国际肿瘤学杂志

国际肿瘤学杂志››2012,Vol. 39››Issue (1): 64-67.

• 论著 •上一篇下一篇

人脑胶质瘤EGFL7 表达与FAKpY397、MVD相关性研究

薛伟明, 王占祥, 马永会, 等

  1. 361003厦门大学第一附属医院神经外科
  • 出版日期:2012-01-08发布日期:2013-11-11
  • 通讯作者:薛伟明， E-mail: 13306006556@sohu.com E-mail:13306006556@sohu.com
  • 基金资助:

    厦门市卫生局青年创新科研项目WQK0514

Expression of EGFL7 in human glioma and its relationship with FAKpY397 and MVD

XUE Wei-Ming, WANG Zhan-Xiang, MA Yong-Hui, et al

  1. Department ofNeurosurgery,First Affiliated Hospital of Xiamen University , Xiamen 361000, China

  • Online:2012-01-08Published:2013-11-11
  • Contact:Corresponding author: XUE Wei-ming, E-mail: 13306006556@sohu.com E-mail:13306006556@sohu.com

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摘要：目的 检测表皮生长因子样结构7基因（EGFL7）、微血管密度（MVD）、磷酸化局部黏着斑激酶（FAKpY397）在人脑胶质瘤中的表达，探讨胶质瘤 EGFL7 与 FAKpY397 及 MVD 的相互关系。方法 应用免疫组化法检测56例脑胶质瘤和8例脑外伤内减压脑组织中 EGFL7、FAKpY397的表达,用 CD34 标记计数MVD。结果 EGFL7在胶质瘤组织和正常脑组织中的阳性表达率差异有统计学意义 (P<0.01)；随着胶质瘤恶性程度的增加，EGFL7的表达也明显增强(P<0.01)。FAKpY397在胶质瘤组织和正常脑组织中的阳性表达率差异有统计学意义(P<0.05)；随着胶质瘤恶性程度的增加，FAKpY397的阳性率表达相应增高（P<0.01）。MVD计数在胶质瘤组织和正常脑组织中差异有统计学意义(P<0.01)，Ⅲ、Ⅳ级胶质瘤的MVD计数明显高于Ⅰ、Ⅱ级 (P<0.01)。EGFL7与FAKpY397在胶质瘤的表达呈正相关(P<0.01)；EGFL7阳性表达与阴性表达二组之间的MVD计数差异有统计学意义（P<0.01）。结论 EGFL7表达与胶质瘤的病理分级、MVD及FAKpY397均有良好的正相关性，提示EGFL7基因不仅在胶质瘤血管生成中起着重要的调控作用，而且可能直接参与了胶质瘤的发生、侵袭发展。

关键词:神经胶质瘤,表皮生长因子样结构7基因,微血管密度

Abstract:Objective To test the expression of epidermal growth factor-like domain 7 (EGFL7), microvessel density (MVD) and foeal adhesion kinase pY397 (FAKpY397) in human glioma tissues, and to evaluate their relationship. Methods The expression of EGFL7 and FAKpY397 in 56 cases of human glioma and 8 cases of normal brain tissues were detected by immunohistochemistry test, and MVD was detected by CD34 staining. Results There was a significant difference of the positive rates of EGFL7 between normal brain tissue and gliomas (P<0.01). With the increased pathological grade, the expression level of EGFL7 increased (P<0.01). There was a significant difference of the positive rates of FAKpY397 between normal brain tissue and gliomas (P<0.05). With the increased pathological grade, the expression level of FAKpY397 increased (P<0.01). There was a significant difference on MVD between normal braintissueand gliomas (P<0.01). Higher level of MVD was found in gliomas with higher grade (P<0.01). There was a positive correlation between EGFL7 and FAKpY397 expressions in gliomas (P<0.01). There was a significant difference on MVD between positive and negative expression of EGFL7 (P<0.01). Conclusion The expression of EGFL7 of human gliomas has a favorable positive correlation with the degree of malignancy, MVD and FAKpY397. It is indicated that EGFL7 not only palys an important regulative role in glioma neovascularization, also it may participate directly in glioma occurrence and invasion.