吸入高浓度H<sub>2</sub>对高脂饮食大鼠血浆和肝脏中磷脂和鞘脂分子的影响

摘要/Abstract

摘要： 目的

H₂吸入对脂质代谢有一定的调节作用，但对磷脂、鞘脂分子的具体影响尚无研究。本研究通过靶向脂质组学方法检测了66.7%的H₂吸入干预后高脂饮食大鼠血浆及肝脏中的磷脂、鞘脂分子的变化，为研究H₂改善脂代谢的机制研究提供依据。

方法

选取雄性SD大鼠分为对照组、高脂饮食模型组，高脂饮食 + 66.7% H₂干预组。干预10周后，对血浆和肝脏中的不同种类的磷脂和鞘脂进行分析。

结果

主成分分析及聚类热图表明高脂饮食模型组与对照组之间的磷脂、鞘脂谱存在明显差异，在血浆中检测出差异磷脂分子17种，差异鞘脂分子16种；在肝脏中检测出差异磷脂分子50种，鞘脂分子18种。66.7% H₂干预后对血浆的磷脂和鞘脂均有影响，共计5种磷脂酰胆碱、2种溶血磷脂酰乙醇胺、1种鞘磷脂分子含量降低，差异有统计学意义（P< 0.05）；66.7% H₂干预后对肝脏磷脂影响较大，共12种磷脂酰胆碱、6种磷脂酰乙醇胺、3种溶血磷脂酰胆碱、4种溶血磷脂酰乙醇胺分子含量降低，差异有统计学意义（P< 0.05），而对神经酰胺、鞘磷脂等鞘脂无显著影响。

结论

66.7%的H₂吸入可影响高脂饮食大鼠磷脂、鞘脂代谢，降低血浆及肝脏中多种磷脂的含量，改善高脂饮食导致的血浆、肝脏中多种磷脂、鞘脂分子水平的异常。H₂可作为一种改善脂质代谢的有效辅助手段。H₂改善脂质代谢的分子机制值得进一步研究。

关键词:氢气,高脂饮食,磷脂,鞘脂

Abstract: Objective

Hydrogen inhalation has a certain regulatory effect on lipid metabolism， but the specific effects on phospholipids and sphingolipids are not yet studied. In this article， the changes of phospholipids and sphingolipids were detected by targeted lipidomics method after inhalation of 66.7% hydrogen gas.

Methods

Male SD rats were divided into control group， high-fat diet model group and high-fat diet + 66.7% H₂intervention group. After 10 weeks of intervention， different kinds of phospholipids and sphingolipids in plasma and liver of the rats were analyzed.

Results

Targeted lipidomics analysis showed differential hepatic and plasmatic effects of control and modern group， including 17 differential phospholipids and 16 differential sphingolipids in plasma， and 50 differential phospholipids and 18 differential sphingolipids liver. Phospholipids and sphingolipids in plasma and liver were significantly changed after 66.7% hydrogen intervention， including 5 phosphatidylcholines， 2 lysophosphatidylethanolamines and 1 sphingomyelin decreased significantly in plasma； 12 phosphatidylcholines， 6 phosphatidylethanolamines， 3 lysophosphatidylcholines， and 4 lysophosphatidyl ethanolamines decreased in liver， but there was no significant effects on ceramides and sphingomyelin sphingolipids.

Conclusion

66.7% hydrogen inhalation can affect the metabolism of phospholipids and sphingolipids， and reduce the content of phospholipids， improve the abnormal levels of phospholipids and sphingolipids in plasma and liver of high-fat diet rats. Hydrogen may be an auxiliary means to improve lipid metabolism. The molecular mechanism of hydrogen improving lipid metabolism deserves further study.

Key words:hydrogen,high-fat diet,phospholipids,sphingolipids

谢云博, 刘伯言, 陈军, 张驰, 赵敏, 秦树存, 王义围. 吸入高浓度H₂对高脂饮食大鼠血浆和肝脏中磷脂和鞘脂分子的影响[J]. betway必威登陆网址（betway.com ）学报, 2022, 43(4): 241-247.

Yunbo XIE, Boyan LIU, Jun Chen, Chi ZHANG, min ZHAO, Shuncun QIN, Yiwei WANG. Effects of high concentration of H₂inhalation on phospholipids and sphingolipids in plasma and liver of high-fat diet rats[J]. Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences, 2022, 43(4): 241-247.

