use and operation was shorter in the observation group than those in the control group ( P＜0.05) and intraoperative blood loss was less than that of the control group ( P＜0.05); Compared with the control group, the incidence of postoperative complications in the observation group was low, and the differences between the groups were significant ( P＜0.05); At the same time, the postoperative serum inflammatory factor levels of the two groups were statistically significant ( P＜0.05). Conclusion: The application of minimally invasive surgery via thoracoscopy in the treatment of patients with primary mediastinal tumor can not only reduce the body's inflammatory response, but also reduce postoperative complications and shorten the length of hospital stay. Reference | Related Articles | Metrics

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Clinical value of minimally invasive ankle arthrodesis in the treatment of traumatic ankle arthritis Xu Dong Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (6): 460-462.   DOI: 10.3969/j.issn.2097-0005.2021.06.018 Abstract （172）      PDF （2783KB）（65）       Knowledge map    Save Objective: To investigate the clinical value of arthroscope-assisted ankle arthrodesis in the treatment of traumatic arthritis of foot and ankle. Methods: From February 2017 to May 2019, retrospective analysis of the 43 cases of the clinical data of patients with severe traumatic ankle arthritis was made, 23 cases of ankle arthrodesis was performed arthroscopically (minimally invasive group), 20 patients who underwent open ankle arthrodesis (open group), and postoperative pain score difference of the day and 7 days, and osseous fusion rate after 6 months and 1 year and the differences between the AOFAS ankle function score were compared two groups of patients. Results: The pain scores of the minimally invasive group were (4.18±1.10) and (1.55±0.52) 1 and 7 days after surgery, which were both lower than those of the open group(5.54±1.15)and(3.45±0.58).The differences were statistically significant ( t=-3.959,-11.327, P<0.001). The bone fusion rate of the minimally invasive group was 82.61% 6 months after surgery, which was significantly higher than that of the open group(35.00%).The difference was statistically significant ( χ 2=10.143, P＜0.001). Bone fusion was formed in both groups at 1 year after surgery. Six months after surgery, there was no difference in AOFAS ankle function score between the two groups ( P>0.05).AOFAS score was (77.55±5.70) in the minimally invasive group and (66.49±4.55) in the open group 1 year after surgery, and the difference between the two groups was statistically significant( t=6.977, P<0.001). Conclusion: Minimally invasive ankle arthrodesis has more advantages in the treatment of severe traumatic arthritis of foot and ankle, which can reduce postoperative pain, improve the rate of early bone fusion and accelerate the recovery of foot and ankle function, which is worthy of further basic and clinical studies. Reference | Related Articles | Metrics

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Inhibitory effect of VEGF and Survivin antisense phosphorothioate oligodeoxynucleotides on the growth of human tongue cancer xenografts in nude mice Li Tengyu, Yu Xiaopeng, Li Xiaoguang, Wang Yanxiu, Li Junfu, Chen Daiyun, Sun Zhen Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (6): 401-405.   DOI: 10.3969/j.issn.2097-0005.2021.06.001 Abstract （189）      PDF （7996KB）（53）       Knowledge map    Save Objective: To study the effect of VEGF ASODN and Survivin ASODN co transfection on Tca8113 tongue cancer cells in nude mice. Methods: 25 female nude mice were divided into 5 groups with 5 mice in each group. ①In the combined ASODN group, 66 μg survivin ASODN and 66 μg VEGF ASODN were injected into the tumor body and peritumoral area of nude mice . The volume of injection was 200 μl. It contains lipofectaminetm 2000 and serum-free 1640 solution. ②VEGF ASODN group:VEGF ASODN 66 μg was injected in the same way . ③Survivin ASODN group:survivin ASODN 66 μg was injected in the same way. ④Liposome group:the same amount of liposome 200 μl was injected in the same way. ⑤NS group:the same amount of ns 200 μl was injected.Once every 3 days, 7 times in total. Two days after the end of the experiment, the nude mice were killed under general anesthesia. The weight of the tumor was measured and the size of the tumor was measured. The tumor was made into paraffin sections. The expression levels of VEGF, survivin, PCNA and CD34 were measured by immunohistochemistry. The microvessel density (MVD) and PCNA label index (PLI) of the tumor were calculated. Results: The weight, volume, VEGF and survivin protein, PCNA and CD34 expression of xenografts in nude mice were significantly decreased in the combined ASODN group. Conclusion:Co transfection of VEGF ASODN and Survivin ASODN can significantly inhibit the expression of VEGF and Survivin, and inhibit the growth and proliferation of Tca8113 tongue cancer cells. Reference | Related Articles | Metrics

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Comparison of the clinical value of routine blood test between venous blood and peripheral blood Tian Nannan Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (6): 463-465.   DOI: 10.3969/j.issn.2097-0005.2021.06.019 Abstract （606）      PDF （2180KB）（50）       Knowledge map    Save Objective: To compare the routine blood tests of venous blood and peripheral blood and analyze their clinical application value. Methods: Eighty patients with routine blood test collected in our hospital from February 2020 to October 2020 were selected and divided into two groups, control group and observation group, with 40 patients in each group. In the control group, peripheral blood was collected for routine blood test, while in the observation group, venous blood was collected for routine blood test, and the routine blood test of patients in the two groups was observed and compared. Results: The WBC in the observation group was significantly higher than that in the control group, and the difference was statistically significant.Compared with the control group, platelet (PLT), mean red blood cell hemoglobin content (MCHC), mean red blood cell volume (MCV), hemoglobin (Hb), red blood cell (RBC), specific volume of red blood cell (HCT) and other related indicators were all higher than the control group ( P<0.05). However, there was no statistically significant difference in MCH between the two groups( P>0.05).Blood routine test results showed that there was no statistical significance in RBC, WBC, MXD, PLT and other indicators detected within 3 h after venous blood collection compared with immediate test ( P>0.05). There was no statistically significant difference in RBC and WBC ( P>0.05),but MXD was significantly increased and PLT was significantly decreased ( P<0.05). Conclusion: In routine blood test, the effect of venous blood test is more stable, but the time of sending blood test should be strictly controlled. Reference | Related Articles | Metrics

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Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (6): 469-470.   DOI: 10.3969/j.issn.2097-0005.2021.06.021 Abstract （167）      PDF （6377KB）（49）       Knowledge map    Save Reference | Related Articles | Metrics

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Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (6): 471-475.   DOI: 10.3969/j.issn.2097-0005.2021.06.022 Abstract （187）      PDF （6381KB）（48）       Knowledge map    Save Reference | Related Articles | Metrics

