Fascin1与E-cadherin在胃癌中的表达与意义

betway必威登陆网址（betway.com ）学报››2021,Vol. 42››Issue (8): 568-572.DOI:10.3969/j.issn.2097-0005.2021.08.002

Fascin1与E-cadherin在胃癌中的表达与意义

赵文兴¹(), 王振¹, 李磊², 于国庆³, 郑臻³, 史志猛²()

^1.泰安市中心医院普外二科，山东泰安 271000
^2.泰安市中心医院消化内科，山东泰安 271000
^3.betway必威登陆网址（betway.com ）研究生处，山东泰安 271016

收稿日期:2021-01-20出版日期:2021-08-25发布日期:2021-09-28
通讯作者:史志猛
作者简介:赵文兴，住院医师，硕士，主要从事临床消化道肿瘤诊治工作，E-mail：sdta20180102@163.com。
基金资助:
山东省医药卫生科技发展计划(2018WS155);泰安市科技发展计划(2018NS0239)

Expression and significance of Fascin1 and E-cadherin in gastric cancer

Wenxing Zhao¹(), Zhen Wang¹, Lei Li², Guoqing Yu³, Zhen Zheng³, Zhimeng Shi²()

^1.Dept. of General Surgery，Taian Central Hospital，Taian 271000，China
^2.Dept. of Gastroenterology，Taian Central Hospital，Taian 271000，China
^3.Dept. of Graduate，Shandong First Medical University & Shandong Academy of Medical Sciences，Taian 271000，China

Received:2021-01-20Online:2021-08-25Published:2021-09-28
Contact:Zhimeng Shi

摘要/Abstract

摘要： 目的

探讨Fascin1与E-cadherin在胃癌中的表达情况及与临床病理资料的关系，并分析两者的调控关系及与胃癌细胞侵袭转移的关系。

方法

免疫组化方法检测胃癌及癌旁组织中Fascin1与E-cadherin的表达情况并分析两种蛋白在胃癌中的表达与临床病理参数的关系及二者表达的相关性，进一步检测人胃癌SGC-7901细胞中Fascin1与E-cadherin的调控作用；细胞实验研究Fascin1与胃癌细胞侵袭迁移的关系。

结果

Fascin1在胃癌组织中表达显著高于癌旁组织，E-cadherin在胃癌组织中表达显著低于癌旁组织，胃癌中Fascin1与E-cadherin表达呈显著负相关，Fascin1、E-cadherin在胃癌组织中的表达均与性别、年龄无关，均与TNM分期、淋巴结转移、浸润深度相关；细胞实验中Fascin1敲低后，E-cadherin mRNA与蛋白表达水平明显增加；SGC-7901细胞中，Fascin1干扰后，细胞的侵袭迁移能力明显降低。

结论

Fascin1在胃癌中高表达，E-cadherin在胃癌中低表达，两者均与胃癌转移有关，Fascin1对E-cadherin具有负向调控作用，Fascin1可能参与了胃癌细胞EMT过程进而调节胃癌的转移。

关键词:Fascin1,E-cadherin,胃癌,上皮-间质转化

Abstract: Objective

To investigate the expression of Fascin1 and E-cadherin in gastric cancer and the relationship with the clinicopathological data，and to analyze the regulation relationship between Fascin1 and E-cadherin and the relationshipwith the invasion and metastasis of gastric cancer cells.

Methods

The expression of Fascin1 and E-cadherin was detected in gastric cancer and adjacent tissues by immunohistochemical method，and the relationship between the expression of two proteins in gastric cancer and the clinicopathological parameters and the correlation between the two proteins expressionin gastric cancer were analyzed，and the regulatory role ofFascin1 and E-cadherin was further detected in human gastric cancer SGC-7901 cells； The relationship between Fascin1 and invasion and migration of gastric cancer cells was researched by cell experiments.

Results

Theexpression of Fascin1 in gastric cancer tissues was significantly higher than that of paracancerous tissues.The expression of E-cadherin in gastric carcinoma was significantly lower than that of paracancer tissues.The expression of Fascin1 and E-cadherin in gastric cancer was negatively correlated.The expression of Fascin1 and E-cadherin in gastric cancer tissues was not related to sex and age. All of them were associated with TNM stage，lymph node metastasis，and invasion depth. After Fascin1 interference by siRNA，the mRNA and protein expression level of E-cadherin significantly increased in cell experiments，and the invasion and migration ability of cells significantly decreased in SGC-7901 cells.

