To optimize the prognosis assessment of breast cancer patients by developing proteomics signatures.

The protein expression profiles and clinical information of 879 breast cancer （BC） patient samples were downloaded from the TCPA and TCGA databases， respectively. We performed univariate Cox regression， lasso regression and multivariate Cox regression analysis to identify prognostic proteins and establish a prognostic model. Then， we further analyzed the differential expression and survival of the prognostic model protein， and analyzed the predictive performance of the model risk score. In addition， we analyzed all proteins related to prognostic model proteins.

Based on the 224 proteins in the TCPA database， we constructed a prognostic model containing 6 proteins （CASPASE7CLIVEDD198， NFKBP65_pS536， PCADHERIN， P27， X4EBP1_pT70， EIF4G）， and divided the patients into two groups according to the median of risk score. The overall survival rate was significantly different. Univariate and multivariate Cox regression analysis showed that the risk score was an independent prognostic factor for patients （P< 0.001）. In addition， the nomogram and ROC are used to demonstrate the predictive performance of the predictive model. The protein interaction relationship showed that the proteins CASPASE7CLEAVEDD198， PCADHERIN， X4EBP1_pT70 and EIF4G showed obvious correlation with other proteins （P< 0.001）.

Studies have shown that six key proteins may be considered as new targets for BC treatment， and the protein risk model can be used to predict the overall survival of BC patients.

To systematically evaluate the efficacy and safety of Wuling capsule combined with Deanxit in the treatment of post-stroke depression （PSD）， and to provide reference for the clinical application of drugs to prevent PSD.

Randomized controlled trial （RCTs） of Wuling caspule combined with Deanxit in the treatment of post-stroke depression were searched from CNKI，VIP，WanFang，PubMed and other datebases by electronic computer， and the deadline was September 2020.After the two researchers independently screened the literature， extracted the data and comprehensively evaluated the risk of bias included in this study， a Meta-analysis was performed on all the results using the Revnew 5.3 software.

A total of 23 RCTs were included， including 2059 patients （1033 patients in the experimental group and 1026 patients in the control group）.Meta-analysis results showed that the total effective rate of PSD patients in the experimental group was higher than that in the control group， with statistical significance （RR= 1.19，95%CI：1.14～1.24，P< 0.01）.The Hamilton depression scale （HAMD） score of patients with PSD reduction in the experimental group was significantly higher than that of the control group and was statistically significant （MD= －4.74，95%CI：－5.13～－3.75，P< 0.01）.The NIHSS score of patients with reduced PSD in the experimental group was higher than that in the control group and was statistically significant （MD= －2.33， 95%CI： －3.63～－1.02，P< 0.01）.The Barthel index （BI） score of patients with increased PSD in the experimental group was higher than that of the control group and was statistically significant （MD= 7.09， 95%CI： 4.78～9.41，P< 0.01）.The incidence of adverse reactions in the experimental group was significantly lower than that in the control group and was statistically significant （RR= 0.54，95%CI：0.41～0.70，P< 0.01）.

Compared with Deanxit， Wuling capsule combined with Deanxit can significantly improve the curative effect of PSD， improve the neurological function， daily life ability， and have fewer side effects.

Enterovirus 71 （EV71） is the main pathogen causing severe hand， foot and mouth disease （HFMD）， however， there is no effective antiviral drugs available. To screen mouse specific B cells expressing antibodies against EV71 by using EV71 VP1 protein as an antigen.

The codon-optimized EV71 VP1 gene was cloned into pET-21a （+） expression vector and transformed intoE. coli.BL21-DE3. By optimizing protein expression and renaturation conditions， the expressed protein was confirmed by SDS-PAGE and Western blot and its antigenicity was also tested. Using purified protein as antigen， B lymphocytes of mice immunized with inactivated， and EV71 viruses were sorted by flow cytometry for the preparation of monoclonal antibody.

The EV71 VP1 gene was stably expressed in the form of inclusion body in theE. coli. The purified protein showed good immunogenicity， and there was a significant difference between the B cells of immunized and unimmunized BALB/c mice when screened using the expressed protein.