图/表5

Compounds	Q1（Parent ion）	Q3（Product ion）^a	DP（V）	CE（V）
PC	［M + H］⁺	PI 184.1	100	40
PE	［M + H］⁺	NL 141	100	30
LPC	［M + H］⁺	PI 184.1	110	40
LPE	［M + H］⁺	NL 141	100	25
Cer	［M + H］⁺	PI 264.3	80	40
SM	［M + H］⁺	PI 184.1	110	40

Compounds	Q1（Parent ion）	Q3（Product ion）^a	DP（V）	CE（V）
PC	［M + H］⁺	PI 184.1	100	40
PE	［M + H］⁺	NL 141	100	30
LPC	［M + H］⁺	PI 184.1	110	40
LPE	［M + H］⁺	NL 141	100	25
Cer	［M + H］⁺	PI 264.3	80	40
SM	［M + H］⁺	PI 184.1	110	40

脂质分子	relative contents （ratio to IS）
PC 16∶0/18∶0	0.099 ± 0.017	0.124 ± 0.052	0.086 ± 0.026^#
PC 16∶1/18∶1	0.026 ± 0.007	0.052 ± 0.008^***	0.039 ± 0.011^#
PC 18∶2/18∶2	0.394 ± 0.083	0.306 ± 0.082^*	0.216 ± 0.075^#
PC 18∶1/18∶2	0.538 ± 0.158	0.524 ± 0.136	0.393 ± 0.109^#
PC 18∶1/18∶1	0.149 ± 0.035	0.284 ± 0.087^***	0.196 ± 0.051^##
LPE 18∶1	0.326 ± 0.068	0.457 ± 0.190^*	0.291 ± 0.079^##
LPE 20∶4	0.095 ± 0.030	0.114 ± 0.035	0.077 ± 0.200^##
SM d18∶1/18∶2	0.489 ± 0.115	0.205 ± 0.250^***	0.157 ± 0.024^###

脂质分子	relative contents （ratio to IS）
PC 16∶0/18∶0	0.099 ± 0.017	0.124 ± 0.052	0.086 ± 0.026^#
PC 16∶1/18∶1	0.026 ± 0.007	0.052 ± 0.008^***	0.039 ± 0.011^#
PC 18∶2/18∶2	0.394 ± 0.083	0.306 ± 0.082^*	0.216 ± 0.075^#
PC 18∶1/18∶2	0.538 ± 0.158	0.524 ± 0.136	0.393 ± 0.109^#
PC 18∶1/18∶1	0.149 ± 0.035	0.284 ± 0.087^***	0.196 ± 0.051^##
LPE 18∶1	0.326 ± 0.068	0.457 ± 0.190^*	0.291 ± 0.079^##
LPE 20∶4	0.095 ± 0.030	0.114 ± 0.035	0.077 ± 0.200^##
SM d18∶1/18∶2	0.489 ± 0.115	0.205 ± 0.250^***	0.157 ± 0.024^###

脂质分子	relative contents （ratio to IS/mg protein）
PC 16∶0/18∶2	8.551 ± 2.077	25.60 ± 7.130^***	16.61 ± 5.260^##
PC 16∶0/18∶0	0.681 ± 50.179	1.95 ± 0.607^***	1.40 ± 0.410^#
PC 18∶0/18∶0	0.246 ± 0.046	0.79 ± 0.190^***	0.55 ± 0.200^#
PC 16∶0/20∶2	0.019 ± 0.005	0.058 ± 0.015^***	0.042 ± 0.014^#
PC 16∶1/18∶1	0.0218 ± 0.009	0.196 ± 0.078^***	0.102 ± 0.044^##
PC 16∶0/18∶1	1.066 ± 0.244	6.295 ± 1.640^***	3.921 ± 1.140^##
PC 16∶0/20∶5	0.040 ± 0.010	0.123 ± 0.056^**	0.072 ± 0.028^#
PC 16∶0/20∶4	4.396 ± 0.983	11.050 ± 2.529^***	8.060 ± 2.598^#
PC 18∶1/18∶1	0.121 ± 0.040	0.789 ± 0.214^***	0.470 ± 0.163^##
PC 18∶0/18∶1	0.792 ± 0.232	3.767 ± 1.540^***	2.713 ± 0.644^#
PC 16∶0/22∶6	0.174 ± 0.499	0.310 ± 0.055^***	0.231 ± 0.0796^#
PC 18∶1/20∶4	0.283 ± 0.099	0.850 ± 0.238^***	0.5782 ± 0.186^#
PE 18∶0/18∶2	9.716 ± 3.464	21.732 ± 7.483^**	14.642 ± 2.682^#
PE 18∶2/22∶5	0.067 ± 0.024	0.183 ± 0.062^***	0.121 ± 0.036^#
PE 18∶0/22∶5	1.500 ± 0.470	4.067 ± 0.899^***	3.167 ± 0.770^#
PE 18∶0/22∶4	0.542 ± 0.131	1.323 ± 0.426^***	0.869 ± 0.207^##
PE 20∶0/20∶4	0.192 ± 0.056	0.451 ± 0.112^***	0.360 ± 0.077^#
PE 18∶0/20∶6	6.755 ± 1.954	17.610 ± 3.081^***	13.279 ± 3.866^#
LPC 18∶0	16.320 ± 4.198	50.110 ± 11.720^***	36.271 ± 9.397^##
LPC 20∶1	1.695 ± 4.499	0.332 ± 0.097	0.202 ± 0.077^##
LPC 22∶6	0.157 ± 0.076	0.211 ± 0.066	0.159 ± 0.026^#
LPE 16∶0	2.522 ± 1.228	8.501 ± 3.287^***	6.089 ± 1.109^#
LPE 18∶0	6.639 ± 1.916	23.375 ± 6.631^***	14.150 ± 3.061^###
LPE 18∶1	0.716 ± 0.237	2.903 ± 1.204^***	1.792 ± 0.483^##
LPE 20∶1	0.019 ± 0.008	0.060 ± 0.018^***	0.035 ± 0.009^###