Conclusion

Fascin1 was highly expressed in gastric cancer，and the expression of E-cadherin is lower in gastric cancer.Both of them are related to metastasis of gastric cancer.Fascin1 has negative regulation on E-cadherin.Fascin1 may be involved in the EMT process of gastric cancer cells and then regulation of the metastasis of gastric cancer.

Key words:Fascin1,E-cadherin,gastric cancer,EMT

中图分类号:

R735.2

赵文兴, 王振, 李磊, 于国庆, 郑臻, 史志猛. Fascin1与E-cadherin在胃癌中的表达与意义[J]. betway必威登陆网址（betway.com ）学报, 2021, 42(8): 568-572.

Wenxing Zhao, Zhen Wang, Lei Li, Guoqing Yu, Zhen Zheng, Zhimeng Shi. Expression and significance of Fascin1 and E-cadherin in gastric cancer[J]. Journal of Shandong First Medical Unversity & Shandong Academy of Medical Sciences, 2021, 42(8): 568-572.

图/表4

图1Fascin1与E-cadherin在胃癌及癌旁组织中的表达情况SABC法：A、B. Fascin1在胃癌组织中阳性表达（× 400）且Fascin1在胃癌组织中的阳性表达率明显高于癌旁组织（P < 0.001）；C、D. E-cadherin在胃癌及癌旁组织中阳性表达（× 400），且E-cadherin在胃癌组织中的阳性表达率明显低于癌旁组织（P < 0.001）。

表1Fascin1、E-cadherin在胃癌组织中的表达及其与临床病理参数的关系

临床参数		例数	Fascin1		P	E-cadherin		P
临床参数		例数	阳性	阴性	P	阳性	阴性	P
性别	男	67	28	39	0.888	25	42	0.877
性别	女	23	10	13	0.888	9	14	0.877
年龄	≥ 60岁	40	17	23	0.962	18	22	0.206
年龄	< 60岁	50	21	29	0.962	16	34	0.206
TNM分期	Ⅰ、Ⅱ期	72	26	46	0.019	32	40	0.019
TNM分期	Ⅲ、Ⅳ期	18	12	6	0.019	2	16	0.019
淋巴结转移	是	20	13	7	0.019	3	17	0.034
淋巴结转移	否	70	25	45	0.019	31	39	0.034
浸润深度	浆膜	65	32	33	0.030	18	47	0.001
浸润深度	未达浆膜	25	6	19	0.030	16	9	0.001

表1Fascin1、E-cadherin在胃癌组织中的表达及其与临床病理参数的关系

临床参数		例数	Fascin1		P	E-cadherin		P
临床参数		例数	阳性	阴性	P	阳性	阴性	P
性别	男	67	28	39	0.888	25	42	0.877
性别	女	23	10	13	0.888	9	14	0.877
年龄	≥ 60岁	40	17	23	0.962	18	22	0.206
年龄	< 60岁	50	21	29	0.962	16	34	0.206
TNM分期	Ⅰ、Ⅱ期	72	26	46	0.019	32	40	0.019
TNM分期	Ⅲ、Ⅳ期	18	12	6	0.019	2	16	0.019
淋巴结转移	是	20	13	7	0.019	3	17	0.034
淋巴结转移	否	70	25	45	0.019	31	39	0.034
浸润深度	浆膜	65	32	33	0.030	18	47	0.001
浸润深度	未达浆膜	25	6	19	0.030	16	9	0.001

图2Fascin1对E-cadherin mRNA及蛋白表达水平的影响SGC-7901细胞中，Fascin1敲低后，可以明显提高E-cadherin的mRNA（A，B）与蛋白表达水平（C，D）（*vs Normal：P < 0.05，#vs Control-siRNA：P < 0.05）。

图3Fascin1对SGC-7901运动能力的影响SGC-7901细胞中，敲低Fascin1的表达后，细胞的迁移（A，B）与侵袭（C，D）能力明显降低（*：P < 0.05）。

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