The recombinant EV71 VP1 protein with good immunogenicity is successfully obtained and combined with flow cytometry， it can be used for detection and sorting of EV71 specific B cells.

To investigate the alleviation effects of drinking hydrogen-rich water on the oral mucosal ulcer induced by 5-fluorouracil （5-FU） and its possible biological mechanism.

Male SD rats were intraperitoneally injected with 5-FU （0.05 mg/g） for 2 consecutive days. On D3， rats were treated with NaOH particles or 20% acetic acid on buccal mucosa to establish oral ulcer model. From D5， the rats were given hydrogen-rich water， and the animals were sacrificed on the D10. The survival rates of rats in all experimental groups were counted， and the lesions of oral mucosa were measured by Image J software. The levels of TNF-α， IL-6 and IL-1β in serum were detected by ELISA， and the expressions of TNF-α， IL-6 and iNOS at buccal mucosa were detected by immunohistochemistry.

Drinking hydrogen-rich water improved the survival rate of rats in the experimental groups. The damage area of ulcers produced by NaOH particles were larger than that caused by injection with 20% acetic acid （P< 0.05）， but there was no significant difference in the damage area of ulcers with drinking hydrogen-rich water or not （P> 0.05）. The serum levels of TNF-α， IL-6 and IL-1β of rats in model groups were significantly higher than those in the control group， while levels of the hydrogen-rich water group showed a downward trend compared with the model group. The expression of TNF-α and IL-6 at buccal mucosa were low in the healthy control and high in the model groups， while the expression in hydrogen-rich water groups showed a downward trend compared with the model group. The expression of iNOS at buccal mucosa was low in all groups.

Molecular hydrogen alleviates oral mucosal ulcers induced by 5-FU in rats possibly through inhibiting oxidative stress-related inflammation.

To find out key functional genes and molecular mechanisms related to the differential seizure frequency of temporal lobe epilepsy.

Different seizure frequency epilepsy transcriptome sequencing data GSE127871 were downloaded from GEO database， R language DESeq2 package was used for differential gene expression analysis，clusterProfiler package for Gene Ontology and Kyoto Gene and Genome Encyclopedia pathway enrichment analysis； STRING database was used to analyze the protein interaction network of differential genes； Cytoscape was used to screen key genes from the protein level.

A total of 820 differentially expressed genes were analyzed through transcriptome data， including 332 up-regulated genes and 488 down-regulated genes. The biological processes of differentially expressed genes Gene Ontology were significantly enriched in membrane potential regulation and chemical and ion channel regulation of transmembrane transduction， neurotransmitter level regulation， etc； differential expressed gene Gene Ontology cell components were significantly enriched in synapses， neurosomal bodies， transmembrane transduction complexes， etc； Gene Ontology molecular functions were significantly enriched on positive activation of transmembrane transduction， activation of ion channels and gated channels， etc； KEGG pathway analysis was significantly enriched in neural activation ligand-receptor interaction， cholinergic synapses， and NF-kappaB signaling pathway etc. Protein interaction network analysis and hub gene screening showed that ADCY1， MCHR1， CXCL8， CXCL1， CXCL2 and CXCL3 genes were important hub genes；

The molecular mechanism of differentially expressed genes in temporal lobe epilepsy transcriptome with different seizure frequencies is mainly related to signal transduction of chemical neurotransmitters and ion channels between synapses . Studies have confirmed that ADCY1， MCHR1， CXCL8， CXCL1， CXCL2， CXCL3 and other genes are the key hub genes for temporal lobe epilepsy with different seizure frequencies.

To analyze their therapeutic effects ofVMRT and IMRT on patients with glioma.

Ninety-four patients with glioma treated in our hospital from February 2018 to April 2019 were selectedand divided them into two groups. There were 47 cases in the control group and 47 cases in the control group；the 47 cases in the observation group were treated with VMRT. The HI，CI，clinical efficacy and survival rate of the two groups of patients were compared.

