关键词:免疫组化,阳性对照,阴性对照,组织微阵列,蜡块,切片

Abstract:Objective: To construct an economic and practical tissue microarray control system, and to introduce a simple method of making tissue microarray wax blocks and sections. Methods: According to the actual clinical work experience, the tissue microarray control system was constructed on the basis of the recommendations of the international special expert committee and the consensus of immunohistochemistry detection technology; The remaining tissue after clinical diagnosis was satisfied in the external examination of clinical pathology, the conventional dehydration treatment was used, the target area was selected under the microscope, the tissue target area was cut into about 0.2 cm×0.2 cm×0.2 cm group woven blocks with waste disposable pathological blade. According to the tissue microarray control system, the target tissue was embedded with a smaller embedding abrasive (about 0.7 cm×0.7 cm), and the tissue microarray wax block was made Tag number; Tissue microarray wax was frozen and sliced. The thickness of the slice was 2～2.5 μm. the wax slice was pasted in the center of 0.5cm from the bottom of the special adhesive slide for immunohistochemistry. The number of positive control tissue microarray was marked with pencil. The slice was baked at 72℃ for 15～30 min or cooled at room temperature to dry, and the tissue microarray slice was made. Results: Five tissue microarray control systems, five tissue microarray wax blocks and five tissue microarray sections were established. Conclusion: Five tissue microarray control systems are designed scientifically and reasonably, which can meet the negative and positive control of more than 100 kinds of antibodies. The production method of tissue microarray wax block is simple, without special instruments and equipment, and the tissue source is sufficient. The production of tissue microarray slice is simple and easy. The construction of the control system of immunohistochemistry tissue microarray is economical and practical. The method of making wax block and slice is simple and easy to operate, which is worth popularizing in clinical work.

Key words:immunohistochemistry,positive control,negative control,tissue microarray,paraffin section