脂质分子	relative contents （ratio to IS/mg protein）
PC 16∶0/18∶2	8.551 ± 2.077	25.60 ± 7.130^***	16.61 ± 5.260^##
PC 16∶0/18∶0	0.681 ± 50.179	1.95 ± 0.607^***	1.40 ± 0.410^#
PC 18∶0/18∶0	0.246 ± 0.046	0.79 ± 0.190^***	0.55 ± 0.200^#
PC 16∶0/20∶2	0.019 ± 0.005	0.058 ± 0.015^***	0.042 ± 0.014^#
PC 16∶1/18∶1	0.0218 ± 0.009	0.196 ± 0.078^***	0.102 ± 0.044^##
PC 16∶0/18∶1	1.066 ± 0.244	6.295 ± 1.640^***	3.921 ± 1.140^##
PC 16∶0/20∶5	0.040 ± 0.010	0.123 ± 0.056^**	0.072 ± 0.028^#
PC 16∶0/20∶4	4.396 ± 0.983	11.050 ± 2.529^***	8.060 ± 2.598^#
PC 18∶1/18∶1	0.121 ± 0.040	0.789 ± 0.214^***	0.470 ± 0.163^##
PC 18∶0/18∶1	0.792 ± 0.232	3.767 ± 1.540^***	2.713 ± 0.644^#
PC 16∶0/22∶6	0.174 ± 0.499	0.310 ± 0.055^***	0.231 ± 0.0796^#
PC 18∶1/20∶4	0.283 ± 0.099	0.850 ± 0.238^***	0.5782 ± 0.186^#
PE 18∶0/18∶2	9.716 ± 3.464	21.732 ± 7.483^**	14.642 ± 2.682^#
PE 18∶2/22∶5	0.067 ± 0.024	0.183 ± 0.062^***	0.121 ± 0.036^#
PE 18∶0/22∶5	1.500 ± 0.470	4.067 ± 0.899^***	3.167 ± 0.770^#
PE 18∶0/22∶4	0.542 ± 0.131	1.323 ± 0.426^***	0.869 ± 0.207^##
PE 20∶0/20∶4	0.192 ± 0.056	0.451 ± 0.112^***	0.360 ± 0.077^#
PE 18∶0/20∶6	6.755 ± 1.954	17.610 ± 3.081^***	13.279 ± 3.866^#
LPC 18∶0	16.320 ± 4.198	50.110 ± 11.720^***	36.271 ± 9.397^##
LPC 20∶1	1.695 ± 4.499	0.332 ± 0.097	0.202 ± 0.077^##
LPC 22∶6	0.157 ± 0.076	0.211 ± 0.066	0.159 ± 0.026^#
LPE 16∶0	2.522 ± 1.228	8.501 ± 3.287^***	6.089 ± 1.109^#
LPE 18∶0	6.639 ± 1.916	23.375 ± 6.631^***	14.150 ± 3.061^###
LPE 18∶1	0.716 ± 0.237	2.903 ± 1.204^***	1.792 ± 0.483^##
LPE 20∶1	0.019 ± 0.008	0.060 ± 0.018^***	0.035 ± 0.009^###

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吸入高浓度H₂对高脂饮食大鼠血浆和肝脏中磷脂和鞘脂分子的影响

Effects of high concentration of H₂inhalation on phospholipids and sphingolipids in plasma and liver of high-fat diet rats

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脂质分子	relative contents （ratio to IS）
脂质分子	Con	HFD	HFD+H₂
PC 16∶0/18∶0	0.099 ± 0.017	0.124 ± 0.052	0.086 ± 0.026^#
PC 16∶1/18∶1	0.026 ± 0.007	0.052 ± 0.008^***	0.039 ± 0.011^#
PC 18∶2/18∶2	0.394 ± 0.083	0.306 ± 0.082^*	0.216 ± 0.075^#
PC 18∶1/18∶2	0.538 ± 0.158	0.524 ± 0.136	0.393 ± 0.109^#
PC 18∶1/18∶1	0.149 ± 0.035	0.284 ± 0.087^***	0.196 ± 0.051^##
LPE 18∶1	0.326 ± 0.068	0.457 ± 0.190^*	0.291 ± 0.079^##
LPE 20∶4	0.095 ± 0.030	0.114 ± 0.035	0.077 ± 0.200^##
SM d18∶1/18∶2	0.489 ± 0.115	0.205 ± 0.250^***	0.157 ± 0.024^###

吸入高浓度H2对高脂饮食大鼠血浆和肝脏中磷脂和鞘脂分子的影响

Effects of high concentration of H2inhalation on phospholipids and sphingolipids in plasma and liver of high-fat diet rats

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吸入高浓度H₂对高脂饮食大鼠血浆和肝脏中磷脂和鞘脂分子的影响

Effects of high concentration of H₂inhalation on phospholipids and sphingolipids in plasma and liver of high-fat diet rats