The CI of the observation group was higher than that of the control group（P< 0.05），and there was no significant difference in HI between the two groups（P> 0.05）；the objective remission rate of the observation group was 80.85%，and the control group was 61.70%. The difference was significant （P< 0.05）；the half-year，one-year，and two-year survival rates of the observation group were 80.85%，72.34%，and 53.19%， respectively， and the control group were 59.57%，42.55%，and 29.79%，respectively，with significant differences （P< 0.05） .

The CI of VMRT is better than IMRT for patients with glioma. The objective response rate is higher and the survival rate within 2 years is higher， which is worthy of promotion.

To investigate the effects of CDDO-EA （CDDO-ethylamide）， a novel Nrf2 related factor 2 （Nrf2）?， on neurological deficit and microglia polarization after subarachnoid hemorrhage （SAH） in mice.

Thirty healthy C57BL/6 mice were randomly divided into sham group， SAH + PBS group and SAH + CDDO-EA （50 μg） group. The SAH model was made by middle cerebral artery puncture， and the mice were sacrificed 3 days after operation for neurological function score. Brain tissue was collected for frozen sections and the M2-type microglia cells were labeled with CD206/Iba1 by immunofluorescence double staining.

The cerebral cortex of each group was collected 48 h after operation to detect the expression of CDDO-EA by Western blot. Compared with PBS control group， the expression of Nrf2 in brain of 50 μg and 100 μg treatment groups was significantly increased （P< 0.05）.The mice received PBS or CDDO-EA（50 μg） after surgery by intraperitoneal injection. The neurological function score was evaluated 72 h after surgery. SAH mice had obvious neurological dysfunction， but the neurological function was improved after CDDO-EA treatment （P< 0.05）. In addition， the numberof M2 microglia in cerebral cortex and hippocampus after CDDO-EA treatment significantly increased than that in vehicle control group.

CDDO-EA treatment attenuates neurological deficits after SAH， which may be achieved by up-regulating the expression of Nrf2 and promoting microglia polarization toward M2 phenotype.

To observe the distribution of blood lipids during different gestation periods in Wujiang District of Suzhou City， to establish the reference range of this region， and to provide reference for clinicians to make decisions.

A total of 1388 pregnant women with single pregnancy who were registered in our hospital until their delivery to full term were selected as the research subjects. The blood triglyceride， total cholesterol， low density lipoprotein and high density lipoprotein of 11 to 13⁺⁶weeks of pregnancy， 28 to 31⁺⁶weeks of pregnancy， 35 to 36⁺⁶weeks of pregnancy and 38 to 41⁺⁶weeks of pregnancy were analyzed statistically to establish the reference range of blood lipids of pregnant women at various stages of pregnancy in the local area.

The level of blood lipid during pregnancy gradually increased with pregnancy and obviously exceeded the level of non-pregnancy after the middle and late pregnancy.

Pregnant women have physiological blood lipid elevation， and the reference range of normal values in this area is defined by percentile method， so as to provide clinicians with reasonable interpretation of blood lipid results at different stages of pregnancy.

To explore the best preparation process of the double flower health oral liquid and to evaluate the protective effect of the double flower health oral liquid on stress-induced liver injury.

Important active components were extracted from ethanol extract of honeysuckle and chrysanthemum， the best conditions for the preparation of oral liquid oral liquidand suitable accessories were explored by designing the orthogonal experiment， and whether the oral liquid met the quality standards was detected .The effect of honeysuckle and chrysanthemum double flower oral liquid on liver stress induced liver injury in mice was further explored to evaluate the protective effect of the double flower health oral liquid on the induced liver injury. Establishment of stress liver injury mouse model by restraint load method， the activities of ALT in serum and the content of SOD and MDA in liver were determined to evaluate the hepatoprotective effects.

Orthogonal test showed that the best preparation technology of the double flower oral liquid was： 8% the mixture of flower extract （honeysuckle∶chrysanthemum = 1∶1）， 4% sucrose and 0.10% citric acid. The oral liquid prepared by this process accords with the quality standard stipulated in the Pharmacopoeia. The experimental results showed that the double flower health oral liquid had certain protective effects on induced liver injury， reduced the level of ALT， increased the level of SOD and reduced the content of MDA.

