国际肿瘤学杂志

国际肿瘤学杂志››2012,Vol. 39››Issue (6): 477-480.

• 论著 •上一篇

MALAT1、COX-2、β-catenin、MMP-3、MMP-9等基因在结直肠癌发生发展中的意义

季青,周宁,刘宣,殷佩浩,秦建民,李琦

  1. 上海中医药大学附属普陀医院 上海中医药大学中西医结合肿瘤介入研究所
  • 出版日期:2012-06-08发布日期:2012-06-25
  • 通讯作者:李琦, E-mail: lzwf@hotmail.com E-mail:lzwf@hotmail.com
  • 基金资助:

    上海市科学技术委员会基金资助项目(10ZR1427400、10140902600、11411963100); 上海市重点学科基金资助项目(S30302); 上海市科学技术委员会基金资助项目(2010JW62)；上海市卫生局青年科研基金资助项目（20114037、20114y001）

Significance of MALAT1，COX-2,β-catenin,MMP-3 and MMP-9 in the occurrence and development of colorectal carcinoma

JI Qing, ZHOU Ning, LIU Xuan, YIN Pei-hao, QIN Jian-ming, LI Qi

  1. PU Tuo Hospital, Shang Hai University of Traditional Chinese Medicine, Interventional Cancer Institute of Chinese Integrative Medicine, Shang Hai University of Traditional Chinese Medicine, Shanghai 20062, China
  • Online:2012-06-08Published:2012-06-25
  • Contact:LI Qi, E-mail: lzwf@hotmail.com E-mail:lzwf@hotmail.com

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摘要：目的 探讨人肺腺癌转移相关转录因子1（MALAT1）、环氧合酶-2（COX-2）、β-连环素（β-catenin）、基质金属蛋白酶（MMP）-3、MMP-9等基因在结直肠癌发生发展中的意义。 方法 收集新鲜结直肠癌组织标本及其对应癌旁组织标本各30例，采用实时荧光定量聚合酶链反应（PCR）方法检测MALAT1、COX-2、β-catenin、MMP-3、MMP-9的mRNA表达水平，并与患者性别、年龄、癌胚抗原(CEA)、细胞免疫因子（CD4和CD8）、临床分期、肿瘤侵袭转移程度等进行相关性分析。 结果 MALAT1、COX-2、β-catenin、MMP-9在癌组织和癌旁组织中的表达差异均有统计学意义（P<0.05），MMP-3则无统计学意义（P>0.05）。MALAT1、COX-2、MMP-9和β-catenin在癌组织中的表达量分别是癌旁组织表达量的2.22倍、1.86倍、2.16倍、0.58倍 (P<0.01)。MALAT1（癌/癌旁）的表达差异与β-catenin（癌/癌旁）的表达差异呈负相关（r＝-0.346，P=0.030），MMP-9（癌/癌旁）的表达差异与β-catenin（癌/癌旁）的表达差异则呈正相关(r=0.312，P=0.047)。COX-2（癌/癌旁）与MMP-9（癌/癌旁）在男女患者之间的差异均有统计学意义（P=0.047；P=0.018）；COX-2（癌/癌旁）在CEA上升与未见改变的患者之间的差异也有统计学意义（P=0.021）。结论 MALAT1、COX-2、MMP-9、β-catenin在结直肠癌的发生发展中有重要意义，而MMP-3无重要参考价值；MALAT1和β-catenin的负相关性及MMP-9和β-catenin的正相关性说明其在结直肠癌的发生发展中存在一定的相互作用关系；COX-2（癌/癌旁）和MMP-9（癌/癌旁）在男女患者中的差异提示二者影响男女患结直肠癌的概率；检测COX-2可在一定程度上辅助肿瘤标志物CEA对结直肠癌的诊断。

关键词:结直肠肿瘤,环氧化酶2,β连环素,基质金属蛋白酶3,基质金属蛋白酶9,人肺腺癌转移相关转录因子1

Abstract:Objective To investigate the significance of metastasis associated lung adenocarcinoma transcript 1 (MALAT1),cyclooxygenase-2 (COX-2),beta catenin (β-catenin)、matrix metalloproteinase (MMP)-3 and MMP-9 in the occurrence and development of colorectal carcinoma. Methods Real-time PCR was used to detect MALAT1,COX-2,β-catenin,MMP-3 and MMP-9 mRNA expression in samples from 30 fresh colorectal carcinomas and 30 corresponding adjacent tissues. And the correlation analysis of the gender and age of patients, CEA, immune cellular factors (CD4 and CD8), clinical stages, and the degree of differentiation was undertaken. Results The expression levels of MALAT1,COX-2,β-catenin and MMP-9 were significantly different between colorectal carcinoma tissues and adjacent colorectal tissues (P<0.05). MMP-3 showed no significant difference (P>0.05). MALAT1,COX-2,β-catenin and MMP-9 expression levels showed an average 2.22fold, 1.86fold, 2.16fold, 0.58fold (P<0.01) increase in colorectal carcinoma tissues when compared with adjacent colorectal tissues respectively. There were negative correlation between MALAT1 and β-catenin (colorectal carcinoma tissues vs adjacent colorectal tissues) (r=-0.346, P=0.030). While there were positive correlation between MMP-9 and β-catenin (colorectal carcinoma tissues vs adjacent colorectal tissues) (r=0.312, P=0.047). There were significant difference between male patients and female patients in terms of COX-2 and MMP-9 (colorectal carcinoma tissues vs adjacent colorectal tissues) (P= 0.047; P=0.018). There were significant difference between patients with tumor marker CEA increase and patients without CEA increase in terms of COX-2 (colorectal carcinoma tissues vs adjacent colorectal tissues) (P=0.021). Conclusion MALAT1,COX-2,MMP-9 and β-catenin have significance in the occurrence and development of colorectal carcinoma, while MMP-3 has no significant reference value. The negative correlation between MALAT1 and β-catenin and the positive correlation between MMP-9 and β-catenin might show some interaction relationship in the development of colorectal carcinoma. The expression differences of COX-2 and MMP-9 (colorectal carcinoma tissues vs adjacent colorectal tissues) in male and female patients suggest that above two genes may affect the occurrence ratio of colorectal carcinoma. Detecting of COX-2 maybe helpful to the tumor marker CEA during the diagnosis of colorectal carcinoma.

Key words:Colorectal neoplasms,Cyclooxygenase 2,beta Catenin,Matrix metalloproteinase 3,Matrix metalloproteinas 9,Metastasis associated lung adenocarcinoma transcript 1