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Construction of breast cancer protein risk prognosis model and identification of six key proteins Jian Li, Yuna Liang Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (10): 721-728.   DOI: 10.3969/j.issn.2097-0005.2021.10.001 Abstract （363）   HTML （750）    PDF （6457KB）（47）       Knowledge map    Save To optimize the prognosis assessment of breast cancer patients by developing proteomics signatures. The protein expression profiles and clinical information of 879 breast cancer （BC） patient samples were downloaded from the TCPA and TCGA databases， respectively. We performed univariate Cox regression， lasso regression and multivariate Cox regression analysis to identify prognostic proteins and establish a prognostic model. Then， we further analyzed the differential expression and survival of the prognostic model protein， and analyzed the predictive performance of the model risk score. In addition， we analyzed all proteins related to prognostic model proteins. Based on the 224 proteins in the TCPA database， we constructed a prognostic model containing 6 proteins （CASPASE7CLIVEDD198， NFKBP65_pS536， PCADHERIN， P27， X4EBP1_pT70， EIF4G）， and divided the patients into two groups according to the median of risk score. The overall survival rate was significantly different. Univariate and multivariate Cox regression analysis showed that the risk score was an independent prognostic factor for patients （P < 0.001）. In addition， the nomogram and ROC are used to demonstrate the predictive performance of the predictive model. The protein interaction relationship showed that the proteins CASPASE7CLEAVEDD198， PCADHERIN， X4EBP1_pT70 and EIF4G showed obvious correlation with other proteins （P < 0.001）. Studies have shown that six key proteins may be considered as new targets for BC treatment， and the protein risk model can be used to predict the overall survival of BC patients. Table and Figures | Reference | Related Articles | Metrics

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Clinical efficacy of tirofiban hydrochloride in the treatment of progressive stroke and transient ischemic attack Ma Fujing Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (6): 457-459.   DOI: 10.3969/j.issn.2097-0005.2021.06.017 Abstract （175）      PDF （2149KB）（46）       Knowledge map    Save Objective: To investigate the value of tirofiban hydrochloride in the treatment of progressive stroke and transient ischemic attack. Methods: 100 patients with Progressive stroke and transient ischemic attack from the Department of Neurology in June 2017 to June 2019 were analyzed, randomized digital table method was used to divide the observation group (control group, n=50) and hydrochloric acid treatment group (observation group, n=50) coagulant function index, nerve function score, the rate of adverse drug reactions, recurrent stroke were detected for comparison. Results: Before the treatment, there was no statistically significant difference between the two groups in coagulation function indexes APTT, PLT, PT and pecam-1 ( P＜0.05), no significant changes were observed in the APTT, PLT and PT indexes after the treatment ( P＞0.05); However, the pecam-1 index was significantly higher than that before treatment, and the observation group was significantly higher than that of the control group, with statistically significant differences ( P＜0.05).There was no difference in neurological function scores between the two groups before treatment ( P＞0.05). After treatment, the scores were all reduced, and there was a more significant reduction in the observation group, with statistically significant differences ( P＜0.05). Conclusion: Tirofebanja hydrochloride on the basis of conventional treatment can improve platelet aggregation, promote the recovery of nerve function, and reduce the risk of stroke recurrence without serious adverse reactions for progressive stroke and transient ischemic attack. Reference | Related Articles | Metrics

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Application of iron-based magnetic nanocarriers in tumor therapy Niu Panhong, Wang Yan, Liu Yingying, Zhang Jie, Zhang Shuping, Liu Sijin Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (5): 333-339.   DOI: 10.3969/j.issn.2097-0005.2021.05.003 Abstract （189）      PDF （6348KB）（42）       Knowledge map    Save Nano-drug delivery for tumor therapy has promoted the development of nanomaterials in the realm of biomedicine. Among them, iron-based magnetic nanomaterials have the advantages of good magnetism, excellent biocompatibility, and easy functional modification of the surface. As a nanocarrier, iron-based magnetic nanomaterials have received extensive attention and achieved rapid development in tumor therapy. Although there is a systematic understanding of Fe 3O 4 magnetic nanocarriers, the comprehensive and systematic understanding of iron-based magnetic nanoparticles carriers still needs to be strengthened. With the rapid development of tumor nano-drug delivery system design, it is urgent to systematically review the preparation process and mechanism of iron-based magnetic nanocarriers. In addition, ferroptosis, as a new type of cell death, receives much more attention in the role of occurrence and development of tumors and other chronic diseases, and the effect of iron-based magnetic nanoparticles on ferroptosis in tumor treatment applications remains to be elucidated. This review systematically introduces the development, types and preparation methods of iron-based magnetic nanometer drug-loading platforms, and detailedly summarizes the application of iron-based magnetic nanoparticles in the integration of tumor diagnosis and treatment, including magnetic field-assisted targeting, magnetic resonance imaging, and magnetocaloric therapy , and the mechanism of ferroptosis caused by the Fenton reaction is also iilustrated. Reference | Related Articles | Metrics

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Study on adenosine and the pathogenesis of epilepsy Mengqi Qi, Yuqin Lv, Wen Xu, Jingjun Zhang Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (9): 713-716.   DOI: 10.3969/j.issn.2097-0005.2021.09.017 Abstract （166）   HTML （10）    PDF （394KB）（41）       Knowledge map    Save Epilepsy is one of the common diseases of the nervous system. In recent years， the synthesis， metabolic pathways and functions of adenosine in the brain， as well as the pathogenesis and treatment of epilepsy on adenosine， have been one of the hot research topics at home and abroad. In this paper， the research progress is reviewed. Reference | Related Articles | Metrics

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Research progress of quadruple therapy for eradication of Helicobacter pylori Xinxiang Yu, Linlin Li, Ningning Peng, Weilin Wang, Binglong Li, Yongxiu Qi Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (8): 638-640.   DOI: 10.3969/j.issn.2097-0005.2021.08.020 Abstract （166）   HTML （6）    PDF （411KB）（39）       Knowledge map    Save Helicobacter pylori is a worldwide pathogen of human infection，which can cause gastrointestinal diseases and lead to the occurrence of gastric cancer. It has been listed as a kind of carcinogen by the InternationalAgency for Research on Cancer of the World Health Organization. Therefore， eradicating Hp infection is an inevitable problem for clinicians. With the continuous progress of Hp eradication treatment， the current clinical first-line treatment has developed into quadruple therapy. This study will focus on quadruple therapy，explore its therapeutic mechanism and the latest combined therapy，and provide a certain theoretical basis for clinical treatment. Reference | Related Articles | Metrics