Through orthogonal test， the best prescription condition of the double flower health oral liquid is optimized， which accords with the quality standard of Chinese Pharmacopoeia. At the same time， the double flower health oral liquid can protect and improve stress liver injury in mice.

To investigate the values of the differential diagnosis of peripheral blood neutrophil/lymphocyte ratio （NLR）， platelet/lymphocyte ratio （PLR） and systemic immune inflammation index （SII） in healthy people， benign and malignant thyroid nodules.

172 patients with benign thyroid nodules and 285 patients with malignant nodules diagnosed in the Second People's Hospital of Liaocheng from January 2017 to October 2019 were selected as the research objects， and 199 patients with health examinations in the hospital during the same period were selected as the control group， the preoperative peripheral blood neutrophil count， lymphocyte count and platelet count were recorded， and NLR， PLR and SII were calculated， and NLR， PLR and SII in healthy people， benign nodules， and malignant nodules were compared byttest for the difference between groups， the receiver operating characteristic curve （ROC curve） was used to determine the critical values of NLR， PLR， and SII in different groups.

Except that there was no statistically significant difference between NLR and SII in the comparison of benign and malignant nodules （group Avsgroup B）， the three indicators （NLR， PLR， SII） in the other three groups were compared with each other， and the differences were statistically significant （P < 0.05）. The area of AUC was between 0.72 and 0.8. The sensitivity of NLR， PLR， and SII in the identification of healthy people and benign thyroid nodules was 85.9%， 92.5%， and 81.9% respectively. In the detection of healthy people and malignant thyroid nodules， the sensitivity in the discrimination was 70.4%， 81.9%， and 74.1%， respectively. The discrimination specificities in the benign nodule group and the malignant nodule group were 85.6%， 69.5%， and 83.2%， respectively， and the significance was significant.

The detection of NLR， PLR， and SII has a certain value to distinguish between healthy people and benign nodules and healthy people and thyroid nodules， but it is not significant to distinguish between benign and malignant nodules.

To evaluate the efficacy of Gandou Decoction in the treatment of hepatolenticular degeneration through Meta analysis.

Theonlinesearch for Gandou Decoction for treatment of liver peas and randomized controlled trial study of nucleus degeneration was made in the Chinese Journal Full-text Database （CNKI）， VIP Chinese Science and Technology Journal Full-text Database （VIP）， Chinese Academic Journals Database （Wanfang），Chinese Biomedical Literature Database （CBM），Pubmed in combination with manual search. After selecting the literature according to the inclusion and exclusion criteria，data extraction and quality evaluation，the RevMan5.3 software provided by the Cochrane Collaboration was used for meta-analysis.

A total of 10 RCT studies were included，including 639 patients. The results of Meta analysis showed that Gandou Decoction was better than the western medicine group in terms of total effective rate，improvement of liver function，and 24 h urinary copper excretion in the treatment of hepatolenticular degeneration.

The treatment principle of hepatolenticular degeneration is to drive copper and protect the liver. According to the results of the study，Gandou Decoction has thedefinite effect of removing copper and protecting the liver. However，the current research evidence is still insufficient，and further studies with larger samples and higher quality are needed in the future in order to clarify the therapeutic effect of Gandou Decoction.

To understand the status of anxiety and blood pressure control behavior of patients with hypertension in parts of rural areas，andto analyze the relations among the anxiety，blood pressure control behavior and blood pressure.

277 patients with hypertension were selected from three administrative villages of Jieshou City in Anhui Province，self-made questionnaire and self-rating anxiety scale（SAS） were used to conduct face to face survey.

