国际肿瘤学杂志››2015,Vol. 42››Issue (7): 532-534.doi:10.3760/cma.j.issn.1673-422X.2015.07.015
冯飞,秦颂兵
出版日期:
2015-07-08发布日期:
2015-05-26通讯作者:
秦颂兵 E-mail:qin92244@163.comFeng Fei, Qin Songbing
Online:
2015-07-08Published:
2015-05-26Contact:
Qin Songbing E-mail:qin92244@163.com摘要:三维细胞培养是目前公认的能较好模拟体内肿瘤细胞微环境及细胞间相互作用的体外模型。与传统二维细胞培养相比,它在观察肿瘤细胞生物学行为、耐药及放射不敏感、体内基因表达等研究中具有明显优势,能为肿瘤基础实验研究提供极具价值的体外模型。
冯飞,秦颂兵. 三维细胞模型在肿瘤实验中的应用[J]. 国际肿瘤学杂志, 2015, 42(7): 532-534.
Feng Fei, Qin Songbing. Application of threedimensional cell culture model in cancer research[J]. Journal of International Oncology, 2015, 42(7): 532-534.
[1] Tang JH, Cui JF, Chen RX, et al. A threedimensional cell biology model of human hepatocellular carcinoma in vitro[J]. Tumour Biol, 2011, 32(3): 469-479. [2] Tsuji K, Kawauchi S, Saito S, et al. Breast cancer cell lines carry cell linespecific genomic alterations that are distinct from aberrations in breast cancer tissues: comparison of the CGH profiles between cancer cell lines and primary cancer tissues[J]. BMC Cancer, 2010, 10: 15. [3] Perkel JM. Curing cell lines[J]. Biotechniques, 2011, 51(2): 85-90. [4] Cheon DJ, Orsulic S. Mouse models of cancer[J]. Annu Rev Pathol, 2011, 6: 95-119. [5] Herschkowitz JI, Simin K, Weigman VJ, et al. Identification of conserved gene expression features between murine mammary carcinoma models and human breast tumors[J]. Genome Biol, 2007, 8(5): R76. [6] Larue L, Beermann F. Cutaneous melanoma in genetically modified animals[J]. Pigment Cell Res, 2007, 20(6): 485-497. [7] Ghajar CM, Bissell MJ. Tumor engineering: the other face of tissue engineering[J]. Tissue Eng Part A, 2010, 16(7): 2153-2156. [8] SantiagoWalker A, Li L, Haass NK, et al. Melanocytes: from morphology to application[J]. Skin Pharmacol Physiol, 2009, 22(2): 114-121. [9] Pampaloni F, Reynaud EG, Stelzer EH. The third dimension bridges the gap between cell culture and live tissue[J]. Nat Rev Mol Cell Biol, 2007, 8(10): 839-845. [10] Hanahan D, Weinberg RA. Hallmarks of cancer: the next generation[J]. Cell, 2011, 144(5): 646-674. [11] De Wever O, Pauwels P, De Craene B, et al. Molecular and pathological signatures of epithelialmesenchymal transitions at the cancer invasion front[J]. Histochem Cell Biol, 2008, 130(3): 481-494. [12] Lopez JI, Kang I, You WK, et al. In situ force mapping of mammary gland transformation[J]. Integr Biol (Camb), 2011, 3(9): 910-921. [13] Egeblad M, Ewald AJ, Askautrud HA, et al. Visualizing stromal cell dynamics in different tumor microenvironments by spinning disk confocal microscopy[J]. Dis Model Mech, 2008, 1(2-3): 155-167; discussion 165. [14] Holliday DL, Brouilette KT, Markert A, et al. Novel multicellular organotypic models of normal and malignant breast: tools for dissecting the role of the microenvironment in breast cancer progression[J]. Breast Cancer Res, 2009, 11(1): R3. [15] Novitskaya V, Romanska H, Dawoud M, et al. Tetraspanin CD151 regulates growth of mammary epithelial cells in threedimensional extracellular matrix: implication for mammary ductal carcinoma in situ[J]. Cancer Res, 2010, 70(11): 4698-4708. [16] LaBarge MA, Nelson CM, Villadsen R, et al. Human mammary progenitor cell fate decisions are products of interactions with combinatorial microenvironments[J].Integr Biol (Camb), 2009, 1(1): 70-79. [17] Zhou S, Li F, Xiao J, et al. Isolation and identification of cancer stem cells from human osteosarcom by serumfree threedimensional culture combined with anticancer drugs[J]. J Huazhong Univ Sci Technol Med Sci, 2010, 30(1): 81-84. [18] WangZZ, AuP, ChenT, et al. Endothelial cells derivedfromhuman embryonic stem cells form durable blood vessels in vivo[J]. Nat Biotechnol, 2007, 25(3): 317-318. [19] Micalizzi DS, Ford HL. Epithelialmesenchymal transition in development and cancer[J]. Future Oncol, 2009, 5(8): 1129-1143. [20] Crigler L, Kazhanie A, Yoon TJ, et al. Isolation of a mesenchymal cell population from murine dermis that contains progenitors of multiple cell lineages[J]. FASEB J, 2007, 21(9): 2050-2063. [21] Fischbach C, Chen R, Matsumoto T, et al. Engineering tumors with 3D scaffolds[J]. Nat Methods, 2007, 4(10): 855-860. [22] Nelson CM, Inman JL, Bissell MJ. Threedimensional lithographically defined organotypic tissue arrays for quantitative analysis of morphogenesis and neoplastic progression[J]. Nat Protoc, 2008, 3(4): 674-678. [23] Nelson CM, Khauv D, Bissell MJ, et al. Change in cell shape is required for matrix metalloproteinaseinduced epithelialmesenchymal transition of mammary epithelial cells[J]. J Cell Biochem, 2008, 105(1): 25-33. [24] Jo M, Eastman BM, Webb DL, et al. Cell signaling by urokinasetype plasminogen activator receptor induces stem celllike properties in breast cancer cells[J]. Cancer Res, 2010, 70(21): 8948-8958. [25] Indovina P, Rainaldi G, Santini MT. Hypoxia increases adhesion and spreading of MG63 threedimensional tumor spheroids[J]. Anticancer Res, 2008, 28(2A): 1013-1022. [26] Harma V, Virtanen J, Makela R, et al. A comprehensive panel of threedimensional models for studies of prostate cancer growth, invasion and drug responses[J]. PLoS One, 2010, 5(5): e10431. |
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