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Alleviation effects of drinking hydrogen-rich water on the oral mucosal ulcer induced by 5-fluorouracil in rats Danya Zhang, Junli Xue, Boyan Liu, Geru Tao, Yunzhi Shi, Shucun Qin Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (9): 662-666.   DOI: 10.3969/j.issn.2097-0005.2021.09.005 Abstract （207）   HTML （13）    PDF （3085KB）（39）       Knowledge map    Save To investigate the alleviation effects of drinking hydrogen-rich water on the oral mucosal ulcer induced by 5-fluorouracil （5-FU） and its possible biological mechanism. Male SD rats were intraperitoneally injected with 5-FU （0.05 mg/g） for 2 consecutive days. On D3， rats were treated with NaOH particles or 20% acetic acid on buccal mucosa to establish oral ulcer model. From D5， the rats were given hydrogen-rich water， and the animals were sacrificed on the D10. The survival rates of rats in all experimental groups were counted， and the lesions of oral mucosa were measured by Image J software. The levels of TNF-α， IL-6 and IL-1β in serum were detected by ELISA， and the expressions of TNF-α， IL-6 and iNOS at buccal mucosa were detected by immunohistochemistry. Drinking hydrogen-rich water improved the survival rate of rats in the experimental groups. The damage area of ulcers produced by NaOH particles were larger than that caused by injection with 20% acetic acid （P < 0.05）， but there was no significant difference in the damage area of ulcers with drinking hydrogen-rich water or not （P > 0.05）. The serum levels of TNF-α， IL-6 and IL-1β of rats in model groups were significantly higher than those in the control group， while levels of the hydrogen-rich water group showed a downward trend compared with the model group. The expression of TNF-α and IL-6 at buccal mucosa were low in the healthy control and high in the model groups， while the expression in hydrogen-rich water groups showed a downward trend compared with the model group. The expression of iNOS at buccal mucosa was low in all groups. Molecular hydrogen alleviates oral mucosal ulcers induced by 5-FU in rats possibly through inhibiting oxidative stress-related inflammation. Table and Figures | Reference | Related Articles | Metrics

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Relationship to prognosis in changes of serum Ghrelin and NDAL levels in patients with acute pancreatitis Dai Wenling Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (6): 454-456.   DOI: 10.3969/j.issn.2097-0005.2021.06.016 Abstract （154）      PDF （2830KB）（38）       Knowledge map    Save Objective: To investigate the relationship between the levels of Ghrelin and neutrophil gelatinase-associated lipid lipocalin (NGAL) in peripheral blood and the prognosis of patients with acute pancreatitis (AP). Methods: To analyze the data of 75 acute pancreatitis patients admitted to Ningyang County First People's Hospital from October 2019 to September 2020, including 41 cases of acute mild pancreatitis(MAP group)and 34 cases of acute severe pancreatitis(SAP group).2ml venous blood was collected immediately after admission in the whole group, and the serum was separated by centrifugation, and stored in ultra-low temperature for backup test. The differences of serum Ghrelin and NGAL levels on the first day after admission were compared between the two groups. Results: The serum Ghrelin and NGAL levels of patients in the acute severe pancreatitis group were significantly higher than those in the acute mild pancreatitis group, with statistically significant differences ( P<0.001). Conclusion: The early serum levels of Ghrelin and NGAL in patients with acute pancreatitis are closely related to the severity of the disease, and the increased serum levels of Ghrelin and NGAL suggest a poor prognosis and deserve clinicians' attention. Reference | Related Articles | Metrics

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Clinical effect of endoscopic sinus surgery combined with drugs in the treatment of chronic sinusitis with nasal polyps Cao Hui, Liu Yong, Zheng Chengcai Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (6): 451-453.   DOI: 10.3969/j.issn.2097-0005.2021.06.015 Abstract （177）      PDF （2382KB）（34）       Knowledge map    Save Objective: To explore the clinical effect of endoscopic sinus surgery combined with drugs in the treatment of chronic sinusitis with nasal polyps. Methods: From March 2019 to March 2020, 63 patients with chronic sinusitis and nasal polyps in our hospital were selected as the research objects. According to the random number table method, the patients were randomly divided into control group (31 cases) and observation group (32 cases), in which the control group was treated with surgery alone, and the observation group was treated with surgery combined with drugs. The olfactory function of patients was analyzed and The complications and the total effective rate of treatment were compared between the two treatment modes. Results: The score of olfactory function in the observation group was lower than that in the control group ( P<0.05). The incidence of postoperative complications in the observation group was lower than that in the control group ( P<0.05). The total effective rate of the observation group was higher than that of the control group ( P<0.05). Conclusion: The curative effect of endoscopic sinus surgery combined with drugs in the treatment of chronic sinusitis with nasal polyps is better than that of surgery alone. At the same time, the olfactory function of the combined treatment is more ideal, and the risk of complications is less. Reference | Related Articles | Metrics

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Diagnosis and treatment of 21 cases with post necrotizing enterocolitis strictures in infants Liu Wei Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (6): 466-468.   DOI: 10.3969/j.issn.2097-0005.2021.06.020 Abstract （155）      PDF （2375KB）（34）       Knowledge map    Save Objective: To investigate the diagnosis and treatment of neonatal necrotizing enterocolitis (NEC) posterior bowel stricture. Methods: Data of 21 patients with NEC posterior bowel stenosis admitted to our hospital from February 2015 to September 2020 were retrospectively analyzed, and the growth and development of the children were followed up.In accordance with the Bell stage, 5 cases in periodⅠ, 14 cases in periodⅡ and 2 cases in period Ⅲ among the 21 cases. All the children were preoperatively examined by orthographic abdominal X-ray, and 19 cases were simultaneously examined by high-frequency ultrasound.All the 21 cases received surgical treatment after diagnosis. Results: The average consultation time for NEC's posterior intestinal stricture was 29.5 days.Abdominal elevations showed inflating intestinal curvature, and 20 cases presented stepped liquid and gas plane.High-frequency ultrasound examination showed extensive dilatation and effusion of the intestine.Most of the stenosis sites were found in ascending colon, transverse colon and descending colon.Twenty-one cases had intestinal anastomosis Ⅰ period bowel resection, were cured after operation and discharged, and had no deaths.Among 18 cases followed up for a long time, 17 cases had normal diet and development, and 1 case was cured and discharged after intestinal adhesion release due to adhesion obstruction. Conclusion: Colon is more common in NEC posterior bowel stricture.Preoperative abdominal X-ray and high frequency ultrasound are the first methods.It is advisable to narrow bowel resection in the treatment for intestinal anastomosis in periodⅠ. Reference | Related Articles | Metrics

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Research on the progress in radiotherapy of lung cancer in 2020 Liu Jie, Li Xuanzong, Yu Jinming Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (5): 321-326.   DOI: 10.3969/j.issn.2097-0005.2021.05.001 Abstract （194）      PDF （5232KB）（34）       Knowledge map    Save As one of the main treatment methods, radiotherapy plays a very important role in all the stages for lung cancer. Outstanding results were achieved in radiotherapy in lung cancer during 2020. Radiotherapy combining with immunotherapy is still the research hot topic. Radiotherapy combined with targeted therapy, and the choice of radiotherapy dose and technology have attracted much attention. This article reviews the important research progress of radiotherapy in the treatment of lung cancer in 2020. Reference | Related Articles | Metrics

