Numb inhibits proliferation and migration of human bladder cancer cell line BIU87 by negative modulating CXCR4

Abstract

Abstract:ObjectiveTo study the effect of Numb gene on CXCR4 expression and proliferation and migration of bladder cancer. MethodsHuman bladder cancer cells were divided into 3 groups: experimental group (transfected with NumbORF plasmid), negativecontrol group (transfected with blank vector), and blankcontrol group (no DNA transfected in). The expressions of Numb and CXCR4 were detected by realtime PCR and Western blotting respectively. Cell proliferation and migration were measured by MTS and Transwell assay respectively. ResultsNumb expressions in experimental group, negativecontrol group and blankcontrol group were 31.044±3.350, 4.401±0.567 and 4.287±0.341 respectively, with a statistical significance (F=183.418, P=0.000). CXCR4 levels in experimental group, negativecontrol group and blankcontrol group were 0.344±0.167, 0.996±0.148 and 1.010±0.106 respectively, with a statistical significance (F=21.355, P=0.002). In MTS assay, the absorb values in experimental group, negativecontrol group and blankcontrol group were 0.615±0.057, 0.987±0.063 and 0.957±0.066, with a statistical difference (F=33.210, P=0.001). In Transwell assay, the numbers of migratory cells in experimental group, negativecontrol group and blankcontrol group were 164.667±19.858, 670.133±38.760 and 667.533±27.610, with a statistical significance (F=286.788, P=0.000). ConclusionOverexpression of Numb can suppress the ability of proliferation and migration of the BIU87 cells through downregulation of CXCR4 expression in human bladder cancer.

Key words:Urinary bladder neoplasms,Receptors, CXCR4,Cell proliferation,Cell migration assays

Sima Jin, Zhang Bao, Wang Baojun, Yu Yuanzi, Ai Qing, Zhang Xu. Numb inhibits proliferation and migration of human bladder cancer cell line BIU87 by negative modulating CXCR4[J]. Journal of International Oncology, 2015, 42(7): 492-495.

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