The mean of the total score of anxiety scale was （38.27 ± 11.059），the detection rate of anxiety disorders was 14.9%.Comprehensive classification of blood pressure，increase exercise， age group and BMI classification were positively correlated with the total anxiety scores or dimension scores（P< 0.05），but dietcontrol and presence of complications were negatively correlated with the total anxiety scores or dimension scores（P< 0.05）.Dysfunction of the body，dietcontrol，smokingcontrol， moodregulation，going out or not and age group of hypertension diagnosis were positively correlated with comprehensive classification of blood pressure or diastolic and systolic blood pressure levels（P< 0.05），but mood disorder，mixed cross symptom，drinking control and age group were negatively correlated withcomprehensive classification of blood pressure or diastolic and systolic blood pressure levels（P< 0.05）.Comprehensive classification of blood pressure was the hinder factor of control diet（OR= 1.537 or 1.543）.The total anxiety scores was the promotion factor of control diet〔OR（95%CI） = 0.959（0.919～1.000）〕，but was the hinder factor of increasing exercise〔OR（95%CI） = 1.032 （1.004～1.060）〕.Mixed cross symptom is the promotion factor of smokingcontrol〔OR（95%CI）= 0.805（0.683～0.949）〕and drinkingcontrol〔OR（95%CI） = 0.823（0.698～0.971）〕.

Anxiety affects blood pressure， and its behaviorcontrol and blood pressure also affect anxiety and behaviorcontrol.In order to control blood pressure better， we should relieve patients' bad mood and promote the implementation of blood pressure control behavior.

To investigate the expression and clinical significance of chemokine 8 （CXCL8） and forkhead box P3 （FOXP3） in the serum of AIDS patients.

Seventy-two AIDS patients who came to our hospital from May 2018 to May 2021 were selected as the AIDS group， and 60 healthy people who came to our hospital for physical examination during the same period and matched with the general information of the patients were selected as the control group. The general data were collected， and the age， gender， T lymphocyte subgroup CD4⁺， CD8⁺， CD4⁺/CD8⁺levels and serum immunoglobulin IgG， IgM， IgA levels of the two groups were compared. Real-time quantitative fluorescent PCR （qRT-PCR） method was used to detect the expression level of FOXP3 mRNA in serum， enzyme-linked immunosorbent （ELISA） method was used to detect the expression level of CXCL8 in serum， and Pearson method was used to analyze the correlation between CXCL8 and FOXP3 expression. Receiver operating characteristic curve （ROC） was used to evaluate the diagnostic value of serum CXCL8 and FOXP3 levels for AIDS.

Compared with the control group， the serum CXCL8 expression level in the AIDS group was significantly up-regulated， and the FOXP3 mRNA expression level was down-regulated， the difference was statistically significant （P< 0.05）， and the CXCL8 and FOXP3 mRNA expression levels were significantly negatively correlated （r= －0.350，P< 0.05）； the levels of CD4⁺， CD8⁺， CD4⁺/CD8⁺in serum of AIDS patients were significantly lower than those of healthy people， and the levels of IgG， IgM and IgA were significantly higher than those of healthy people， which showed statistical significance （P< 0.05）； CXCL8 expression was negatively correlated with CD4⁺， CD8⁺， CD4⁺/CD8⁺（P< 0.05）， positively correlated with IgG， IgM， and IgA （P< 0.05）， FOXP3 mRNA was positively correlated with CD4⁺， CD8⁺and CD4⁺/CD8⁺expressions （P< 0.05）， and negatively correlated with the expressions of IgG， IgM and IgA （P< 0.05）. The combined prediction of serum CXCL8 and FOXP3 levels in AIDS patients was 0.981 （95%CI： 0.940 ~ 0.997）， with sensitivity at 98.61% and specificity at 91.67%， which were significantly higher than CXCL8 and FOXP3 alone.

CXCL8 is highly expressed in the serum of AIDS patients， and FOXP3 is low. The two are obviously negatively correlated， and are closely related to the occurrence and development of AIDS， which are of great significance for the diagnosis of AIDS patients.

To evaluate the effects of diffuse excessive high signal intensity （DEHSI） and periventricular leukomalacia （PVL） on brain development maturity of premature infants by total maturational scores （TMS）.

Retrospective analysis was performed on the craniocerebral MR images of 68 neonates， which were divided into three groups： normal preterm infants group， DEHSI group and PVL group （adjusted gestational age： 37－44 weeks）. The craniocerebral MR images of each newborn were evaluated by the TMS. The TMS scores were compared between the groups.

