原发性开角型青光眼患者泪液及血清中BDNF的定量分析

摘要/Abstract

摘要： 目的

测定家族性原发性开角型青光眼（primary open angle glaucoma，POAG）及散发性POAG患者泪液、血清脑源性神经营养因子（brain derived neurotrophic factor，BDNF）含量，为寻找POAG早期诊断标记物提供实验基础，并评价其与POAG分期的相关性。

方法

收集符合纳入、排除标准的散发性POAG 患者14例及山东阳谷一POAG遗传家系的6例患者（Ⅲ-8、Ⅲ-10、Ⅲ-12、Ⅲ-18、Ⅲ-20、Ⅲ-31）的临床资料、血液及泪液标本，另外与实验组年龄、性别对应的健康对照者50例，应用人单克隆抗BDNF抗体ELISA试剂盒，按照试剂盒操作步骤对各标本进行BDNF测定。统计学处理应用SPSS17.0统计软件，组间比较采用t检验，不同病程POAG患者BDNF水平比较采用单因素方差分析，两两比较采用LSD-t检验；BDNF水平与年龄相关性分析采用Pearson相关性分析。

结果

显性遗传家系POAG组泪液及血清BDNF较散发POAG组含量减少，但差异无统计学意义（P> 0.05）；POAG患者组泪液BDNF较健康对照组含量减少，差异有统计学意义（P< 0.05）；POAG患者组血清BDNF较健康对照组含量减少，但差异无统计学意义（P> 0.05）。

结论

泪液、血清BDNF在该阳谷家族性POAG致病中的作用有限；血清BDNF受全身多种因素的影响，很难为POAG早期诊断提供有效信息；POAG患者基础泪液的BDNF含量明显减少，患者基础泪液BDNF有望成为POAG早期诊断标记物。

关键词:原发性开角型青光眼,显性遗传家系,神经营养因子,脑源性神经营养因子

Abstract: Objective

To determine the BDNF levels in serum and tears of patients from pedigree and sporadic cases with POAG， which may provide experimental basis for exploring the pathogenesis of POAG and to evaluate its correlation with POAG staging.

Methods

Clinical data， blood and tear samples were collected from 6 patients of one POAG pedigree （Ⅲ-8、Ⅲ-10、Ⅲ-12、Ⅲ-18、Ⅲ-20 and Ⅲ-31） and 14 sporadic cases. 50 age- and sex-matched healthy controls were recruited at the same time. The levels of BDNF were tested by Elisa kit according to its instructions. Statistical analysis was performed using SPSS17.0 statistical software， t test was used for comparison between groups， one-way analysis of variance was used for comparison of BDNF levels in POAG patients with different course of disease， and LSD-t test was used for pair comparison.The correlation between BDNF level and age was analyzed by Pearson correlation analysis.

Results

The serum and tear BDNF levels in POAG pedigree patients were lower than those in sporadic POAG patients. However， the difference was not significant （P> 0.05）； The levels of BDNF in tears of POAG patients were significantly lower than those of normal control group， and the difference was statistically significant （P< 0.05）. Serum BDNF of POAG patients was lower than that of normal control group， and the difference was not significant （P> 0.05）.

Conclusion

The role of serum and tear BDNF in the pathogenesis of POAG pedigree which has pathogenic gene mutations is limited. Serum BDNF is affected by many systemic factors， and it is difficult to provide effective information for the early diagnosis of POAG. The tear BDNF levels in patients with POAG are significantly reduced. The determination of BDNF in basic tears of patients is expected to be a marker for early diagnosis of POAG.

Key words:primary open angle glaucoma,dominant pedigree,neurotrophic factors,brain derived neurotrophic factor

中图分类号:

R775.2

翟玉喜, 冯娜, 高建鲁, 汪鑫. 原发性开角型青光眼患者泪液及血清中BDNF的定量分析[J]. betway必威登陆网址（betway.com ）学报, 2021, 42(7): 516-520.

Yuxi Zhai, Na Feng, Jianlu Gao, Xin Wang. Quantitative analysis of brain-derived neurotrophic factor in serum and tears of primary open angle glaucoma[J]. Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences, 2021, 42(7): 516-520.

图/表9

指标	显性遗传家系POAG患者	散发POAG患者	P
血清BDNF	2.78 ± 2.10	0.63 ± 0.13	0.727
泪液BDNF	3.10 ± 1.72	0.73 ± 0.22	0.299

指标	显性遗传家系POAG患者	散发POAG患者	P
血清BDNF	2.78 ± 2.10	0.63 ± 0.13	0.727
泪液BDNF	3.10 ± 1.72	0.73 ± 0.22	0.299

指标	POAG患者	对照组	P
血清BDNF	3.01 ± 1.79	3.59 ± 3.46	0.510
泪液BDNF	0.70 ± 0.20	0.89 ± 0.32	0.032

指标	POAG患者	对照组	P
血清BDNF	3.01 ± 1.79	3.59 ± 3.46	0.510
泪液BDNF	0.70 ± 0.20	0.89 ± 0.32	0.032

视野缺损	例数	泪液BDNF	F	P
早期	8	0.66 ± 0.13
进展期	4	0.60 ± 0.07	1.68	0.22
晚期	8	0.80 ± 0.26

视野缺损	例数	泪液BDNF	F	P
早期	8	0.66 ± 0.13
进展期	4	0.60 ± 0.07	1.68	0.22
晚期	8	0.80 ± 0.26

视野缺损	例数	血清BDNF	F	P
早期	8	2.82 ± 1.97
进展期	4	3.60 ± 2.22	0.25	0.78
晚期	8	2.90 ± 1.58

视野缺损	例数	血清BDNF	F	P
早期	8	2.82 ± 1.97
进展期	4	3.60 ± 2.22	0.25	0.78
晚期	8	2.90 ± 1.58

血清BDNF	年龄
POAG患者	0.105	0.661
健康对照组	0.304	0.192

血清BDNF	年龄
POAG患者	0.105	0.661
健康对照组	0.304	0.192

泪液BDNF	年龄
POAG患者	0.061	0.800
健康对照组	0.537	0.015

泪液BDNF	年龄
POAG患者	0.061	0.800
健康对照组	0.537	0.015

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