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Effect of Tongfuxiaoduyin enema and external application combined with esomeprazole on intestinal mucosal barrier function and inflammatory state in patients with severe acute pancreatitis Zhongli Wei Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (8): 602-605.   DOI: 10.3969/j.issn.2097-0005.2021.08.010 Abstract （217）   HTML （2）    PDF （481KB）（34）       Knowledge map    Save To explore the effect of Tongfuxiaoduyin enema and external application combined with esomeprazole in the treatment of severe acute pancreatitis，and to explore the effect of it on intestinal mucosal barrier function and inflammatory state of patients. Ninety patients with severe acute pancreatitis were randomly divided into control group and observation group，with 45 patients in each group. The control group was treated with esomeprazole，and the observation group was additionally treated with Tongfuxiaoduyin enema and external application on the basis of the treatment in the control group. The treatment lasted for 7 days. The severity of the disease， intestinal mucosal barrier function and inflammatory status were compared between the two groups. Compared with the control group，the Apache score and Balthazar CT score in the observation group were significantly decreased after treatment，with statistical significance （P < 0.05）. Compared with the control group，the indexes of intestinal mucosal barrier function （D-lactic acid，AMS，DAO） decreased more significantly in the observation group after treatment，with statistical significance （P < 0.05）. Compared with the control group，the serum inflammatory state related indexes of SICAM-1 and MCP-1 in the observation group decreased more obviously after treatment，while the content of MFG-E8 increased more obviously，the difference was statistically significant （P < 0.05）. Tongfuxiaoduyin enema and external application combined with esomeprazole is more conducive to reduce the severity of patients with acute pancreatitis，promote the recovery of intestinal mucosal barrier function and improve the prognosis，whose effect may be related to the inhibition of SICAM-1，MCP-1 expression，promotion of the secretion of MFG-E8 to reduce inflammatory response and protection of the intestinal barrier，which is worthy of clinical application. Table and Figures | Reference | Related Articles | Metrics

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Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (5): 358-361.   DOI: 10.3969/j.issn.2097-0005.2021.05.006 Abstract （165）      PDF （3301KB）（32）       Knowledge map    Save Reference | Related Articles | Metrics

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Attenuation of neurological deficit after SAH and the promotion of microglia polarization toward M2 phenotype in mice by the novel Nrf2 activator CDDO-EA Qiwei Dong, Huayang Zhao, Shihao Ren, Pengjie Zhang, Yajun Hou, Leilei Mao Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (9): 645-650.   DOI: 10.3969/j.issn.2097-0005.2021.09.002 Abstract （275）   HTML （39）    PDF （3636KB）（32）       Knowledge map    Save To investigate the effects of CDDO-EA （CDDO-ethylamide）， a novel Nrf2 related factor 2 （Nrf2）?， on neurological deficit and microglia polarization after subarachnoid hemorrhage （SAH） in mice. Thirty healthy C57BL/6 mice were randomly divided into sham group， SAH + PBS group and SAH + CDDO-EA （50 μg） group. The SAH model was made by middle cerebral artery puncture， and the mice were sacrificed 3 days after operation for neurological function score. Brain tissue was collected for frozen sections and the M2-type microglia cells were labeled with CD206/Iba1 by immunofluorescence double staining. The cerebral cortex of each group was collected 48 h after operation to detect the expression of CDDO-EA by Western blot. Compared with PBS control group， the expression of Nrf2 in brain of 50 μg and 100 μg treatment groups was significantly increased （P < 0.05）.The mice received PBS or CDDO-EA（50 μg） after surgery by intraperitoneal injection. The neurological function score was evaluated 72 h after surgery. SAH mice had obvious neurological dysfunction， but the neurological function was improved after CDDO-EA treatment （P < 0.05）. In addition， the numberof M2 microglia in cerebral cortex and hippocampus after CDDO-EA treatment significantly increased than that in vehicle control group. CDDO-EA treatment attenuates neurological deficits after SAH， which may be achieved by up-regulating the expression of Nrf2 and promoting microglia polarization toward M2 phenotype. Table and Figures | Reference | Related Articles | Metrics

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Analysis of the clinical value of MRI routine sequence combined with DWI and DCE-MRI sequence in the diagnosis of ovarian tumors Liugeng Qu, Xiaojing Wang Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (8): 588-591.   DOI: 10.3969/j.issn.2097-0005.2021.08.006 Abstract （229）   HTML （2）    PDF （929KB）（31）       Knowledge map    Save To analyze its clinical valuein ovarian tumor MRI routine scanning combined with DWI and DCE-MRI sequences. 191 patients with ovarian tumorsincluding 102 benign （benign group） and 89 malignant （malignant group） treated in our hospitalfrom February 2019 to February 2020 were selected，all underwent MRI scan， DWI， DCE-MRI sequence scan，and the DWI parameters， TIC image types， DCE-MRI parameters and diagnosis results of the two groups of patientswere compared. The ADC values of the solid and cystic parts of the benign group were significantly higher than those of the malignant group （P < 0.05）； the TIC image of the benign group was mainly type Ⅰ （91.18%）， and the malignant group was mainly type Ⅲ （86.52%）. The difference was obvious （P < 0.05）； the Ktrans，Kep， Ve and other DCE-MRI parameters of the benign group were significantly lower than those of the control group （P <0.05）； There was no significant difference in the degreeof the sensitivity， specificity and accuracy of the MRI routine sequence combined with DWI and DCE-MRI （P > 0.05）. MRI routine sequence combined with DWI and DCE-MRI sequence can achieve good diagnostic results，and it is worthy of promotion. Table and Figures | Reference | Related Articles | Metrics

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Application of closed drainage with negative pressure combined with perforator flap transplantation in the treatment of soft tissue defect of foot and ankle Xinqiang Jiang, Fang Zhang Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (8): 617-620.   DOI: 10.3969/j.issn.2097-0005.2021.08.014 Abstract （215）   HTML （2）    PDF （428KB）（31）       Knowledge map    Save To observe the effect of negative pressure closed drainage （VSD） combined with perforator flap transplantation in the treatment of ankle soft tissue defect. Sixty cases of soft tissue defect of the ankle were from our hospital from May 2018 to May 2020. There were 30 cases in the observation group （VSD + perforator flap transplantation） and the control group （traditional wound + perforator flap transplantation）respectively.Treatment related indicators，ankle function score （Kphoed），pain score （VAS），treatment effect，and incidence of complications were compared between the two groups before and after treatment. Compared with the control group，the duration of surgery，antibiotic application and hospitalization in the observation group was shorter，and the number of dressing changes and the area of skin flap incision in the observation group were less than those in the control group，with statistically significant differences （P < 0.05）；After treatment，Kphoed scores of patients in both groups were improved，while VAS scores were decreased，which were better than those before treatment， and statistically significant （P < 0.05）；The total effective rate of 93.3% in the observation group was higher than that of 70.0% in the control group，which was statistically significant （P < 0.05）； In the comparison of complication rates between the two groups， the observation group was lower than the control group （6.7% vs 26.7%） （P < 0.05）. VSD combined with perforator flap transplantation plays a significant role in improving patients' psychological status and quality of life，which is conducive to reduce the occurrence of adverse events，and can be widely promoted in clinical practice. Table and Figures | Reference | Related Articles | Metrics