There were statistically significant differences in myelination， cortical infolding and TMS scores between the normal preterm infants and the DEHSI preterm infants （P< 0.05）；In the scores of myelination， cortical infolding and TMS， the order from high to low was normal preterm infants group， PVL infants group， DEHSI preterm infants group；There were no statistically significant differences in germinal matrix and migrating glial cells scores between any two groups （P> 0.05）；

The presence of DEHSI and PVL in the white matter of preterm infants affects the developmental maturity of the brain， and DEHSI and PVL have different effects on the developmental maturity of the brain. DEHSI does delay the developmental maturity of the brain.

To investigat the effect of follistation（FST） expression on hypopharyngeal carcinoma（HPC） tissues and cell lines.

A total of 60 patients with HPC in the Yijishan Hospital of Wannan Medical College from August 2010 to December 2017 were recruited for this study. Levels of FST mRNA and protein were measured by quantitative polymerase chain reaction （PCR） and immunohistochemistry in HPC tissue samples and by qPCR in the HPC FaDu cells， as well as immortal nasopharyngeal epithelial cell line NP-69 cells. After silencing the FST expression in FaDu cells using lentivirus-mediated siRNA that was specific for FST mRNA， cell proliferation was determined by the Celigo assay. Tumor growth was monitored in nude mice and viability was determined by MTT assay. The ratio of cell cycle arrest and apoptosis was carried out by flow cytometry. The colony formation ability was performed using Giemsa staining. In addition， wound healing assay were performed for analysis of cell motility. Furthermore， the putative mechanism of the effect of FST knockdown was analyzed using PathScan stress and apoptosis signaling array kit， and qPCR.

FST expression was significantly higher in human HPC tissue and FaDu cells when compared with normal tissue and NP-69 cells. A higher expression of FST in HPC samples was positively correlated with advanced tumors. Moreover， FST knockdown by shRNA significantly decreased cell growth， colony formation， and migration. Furthermore， FST silencing increased the cell apoptosis percentage， and arrested cell cycle in the G0/G1 and S phases in FaDu cells. FST silencing also suppressed tumor growthin vivoand might involve upregulating ERK1/2 and caspase signals.

Our results indicate that the FST gene is associated with HPC progression， and may serve as a potential therapeutic target for the treatment of HPC.

To analyze the financing level and structure of the total health expenditure of 31 provincial administrative regions in Inland China from 2014 to 2018 from the perspective of time and space，and to discuss the regional differences.

The longitudinal changes of health financing level and structure in China were analyzed. Meanwhile， ArcGIS10.2 software was used to conduct spatial autocorrelation analysis on health financing structure data of 31 provinces.

From 2014 to 2018， China's health financing level was constantly improved， and the financing structure was optimized， but the regional differences between provinces were still obvious.

In the future， the focus of China's health work will still need to improve the level of health financing， optimize the financing structure， analyze specific roblems， pay attention to the regional differences of health financing structure， and solve the unfairness of health financing.

To explore the relationship between blood manganese concentration and the incidence of cardiovascular diseases （CVDs） as well as individual CVDs in adults.

We initially integrated the data in National Health and Nutrition Examination Survey （NHANES） from 2011 to 2018. 4 005 adults agd between 20 and 60 years old were recruited based on the inclusion and exclusion criteria. The relationship between manganese concentration and the prevalence of CVD was analyzed by constructing generalized linear models and restricted cubic spline curves.

Compared with the lowest quartile， the modified odds ratios （ORs） with 95% confidence intervals （CIs） for CVD prevalence across the manganese quartiles were 1.066 （0.699，1.699）， 1.105 （0.639，1.613）， and 0.591 （0.376，0.930） respectively. In the full adjusted model， the incidence of total CVD decreased initially and increased when manganese concentration was over 9.59 μg/L. A restricted cubic spline curve was used to show a nonlinear “U” shape relationship between manganese concentration and CVD.

Based on adults， blood manganese concentration was associated with CVD prevalence. Maintaining manganese concentration at 9.59 μg/L can be beneficial for the heart.

To find serum markers which are easy to elevate， less invasive and at low cost in order to identify HCC at an early stage and take active and effective treatment by establishing a diagnostic model with multivariate regression analysis which helps to improve the patient′s cure rate and survival rate.

