Abstract:ObjectiveTo observe the effects of oldhamianoside Ⅱ on the invasive ability of prostatic carcinoma DU-145 cells, and to investigate the potential mechanism. MethodsAfter treatment of DU-145 cells with oldhamianoside Ⅱ, MTT assay was used to test the effect of oldhamianoside Ⅱ on the cell proliferation.The invasive ability was assessed with a transwell cell culture chamber. Immunocytochemistry stain was used to investigate the expression of matrix metalloproteinase (MMP-2) and CXCchemokine receptor 4 (CXCR4). ResultsThe proliferation of DU-145 cells was inhibited after treatment with oldhamianoside Ⅱ. In vitro invasion assay indicated that the cells moved through the membrane were (180.3±14.6)／field in the control group, while decreased to (100.4±1.5), (80.2±2.7) and (60.1±5.3)／field in 0.25, 0.5 and 1 μg/mL oldhamianoside Ⅱ treated DU145 cells respectively. Comparing to control group, the cells of oldhamianoside Ⅱ groups moved through the membrane were decreased remarkably (P＜0.05). Immunocytochemistry showed that the expression of MMP-2 and CXCR4 were decreased of oldhamianoside Ⅱ groups. ConclusionsOldhamianoside Ⅱ can inhibit the invasiveness of DU-145 cells, which is related to the down regulation the expression of MMP-2 in DU-145 cells. Oldhamianoside Ⅱ can inhibit the prostate cancer cells metastasis to the bone, which is related to the down regulation the expression of CXCR4 in DU-145 cells.

Key words:Prostatic neoplasms,Matrix metalloproteinases,Receptors, CXCR4,Oldhamianoside Ⅱ