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The diagnostic value of peripheral blood NLR， PLR and SII in thyroid nodules Zhongwei Fang, Xianwei Zhou, Zhiqiang Cao, Yanfang Song, Gengpu Ren Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (9): 675-679.   DOI: 10.3969/j.issn.2097-0005.2021.09.008 Abstract （184）   HTML （9）    PDF （813KB）（29）       Knowledge map    Save To investigate the values of the differential diagnosis of peripheral blood neutrophil/lymphocyte ratio （NLR）， platelet/lymphocyte ratio （PLR） and systemic immune inflammation index （SII） in healthy people， benign and malignant thyroid nodules. 172 patients with benign thyroid nodules and 285 patients with malignant nodules diagnosed in the Second People's Hospital of Liaocheng from January 2017 to October 2019 were selected as the research objects， and 199 patients with health examinations in the hospital during the same period were selected as the control group， the preoperative peripheral blood neutrophil count， lymphocyte count and platelet count were recorded， and NLR， PLR and SII were calculated， and NLR， PLR and SII in healthy people， benign nodules， and malignant nodules were compared by t test for the difference between groups， the receiver operating characteristic curve （ROC curve） was used to determine the critical values of NLR， PLR， and SII in different groups. Except that there was no statistically significant difference between NLR and SII in the comparison of benign and malignant nodules （group A vs group B）， the three indicators （NLR， PLR， SII） in the other three groups were compared with each other， and the differences were statistically significant （P < 0.05）. The area of AUC was between 0.72 and 0.8. The sensitivity of NLR， PLR， and SII in the identification of healthy people and benign thyroid nodules was 85.9%， 92.5%， and 81.9% respectively. In the detection of healthy people and malignant thyroid nodules， the sensitivity in the discrimination was 70.4%， 81.9%， and 74.1%， respectively. The discrimination specificities in the benign nodule group and the malignant nodule group were 85.6%， 69.5%， and 83.2%， respectively， and the significance was significant. The detection of NLR， PLR， and SII has a certain value to distinguish between healthy people and benign nodules and healthy people and thyroid nodules， but it is not significant to distinguish between benign and malignant nodules. Table and Figures | Reference | Related Articles | Metrics

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Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (10): 775-780.   DOI: 10.3969/j.issn.2097-0005.2021.10.012 Abstract （44）   HTML （5）    PDF （1208KB）（28）       Knowledge map    Save Table and Figures | Reference | Related Articles | Metrics

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Prokaryotic expression of enterovirus 71 type VP1 protein and its application in high throughput antibody screening Ke Cui, Yaru Kong, Chuanmin Ma, Zhenjie Zhang, Weifeng Shi, Chuansong Quan Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (9): 641-644.   DOI: 10.3969/j.issn.2097-0005.2021.09.001 Abstract （111）   HTML （9）    PDF （1394KB）（27）       Knowledge map    Save Enterovirus 71 （EV71） is the main pathogen causing severe hand， foot and mouth disease （HFMD）， however， there is no effective antiviral drugs available. To screen mouse specific B cells expressing antibodies against EV71 by using EV71 VP1 protein as an antigen. The codon-optimized EV71 VP1 gene was cloned into pET-21a （+） expression vector and transformed into E. coli. BL21-DE3. By optimizing protein expression and renaturation conditions， the expressed protein was confirmed by SDS-PAGE and Western blot and its antigenicity was also tested. Using purified protein as antigen， B lymphocytes of mice immunized with inactivated， and EV71 viruses were sorted by flow cytometry for the preparation of monoclonal antibody. The EV71 VP1 gene was stably expressed in the form of inclusion body in the E. coli. The purified protein showed good immunogenicity， and there was a significant difference between the B cells of immunized and unimmunized BALB/c mice when screened using the expressed protein. The recombinant EV71 VP1 protein with good immunogenicity is successfully obtained and combined with flow cytometry， it can be used for detection and sorting of EV71 specific B cells. Table and Figures | Reference | Related Articles | Metrics

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Research directions worth paying attention to in tumor radiotherapy in the immune era Liu Chengxin, Li Baosheng Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (5): 327-332.   DOI: 10.3969/j.issn.2097-0005.2021.05.002 Abstract （86）      PDF （5346KB）（25）       Knowledge map    Save Immunotherapy plays an increasingly important role in cancer treatment, especially immune checkpoint inhibitor therapy. However, due to low immunotherapy efficiency, a synergistic anti-tumor response may be produced through combined application with traditional tumor treatment methods. Among them immune combined radiotherapy is highly anticipated. Radiotherapy can directly or indirectly act on tumor cells by regulating the immune response in a variety of ways, such as inducing immunogenic cell death or changing the immune cell infiltration in the tumor microenvironment. Immunotherapy can also affect the effect of radiotherapy by regulating the infiltrating immune cells in the tumor microenvironment and the normalization of blood vessels. This article focuses on the interaction and theoretical basis between immunotherapy and radiotherapy, then analyzes in detail the effects of different doses and timely radiotherapy on immune response, and finally summarizes the problems and future development directions of radiotherapy under the influence of immunotherapy. Reference | Related Articles | Metrics

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Meta-analysis of Shugan Jieyu Capsule combined with Escitalopram in the treatment of depression Miao Shuai, Jiao Min, Xu Xiaoyan Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (5): 390-395.   DOI: 10.3969/j.issn.2097-0005.2021.05.012 Abstract （180）      PDF （4769KB）（25）       Knowledge map    Save Objective: To systemically evaluate shugan jieyu capsule combined with escitalopram efficacy and safety in the treatment of depression,and to provide reference for guiding its clinical application.Methods: Databases, including CNKI, WanFang, VIP and PubMed Data, were retrieved by two assessors independently so as to retrieve randomized controlled trials(RCTs)on Shugan Jieyu Capsule combined with escitalopram in treating depression.Data were extracted according to inclusion and exclusion criteria, and RevMan 5.3 software was used for quality evaluation and meta analysis of the included literature. Results: A total of 836 subjects were included in 10 RCTs. Meta analysis results showed that the experimental group (Shugan Jieyu Capsule combined with escitalopram) had better clinical efficacy, and the total response rate [OR=3.15,95%CI(2.08,4.79),P<0.000 01] was statistically significant compared with the control group (escitalopram alone). In trems of HAMD score before and after treatment, [MD=-3.03,95%CI(-4.59,-1.47),P=0.000 1], the difference was statistically significant.Results of adverse reaction incidence [OR=0.74,95%CI(0.50,1.08),P=0.12] showed that the difference was not statistically significant.Conclusion: Shugan Jieyu Capsule combined with escitalopram has a good effect in the treatment of depression, and can significantly reduce patients' HAMD score, which has a good application prospect. Reference | Related Articles | Metrics