In this retrospective study， 188 patients with chronic HBV infection who were treated in Jinan Central Hospital Affiliated to Shandong First Medical University between 2017 and 2020 were enrolled. There are 150 patients with HBV infection in non-liver cancer group； 38 patients were included in the HBV infection related liver cancer group. Statistical analysis was performed using SPSS 25.0 and Stata se 15.0 software to compare the general data， conventional， biochemical and virological parameters detected after admission. The best model was selected by plotting receiver operating characteristic curve （ROC） and comparing the area under the curve （AUC） to calculate the sensitivity and specificity through univariate screening and multi-factor logical regression analysis.

In this study， we developed a logistic regression model of the combined serological indicators： SA， AST， GGT， AFP and HBsAg. The AUC was the largest at 0.943， which was significantly higher than the AUC = 0.816 （P= 0.004） of the single item of AFP with no cross-over with other curves. Sensitivity was 83.9%， and specificity was 92%.

For patients with HBV infection， the regression model established by comprehensive serological parameters： SA， GGT， AST， HBsAg and AFP has a good diagnostic efficiency for diagnosing HCC.

To elucidate the main active components and targets ofLonicerae japonicaeflos in the treatment of diabetes by network pharmacology， and to reveal the potential mechanism.

According to TCMSP， the active components and corresponding targets ofLonicerae japonicaeflos were analysis under the conditions of pharmacokinetic parameters ［oral bioavailability （OB） ≥ 30%， drug-likeness （DL） ≥ 0.18］. The diabetic targets of active components ofLonicerae japonicaeflos were screened by Gene cards and OMIM database. The “component-target-disease” network was constructed by Cytoscape 3.8.2， and the protein-protein interaction （PPI） network was established by String database. Then， the potential diabetic targets ofLonicerae japonicaeflos analysis of gene ontology （GO） function and Kyoto Encyclopedia of Genes and Genomes （KEGG） pathways were analyzed by R 4.0.4 language and Bioconductor. RCSB PDB and TMSCP database， Pymol and AutoDockTools-1.5.6 software were used for molecular docking ofLonicerae japonicaeflos core compounds and targets.

A total of 16 main active components were screened fromLonicerae japonicaeflos， and 120 targets were predicted for the treatment of diabetes. Go function analysis and KEGG pathway enrichment analysis showed that the mechanism ofLonicerae japonicaeflos in the treatment of diabetes mainly involves the biological processes such as DNA binding transcription factor binding， RNA polymerase Ⅱ specific DNA binding transcription factor binding， and it plays a role through multiple pathways such as lipid and arteriosclerosis， PI3K-Akt and TNF signaling pathway signaling pathway. Molecular docking results showed that luteolin， quercetin and kaempferol in the core compounds ofLonicerae japonicaeflos had high affinity for AKT1.

The anti-diabetes mechanism byLonicerae japonicae flos is the result of multi-component， multi-target and multi-channel， which provides a theoretical basis for the further study ofLonicerae japonicaeflos in the treatment of diabetes.

To explore and analyze the clinical efficacy of metformin combined with diet control exercise therapy in the treatment of community diabetes with high BMI.

Selected 76 cases of community diabetes patients with high BMI from February 2018 to March 2019， the patients were randomly divided into control group （n= 38） and observation group （n= 38），control group simply used metformin treatment，observation group used metformin combined diet control exercise therapy treatment，comparing the treatment effect of two groups of patients.

The fasting blood glucose （FPG） level，2h postprandial blood glucose （2 hPG） level，glycosylated hemoglobin （HbAlc） level，and body mass index （BMI） levels of the observation group were lower than those of the control group （P< 0.05）； the observation group was The total effective rate of the treatment was significantly higher than that of the control group，and the incidence of adverse reactions was lower than that of the control group. The difference between the groups was significant and statistically significant （P< 0.05）.

In the community diabetic patients with high BMI，the use of metformin combined with diet control exercise therapy can effectively reduce the patient's blood glucose level and body mass index level，the incidence of adverse reactions in patients is low， and the clinical treatment effect is significant. High promotion value.

To evaluate the effect of predictive nursing on Alzheimer's disease patients.

