The chromatographic column was Thermo C₁₈（4.9 mm × 250 mm， 5 μm）， the flow phase was used with a methanol and 0.1% phosphoric acid， gradient elution and the flow rate was 1 mL/min， and the detection wavelength was set at 254 nm， and the column temperature was 30 ℃， and sample quantity was 10 μL.

Totally 16 chromatographic peaks were recognized as common peaks in the fingerprints through the analysis of similarity software. And the components of aloe emodin， rheinemodin， chrysophanol and physcion were identified by this method with similarities at 0.691 ~ 0.98. Similarity evaluation combined with SPSS cluster analysis（CA） and Principal Component Analysis （PCA） was used to evaluate the fingerprint of 10 batches of extractumrheiand five markers were quantified.

The established method for the quantitative determination of fingerprint of extractumrheiis accurate， simple and specific. At the same time， it can be effectively applied to the quality control of extractumrhei.