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Research progress of intestinal type 3 innate lymphoid cells Wang Xin, Chen Zhengtao, Tang Hua Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (5): 347-357.   DOI: 10.3969/j.issn.2097-0005.2021.05.005 Abstract （215）      PDF （9485KB）（24）       Knowledge map    Save Innate lymphoid cells (ILCs) are a new class of innate immune cells that are an important component of the immune system and were discovered more than a decade ago. They play an important role in regulating the immune response and homeostasis. According to the different transcription factors expressed and cytokines secreted by ILCs, they can be divided into three distinct groups: ILC1s, ILC2s and ILC3s. Among them, ILC3s mainly exist in the intestinal tract, and maintain the immune homeostasis of the intestinal mucosa by participating in promoting the formation of lymphatic organs, maintaining the integrity of the epithelial barrier, regulating intestinal inflammation, and promoting antibacterial response. The author of this article reviews the development, characteristics and pathophysiological functions of ILC3s in the immune system of the intestine and summarizes the main research progress of ILC3s. Reference | Related Articles | Metrics

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Study on the difference in expression of CYP2C19 and GPⅡb/Ⅲa between patients with familial LAA atherosclerotic cerebral infarction and those with sporadic LAA cerebral infarction Hao Rui, Xu Shuangshuang, Gao Min, Wang Yanhua, Lv Yuqin, Wang Na, Zhang Jingjun Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (5): 385-389.   DOI: 10.3969/j.issn.2097-0005.2021.05.011 Abstract （170）      PDF （3949KB）（23）       Knowledge map    Save Objective: To investigate the differences in expression of ADP, CYP2C19, P2Y12 receptors and GPⅡb/Ⅲa between patients with familial atherosclerotic (large-artery atherosclerosis, LAA) cerebral infarction and those with sporadic LAA cerebral infarction. Methods: Twenty-five patients with familial aggregated LAA cerebral infarction and sporadic LAA cerebral infarction were collected as family group and sporadic group respectively .Twenty-five healthy people were selected as control group. ADP protein expression, CYP2C19 gene, P2Y12 gene G52T locus and GPⅡb/Ⅲa protein expression were detected respectively. Results: There was significant difference in ADP expression between the family group and the control group; There was no significant difference between the family group and the sporadic group and between the sporadic group and the control group; Compared with the control group, the expression of CYP2C19 genes in the family group was significantly down-regulated, and the difference was statistically significant; Compared with the control group, the expression of CYP2C19 genes in the sporadic group had no significant difference in gene expression; The G52T loci of P2Y12 gene were significantly different between family group, sporadic group and control group. The expression of GPⅡb/Ⅲa in family group and control group was significantly up-regulated, and the difference was statistically significant. The difference in the expression of GPⅡb/Ⅲa. between family group and sporadic group and between sporadic group and control group was not significant; After 3 months of treatment, mRS scores of the family group and the sporadic group were compared, and the difference between the two groups was statistically significant. Conclusion: There are significant differences in ADP protein expression, CYP2C19 gene, P2Y12 gene G52T locus and GPⅡb/Ⅲa protein expression between family group and sporadic group. Reference | Related Articles | Metrics

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The expression of Orexin-A neurons in the hypothalamus of rats after sleep deprivation and its effect on learning and memory Ni Liyan, Tang Jiyou Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (5): 380-384.   DOI: 10.3969/j.issn.2097-0005.2021.05.010 Abstract （93）      PDF （6148KB）（23）       Knowledge map    Save Objective: To investigate the expression of Orexin-A neurons in the hypothalamus of rats after sleep deprivation and its effect on learning and memory.Methods: Healthy adult male Wistar rats were randomly divided into cage control (CC), treadmill control (TC), sleep deprivation (SD) group. 72h total sleep deprivation was performed by intermittent treadmill. The expression of Orexin-A was observed by immunohistochemistry, and the spatial learning and memory ability was tested by Morris water maze test. Results: Compared with TC, the number of Orexin-A positive cells in hypothalamus of SD rats was significantly increased ( P<0.01). There was no significant difference between TC and CC group ( P>0.05). Compared with TC group, the escape latency and the times of crossing platform quadrant in SD group were significantly longer ( P<0.01), and there was no significant difference between TC group and CC group ( P>0.05). Conclusion: Sleep deprivation may impair spatial learning and memory in rats by inducing overexpression of Orexin-A in hypothalamus. Reference | Related Articles | Metrics

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Survey of anxiety and blood pressure control behavior of patients with hypertension in some rural areas of Anhui Province Jianjian Jiang, Rong Liu, Xingrong Shen, Anan Chen, Ting Zhu, Zhimin Xiao, Debin Wang Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (8): 561-567.   DOI: 10.3969/j.issn.2097-0005.2021.08.001 Abstract （225）   HTML （16）    PDF （547KB）（23）       Knowledge map    Save To understand the status of anxiety and blood pressure control behavior of patients with hypertension in parts of rural areas，andto analyze the relations among the anxiety，blood pressure control behavior and blood pressure. 277 patients with hypertension were selected from three administrative villages of Jieshou City in Anhui Province，self-made questionnaire and self-rating anxiety scale（SAS） were used to conduct face to face survey. The mean of the total score of anxiety scale was （38.27 ± 11.059），the detection rate of anxiety disorders was 14.9%.Comprehensive classification of blood pressure，increase exercise， age group and BMI classification were positively correlated with the total anxiety scores or dimension scores（P < 0.05），but dietcontrol and presence of complications were negatively correlated with the total anxiety scores or dimension scores（P < 0.05）.Dysfunction of the body，dietcontrol，smokingcontrol， moodregulation，going out or not and age group of hypertension diagnosis were positively correlated with comprehensive classification of blood pressure or diastolic and systolic blood pressure levels（P < 0.05），but mood disorder，mixed cross symptom，drinking control and age group were negatively correlated withcomprehensive classification of blood pressure or diastolic and systolic blood pressure levels（P < 0.05）.Comprehensive classification of blood pressure was the hinder factor of control diet（OR = 1.537 or 1.543）.The total anxiety scores was the promotion factor of control diet〔OR（95%CI） = 0.959（0.919～1.000）〕，but was the hinder factor of increasing exercise〔OR（95%CI） = 1.032 （1.004～1.060）〕.Mixed cross symptom is the promotion factor of smokingcontrol〔OR（95%CI）= 0.805（0.683～0.949）〕and drinkingcontrol〔OR（95%CI） = 0.823（0.698～0.971）〕. Anxiety affects blood pressure， and its behaviorcontrol and blood pressure also affect anxiety and behaviorcontrol.In order to control blood pressure better， we should relieve patients' bad mood and promote the implementation of blood pressure control behavior. Table and Figures | Reference | Related Articles | Metrics

