1例云南汉族大肠癌中NDRG1全长基因扩增及特征分析

betway必威登陆网址（betway.com ）学报››2023,Vol. 44››Issue (4): 268-272.DOI:10.3969/j.issn.2097-0005.2023.04.006

1例云南汉族大肠癌中NDRG1全长基因扩增及特征分析

杜娟¹^,²(), 沈红³(), 王芳², 廖文平⁴(), 王开昕⁵()

^1.合肥市第一人民医院病理科，安徽合肥 230601
^2.昆明医科大学基础医学院病理学与病理生理学系，云南昆明 650500
^3.昭通市第一人民医院病理科，云南昭通 657000
^4.云南省药物研究所安全性评价中心毒性病理研究室，云南昆明 650111
^5.华中科技大学协和深圳医院病理科，广东深圳 518052

收稿日期:2022-10-28出版日期:2023-04-25发布日期:2023-05-15
通讯作者:廖文平,王开昕
作者简介:杜娟，硕士，住院医师，研究方向：肿瘤病理，E-mail：504278653@qq.com
沈红，硕士，副主任医师，研究方向：肿瘤病理，E-mail：568178306@qq.com第一联系人：杜娟、沈红为共同第一作者。
基金资助:
国家自然科学基金(81260361)

Amplification and characterization of full-length NDRG1 gene in a case of colorectal cancer of Yunnan Han nationality

Juan DU¹^,²(), Hong SHEN³(), Fang WANG², Wenping LIAO⁴(), Kaixin WANG⁵()

^1.Department of Pathology，the First People's Hospital of Hefei，Hefei 230001，China
^2.Department of Pathology and Pathophysiology，School of Basic Medicine，Kunming Medical University，Kunming 650500，China
^3.Department of Pathology，the First People's Hospital of Zhaotong，Kunming 657000，China
^4.Laboratory of toxicity Pathology，Drag safety evaluation center of Yunnan institute of Materia Medica，Kunming 650111，China
^5.Department of Pathology，Huazhong University of Science and Technology Union Shenzhen Hospital，Shenzhen 518052，China

Received:2022-10-28Online:2023-04-25Published:2023-05-15
Contact:Wenping LIAO,Kaixin WANG

摘要/Abstract

摘要：

目的分析云南汉族大肠癌与高加索人大肠癌细胞中N?myc下游调节基因（N?myc down?stream regulated gene 1， NDRG1）的差异。方法收集云南汉族大肠癌患者的腹水，进行癌细胞培养及传代，同时选择高加索人大肠癌细胞系Caco2进行培养。通过实时荧光定量PCR，在mRNA水平观察NDRG1在云南汉族及高加索人大肠癌细胞中的表达特点。结果云南汉族与高加索人大肠癌细胞NDRG1全长序列一致，云南汉族大肠癌中NDRG1 mRNA的表达明显高于高加索人。结论NDRG1基因在云南汉族及高加索人大肠癌细胞中核酸序列相同，但mRNA表达水平存在一定差异。

关键词:NDRG1,云南,汉族,大肠癌

Abstract:

ObjectiveTo explore the difference of NDRG1 gene between the Han nationality in Yunnan and Caucasian colorectal cancer cells.MethodsAscites from Yunnan Han nationality patients with colorectal cancer were collected for cancer cell culture and passage， and Caucasian colorectal cancer cell line Caco2 was selected for culture. The expression characteristics of NDRG1 in Yunnan Han nationality and Caucasian colorectal cancer cells were observed at mRNA level by real-time fluorescence quantitative PCR.ResultsThe full-length sequence of NDRG1 in a case of Yunnan Han nationality and Caucasian colorectal cancer cells was consistent, the expression of NDRG1 mRNA in colorectal cancer of Yunnan Han nationality was significantly higher than that of Caucasian.ConclusionIt is speculated that the nucleic acid sequence of NDRG1 gene are the same in Yunnan Han nationality and Caucasian colorectal cancer cells， but the mRNA expression level is different.

Key words:NDRG1,Yunnan,Han nationality,colorectal carcinoma

杜娟, 沈红, 王芳, 廖文平, 王开昕. 1例云南汉族大肠癌中NDRG1全长基因扩增及特征分析[J]. betway必威登陆网址（betway.com ）学报, 2023, 44(4): 268-272.

Juan DU, Hong SHEN, Fang WANG, Wenping LIAO, Kaixin WANG. Amplification and characterization of full-length NDRG1 gene in a case of colorectal cancer of Yunnan Han nationality[J]. Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences, 2023, 44(4): 268-272.

图/表6

表1用于实时荧光定量聚合酶链式反应的引物

基因	引物编号	引物序列	退火温度/℃	产物长度/bp
NDRG1-1	NDRG1-1-S	5'-TCGAGACTTTACATGGCTCTGTTC-3'	52	1 185
NDRG1-1	NDRG1-1-A	5'-GGTTGTAGCAGGTTTTGTGGTTC-3'
NDRG1-2	NDRG1-1-S	5'-GAAGAAATGCAGAGTAACGTGGAA-3'
NDRG1-2	NDRG1-1-A	5'-TCCCCAACCACCAACAGAG-3'
GAPDH	GAPDH-S	5'-GCACCGTCAAGGCTGAGAAC-3'	55	380
GAPDH	GAPDH-A	5'-TGGTGAAGACGCCAGTGGA-3'

图1云南汉族大肠癌细胞培养第1天（× 200）

图2云南汉族大肠癌细胞传代第1代（× 200）

图3云南汉族大肠癌细胞NDRG1扩增图谱

图4NDRG1基因连接PMD19-T载体转化入大肠杆菌DH5a感受态细胞单克隆摇菌后菌液质粒提取图谱

图5HindⅢ及BamHI双酶切NDRG1基因及PMD19-T载体的酶切图谱

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