Abstract:ObjectiveTo explore the expressions and clinical significances of deleted in liver cancer1 (DLC1) and Rho associated coiledcoil forming protein kinase (ROCK)Ⅰ in nonsmall lung cancer (NSCLC). MethodsThe expressions of DLC1 and ROCKⅠ in NSCLC and adjacent tissue of 48 patients with pathologically confirmed as NSCLC and undergone surgical resection were detected by immunohistochemistry EnVision method. The correlations among DLC1 protein, ROCKⅠ protein and the clinical pathological characteristics were analyzed. The prognostic value of DLC1 in patients with NSCLC was studied. ResultsThe expression of DLC1 protein in NSCLC tissue was low or missing, and the positive rate was 33.3% (16/48), significantly lower than that in the tissue adjacent to carcinoma 70.8% (34/48), with statistical significance (χ2=13.523, P<0.01). The positive expression rate of ROCKⅠ protein in NSCLC was 58.3% (28/48), higher than that of tissue adjacent to carcinoma 0（0/48）, with statistical significance (χ2=39.529, P<0.01). The expression of DLC1 protein was correlated with tumor differentiation, lymph node metastasis and clinical stage, rather than with sex, smoking history and organization type. Through the correlation analysis, the expression of ROCKⅠ in DLC1 positive group was 37.5% (6/16), and the expression rate of ROCKⅠ in DLC1 negative group was 68.8% (22/32). There was negative correlation between DLC1 and ROCKⅠ in NSCLC tissues (r=-2.214, P=0.039). The 3 year survival rate in DLC1 protein high expression group was obviously higher than that in low expression group, with statistical significance (P=0.043). ConclusionLow or missing expression of DLC1 and high expression of ROCKⅠ protein may play an important role in the occurrence and development of NSCLC. Detecting the expression of DLC1 and ROCKⅠ protein may be useful for evaluating the biological behavior and prognosis of NSCLC.

Key words:Carcinoma, nonsmallcell lung,Prognosis,DLC-1,ROCKⅠ