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Effect of bilateral uterine artery embolization combined with hysteroscopic electrotomy on cesarean scar pregnancy （CSP） Lijun Hu Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (8): 613-616.   DOI: 10.3969/j.issn.2097-0005.2021.08.013 Abstract （186）   HTML （2）    PDF （614KB）（23）       Knowledge map    Save To study and analyze the therapeutic effect of BUAE （bilateral uterine artery embolization） combined with traditional cervicotomy and BUAE combined with hysteroscopic electrotomy in patients with cesarean section scar pregnancy. 126 patients with cesarean section scar pregnancy received by our hospital from March 2019 to March 2020 were taken as observation subjects.According to the double-blind randomized control principle，the study group was divided into two groups， one group was the conventional group （BUAE combined with traditional palace clearance） and the other group was the experimental group （BUAE combined with hysteroscopic electrotomy）.Each group had 63 cases.The surgical efficacy of the two groups was compared. The success rate of the study group was higher than that of the conventional group （P < 0.05）.The amount of surgical blood loss in the study group was lower than that in the conventional group， and the operation time， menstrual cycle recovery time，serum-HCG（a subunit of human chorionic gonadotropin） recovery time and hospitalization time were shorter than those in the conventional group （P < 0.05）.Postoperative complications in the study group were less than those in the conventional group （P < 0.05）. Compared with traditional hysterectomy combined with BUAE， hysteroscopy combined with BUAE for cesarean section scar pregnancy patients has many advantages， such as outstanding efficacy， low blood loss， fewer postoperative complications， high safety and fast recovery， which is worth popularizing in clinic. Table and Figures | Reference | Related Articles | Metrics

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Optimization of cellulase production process for solid-state fermentation of stem and leaf from Salvia miltiorrhiza by endophytic fungus LZY9 Wang Yongdi, Qiu Mengjiao, Zhang Xiaohua, Wang Xin, Zhang Zhihao, Li Yanling, Chang Zhengyao Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (5): 375-379.   DOI: 10.3969/j.issn.2097-0005.2021.05.009 Abstract （89）      PDF （6295KB）（22）       Knowledge map    Save Objective: To further develop and utilize Salvia miltiorrhiza resources and optimize the technological conditions for cellulase production by solid-state fermentation. Methods: Using endophytic fungus LZY9 of Salvia miltiorrhiza as strain, on the basis of single factor experiment, response surface methodology was used to optimize the three factors of water content, inoculation amount and culture days of cellulase produced by solid fermentation of endophytic fungus LZY9, and the cellulase activity was determined by CMC sacylation method. Results: The optimum fermentation conditions of endophytic fungus LZY9 were as follows: water content was 5.0%, inoculum quantity was 60%, culture days was 5 days. Under the optimized conditions, the enzyme activity was 13.417 IU/g. The analysis of variance showed that there was no misfit factor in this experiment, and the three factors had obvious influence on the experimental results. Conclusion: This method is a new method for comprehensive utilization of stem and leaf wastes of Salvia miltiorrhiza on the ground. Reference | Related Articles | Metrics

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Effect of cisplatin intraperitoneal hyperthermic perfusion chemotherapy on apoptosis of human gastric cancer cell lines and its mechanism Wang Fabao, Xia Zeliang, Chu Liang, Zhou Shaobo, Xu Zhaolong, Jiang Lei, Shan Erbo, Zhou Chaoyi Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (5): 396-400.   DOI: 10.3969/j.issn.2097-0005.2021.05.013 Abstract （151）      PDF （6166KB）（22）       Knowledge map    Save Objective: To preliminarily explore the effect of intraperitoneal hyperthermic perfusion of cisplatin on the apoptosis of human gastric cancer cell line (SGC-7901) and its mechanism.Methods: The four groups divided in the experiment were the control group, cisplatin group, hyperthermia group and hyperthermia chemotherapy group. CCK-8 was used to detect cell viability in each group, Hoechst staining method was used to detect cell apoptosis, flow cytometry was used to detect cell apoptosis rate, and Western blot was used to detect the expression levels of Caspase-3, Bcl-2, Bax and Bid proteins. Results: Compared with the control group, the cell viability of the cisplatin group, the hyperthermia group and the thermochemotherapy group was significantly reduced ( P<0.01), the apoptosis rate was significantly increased ( P<0.01), and the Caspase-3, Bax and Bid proteins were significantly increased High ( P<0.05 or P<0.01), Bcl-2 expression was down-regulated ( P<0.01). Compared with the cisplatin group and the hyperthermia group, the cell viability of the thermochemotherapy group was significantly reduced ( P<0.05), the apoptosis rate was significantly increased ( P<0.05), and the Caspase-3, Bax and Bid proteins were significantly increased ( P<0.05), the expression of Bcl-2 was significantly down-regulated ( P<0.05). There was no significant difference between the cisplatin group and the hyperthermia group ( P>0.05). Conclusion: Cisplatin intraperitoneal hyperthermic perfusion chemotherapy can promote the apoptosis of SGC-7901 cells by promoting the expression of Caspase-3, Bax and Bid and down-regulating Bcl-2. Reference | Related Articles | Metrics

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The value of serum and cerebrospinal fluid S-100β,NSE,MBP combined with slCAM-1 in evaluating the neurological function of children with viral encephalitis Liu Baoru, Zhou Jiangchao Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (6): 439-441.   DOI: 10.3969/j.issn.2097-0005.2021.06.011 Abstract （165）      PDF （2275KB）（22）       Knowledge map    Save Objective: To explore the value of serum and cerebrospinal fluid S-100β, NSE, MBP combined with slCAM-1 in evaluating the neurological function of children with viral encephalitis. Methods: A total of 130 children with viral encephalitis who were admitted to the Department of Neurology in our hospital from June 2018 to December 2019 were selected. According to the clinical manifestations, they were divided into the barrier-free group (without central nervous function barrier, 68 cases) and the barrier group (with central nervous system dysfunction, 62 cases). The evaluation of the values of S-100β, NSE, MBP, slCAM-1 levels in the children with neurological function in each group was made. Results: The levels of S-100β, NSE, MBP and slCAM-1 in serum and cerebrospinal fluid of the barrier group were higher than those of the barrier group ( P<0.05). The sensitivity and specificity of the four combined tests were higher than those of the individual tests( P<0.05). Conclusion: Serum and cerebrospinal fluid S-100β, NSE, MBP combined with slCAM-1 are closely related to the neurological function of children with viral encephalitis.The detection of the levels of four items in serum and cerebrospinal fluid is helpful for the assessment of the neurological function of children. Reference | Related Articles | Metrics

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Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (3): 232-233.   DOI: 10.3969/j.issn.1004-7115.2021.03.018 Abstract （75）      PDF （1135KB）（22）       Knowledge map    Save Reference | Related Articles | Metrics