Databases from CNKI， Wanfang， VIP， CBM， Pubmed， Cochrane Library， Web of Science and Embase were collected， the search time limit was June 2020 in database building， and randomized controlled trials were included. Selected studies data were performed with RevMan 5.3 software.

A total of 17 articles were included， with a total sample size of 1366 cases. Meta-analysis results showed that predictive care could improve the quality of life of patients with Alzheimer′s disease［MD= 5.90， 95%CI（3.94， 7.86），P< 0.000 01］， and increase the effective rate of treatment ［RR= 2.40， 95%CI（1.99， 2.89），P< 0.000 01］， improve patient satisfaction ［RR= 2.25， 95%CI（1.64， 3.09），P< 0.000 01］， reduce patient complication rate ［RR= 0.23， 95%CI（0.13， 1.40），P< 0.000 01］.

The current evidence preliminarily shows that predictive nursing intervention for Alzheimer's disease may improve the quality of life of patients， improve the effectiveness of nursing and reduce complications. However， in view of the low quality of the included articles， high-quality， multi-center randomized controlled trials are needed for further verification support in the future.

To study the effect of interfering uncoupling protein 2 expression on apoptosis and autophagy induced by hypoxia.

The expressions of UCP2 in H9C2 myocardial cells after hypoxia were detected by RT-q PCR. Reactive oxygen species （ROS） fluorescence assay was used to detect the effect of UCP2 interference on intracellular ROS level. The apoptotic rate was detected by flow cytometry. The effects of UCP2 interference on cleaved-caspase 3 were further detected by Western blotting. The cell damage related factors were detected by ELISA： lactate dehydrogenase （LDH）， creatine kinase （CK）， interleukin-6 （IL-6） and tumor necrosis factor-alpha （TNF-a）. The expression levels of membrane LC3/cytoplasmic LC3 （LC3-?Ⅱ/LC3-?Ⅰ）， autophagy gene Beclin-1 and p62 were detected.

The expression of UCP2 was significantly increased in H9C2 cells under hypoxia. Interference with UCP2 expression could reduce the increase of ROS level in H9C2 cells induced by hypoxia. Interference with UCP2 expression could reduce the apoptotic rate of H9C2 cells and the expression levels of LDH， CK， IL-6 and TNF-alpha in hypoxic environment. In Western blotting， interfering with UCP2 expression could decrease the expression of LC3-?Ⅱ/LC3-?Ⅰ and Beclin-1， and increase the expression of p62.

Interference with UCP2 can reduce ROS level in cells induced by hypoxia， and inhibit apoptosis and autophagy induced by hypoxia.

To study the application effect of modified transurethral plasma resection of prostate in the treatment of high-risk prostatic hyperplasia.

A total of 80 high-risk patients with BPH admitted to our hospital from June 2019 to July 2020 were randomly divided into control group and observation group with 40 patients in each group.Forty patients in the control group were treated with conventional transurethral prostatic plasma electrotomy， while 40 patients in the observation group were treated with modified transurethral prostatic plasma electrotomy.Then statistical analysis was made on surgical indicators，Q-MAX，IPSS score，PVR，QOL score， postoperative complications and satisfaction of patients in the two groups.

The operation time and hospitalization time of patients in the observation group were significantly shorter than those in the control group， and the differences between the groups were statistically significant （P <0.05）. The amount of intraoperative bleeding and blood transfusion in the observation group were significantly less than those in the control group， and the differences between the groups were statistically significant （P< 0.05）.The increase of Q-MAX，QOL score，IPSS score and PVR in the observation group were significantly higher than those in the control group （P< 0.05）.The incidence of postoperative complications was 2.50% （1/40） in the observation group and 22.50% （9/40） in the control group，with the former significantly lower than the latter （P< 0.05）.In terms of satisfaction，the observation group was 92.50% （37/40） and the control group was 75.00% （30/40），the former was significantly higher than the latter （P<0.05）.

The modified transurethral plasma resection of prostate is very effective in the treatment of high risk prostatic hyperplasia，so it is worth promoting in the treatment of high risk prostatic hyperplasia patients.