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Meta-analysis of the curative effect of Gandou Decoction in the treatment of hepatolenticular degeneration Meiling Chen, Hongling Xiao, Qian Wang, Yunjiu Xiao, Yiting Zhang Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (8): 573-578.   DOI: 10.3969/j.issn.2097-0005.2021.08.003 Abstract （198）   HTML （3）    PDF （4224KB）（22）       Knowledge map    Save To evaluate the efficacy of Gandou Decoction in the treatment of hepatolenticular degeneration through Meta analysis. Theonlinesearch for Gandou Decoction for treatment of liver peas and randomized controlled trial study of nucleus degeneration was made in the Chinese Journal Full-text Database （CNKI）， VIP Chinese Science and Technology Journal Full-text Database （VIP）， Chinese Academic Journals Database （Wanfang），Chinese Biomedical Literature Database （CBM），Pubmed in combination with manual search. After selecting the literature according to the inclusion and exclusion criteria，data extraction and quality evaluation，the RevMan5.3 software provided by the Cochrane Collaboration was used for meta-analysis. A total of 10 RCT studies were included，including 639 patients. The results of Meta analysis showed that Gandou Decoction was better than the western medicine group in terms of total effective rate，improvement of liver function，and 24 h urinary copper excretion in the treatment of hepatolenticular degeneration. The treatment principle of hepatolenticular degeneration is to drive copper and protect the liver. According to the results of the study，Gandou Decoction has thedefinite effect of removing copper and protecting the liver. However，the current research evidence is still insufficient，and further studies with larger samples and higher quality are needed in the future in order to clarify the therapeutic effect of Gandou Decoction. Table and Figures | Reference | Related Articles | Metrics

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Effect of combined anesthesia of remifentanil and sevoflurane in elderly patients undergoing laparoscopic surgery He Youli, Kong Jing Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (6): 442-444.   DOI: 10.3969/j.issn.2097-0005.2021.06.012 Abstract （176）      PDF （2565KB）（21）       Knowledge map    Save Objective: To observe the effect of combined anesthesia of remifentanil and sevoflurane in elderly patients undergoing laparoscopic surgery. Methods: A total of 90 elderly patients undergoing laparoscopic surgery in our hospital from January 2019 to June 2020 were selected and divided into control group ( n=45) and observation group ( n=45) according to random number table method.The control group was anesthetised with remifentanil, and the observation group was anesthetised with combined sevoflurane and remifentanil.SOMCT score, Steward resuscitation score, extubation, resuscitation time and adverse reactions were compared between the two groups at each time point. Results: The comparison of SOMCT scores at 1h and 3h after extubation between the two groups showed that the observation group was better than the control group ( P<0.05), which was statistically significant.Comparison of Steward recovery scores at extubation and 15min extubation between the two groups showed that the observation group was better than the control group ( P<0.05), which was statistically significant.The comparison of extubation and recovery time between the two groups showed that the observation group was better than the control group ( P<0.05), which had statistical value. Comparison of adverse reaction rate between the two groups showed that observation group was better than control group ( P<0.05), which was statistically significant. Conclusion: Combined anesthesia of sevoflurane and Remifentanil has a significant effect on postoperative short-term cognitive function in elderly patients undergoing laparoscopic surgery.In other words, it has little influence on postoperative short-term cognitive function, can improve the quality of recovery, and promote postoperative recovery of patients with fewer adverse reactions. Therefore, it is worth promoting research. Reference | Related Articles | Metrics

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Study on HPLC fingerprint of Mengshiguntan pills Xinhong Zhao, Shan Fan, Yongxiu Qi Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (7): 492-496.   DOI: 10.3969/j.issn.2097-0005.2021.07.003 Abstract （213）   HTML （2）    PDF （1778KB）（21）       Knowledge map    Save To establish a method for fingerprint analysis of Yishi guntan pills. A thermo ODS hypersil column（4.6 mm × 250 mm， 5 μm）was used with methanol-0.1% phosphoric acid solution as the mobile phase， the flow rate was 1.0 mL·min-1， the detection wavelength was 254 nm， the column temperature was 30 ℃， and baicalin was used as the internal reference peak. HPLC fingerprints of 10 batches of Yishi guntan pills were established. Similarity evaluation software of chromatographic fingerprints of Chinese medicine was used to evaluate the similarity of 10 batches of preparations， and the characteristic peaks were identified. The specificity， precision， repeatability and stability of the HPLC fingerprint of Yishi guntan pills were good， and the similarity of 10 batches of preparations was greater than 0.9. The established method is simple， accurate and specific. The quality of Yishi guntan pills is controlled by multi index components， which provides a reliable basis for the overall quality evaluation of Yishi guntan pills. Table and Figures | Reference | Related Articles | Metrics

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Prevalence of cardiovascular comorbidities in severe acute respiratory syndrome coronavirus 2（SARS-CoV-2）： a systematic review and Meta-analysis Jianhua Chen, Ruimin Tian, Lijun Luo, Chunlei Yu Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (7): 525-533.   DOI: 10.3969/j.issn.2097-0005.2021.07.010 Abstract （40）   HTML （2）    PDF （4270KB）（21）       Knowledge map    Save To evaluate the prevalence of major comorbidities and death cases and to identify potential high risks disease associated with severe acute respiratory syndrome type 2（SARS-CoV-2） infection by meta-analysis. Literatures search was conducted using Wanfang Database， CNKI， CQVIP Database and Chinese Biomedical Literature Database（CBM） from 1 December 2019 to 3 May 2020. Meta-analysis of hypertension and diabetes in patients with coronavirus disease was conducted by using Open Meta-Analyst version 10.10. Forest plots were used to illustrate the prevalence of the hypertension and diabetes. A total of 4814 patients with SARS-CoV-2 infection were included. The rates of hypertension and diabetes were 19.6% and 9.3%， respectively. The prevalence rates of hypertension and diabetes were 23.2% and 10.9%， respectively according to the summary of the 2018 Report on Cardiovascular Disease in China. In comparion of our data with that of the 2018 Report， we concluded that the prevalence of hypertension and diabetes among people infected with the SARS-CoV-2 is roughly the same as that in the general population， but the morbidities of the hypertension and diabetes were 40.0% and 21.5%， respectively. More than 70.0% of patients was over 60 years old. It can not be said that the patients with hypertension and diabetes are more susceptible to SARS-CoV-2 infection， but hypertension， diabetes and > 60 years old can increase the risk of the morbidities in patients with SARS-CoV-2 infection. The survival rate of patients with SARS-CoV-2 disease can be improved if a public health vaccination strategy is developed for those with these chronic diseases. Table and Figures | Reference | Related Articles | Metrics

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Research progress of fecal bacteria transplantation in clinical application of intestinal diseases Zilong Xu, Zhijun Geng, Jing Li Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences    2021, 42 (7): 551-555.   DOI: 10.3969/j.issn.2097-0005.2021.07.014 Abstract （195）   HTML （2）    PDF （545KB）（21）       Knowledge map    Save The microbial community in the human intestine is very important to the function and health of the body. The changes in its ecology have been involved in the pathogenesis of many diseases. For this reason， novel treatment options based on the regulation of the intestinal microbiota have aroused the interest of many scholars. Fecal microbiota transplantation refers to the transplantation of functional flora from the feces of healthy people into the gastrointestinal tract of subjects to repair and reconstruct the intestinal flora. FMT has already shown amazing therapeutic effects in refractory and recurrent clostridium difficile infection， inflammatory bowel disease， irritable bowel syndrome， chronic functional constipation， intestinal cancer and other diseases， but its mechanism of action and safety are not completely clear， and the technology has not been widely used. This paper mainly reviews the definition， mechanism， the role and application prospect of fecal bacteria transplantation technology in gastrointestinal diseases. Reference | Related Articles | Metrics